Enhanced BMP-2/BMP-4 ratio in patients with peripheral spondyloarthritis and in cytokine- and stretch-stimulated mouse chondrocytes

Briolay, Anne; Jamal, Alaeddine El; Arnolfo, Paul; Goff, Benoît Le; Blanchard, Frédéric; Magne, David; Bougault, Carole

doi:10.1186/s13075-020-02330-9

Cited by 10 publications

(7 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, irisin was also able to blunt the catabolic effect of 10 ng/mL IL‐1β and TNF‐α on hTC metabolism and oxidative stress in vitro. In previous studies, such a concentration has proven effective in inducing a catabolic proinflammatory response in hTCs by both cytokines in vitro 23,24 . Indeed, both the addition of irisin to hTCs cultured in proinflammatory conditions and the later introduction of IL‐1β and TNF‐α in a hTC culture preconditioned with irisin resulted in an increase of mitochondrial activity and decrease of NO concentration, until reaching levels encountered with the standard medium alone.…”

Section: Discussionmentioning

confidence: 94%

Irisin inhibits tenocyte response to inflammation in vitro: New insights into tendon‐muscle cross‐talk

Giacomo

Vadalà

Ambrosio

et al. 2023

Journal Orthopaedic Research

View full text Add to dashboard Cite

Tendinopathy is one of the most common musculoskeletal disorders with significant repercussions on quality of life and sport activities. Physical exercise (PE) is considered the first‐line approach to treat tendinopathy due renowned mechanobiological effects on tenocytes. Irisin, a recently identified myokine released during PE, has been recognized for several beneficial effects towards muscle, cartilage, bone, and intervertebral disc tissues. The aim of this study was to evaluate the effects of irisin on human primary tenocytes (hTCs) in vitro. Human tendons were harvested from specimens of patients undergoing anterior cruciate ligament reconstruction (n = 4). After isolation and expansion, hTCs were treated with RPMI medium (negative control), interleukin (IL)−1β or tumor necrosis factor‐α (TNF‐α) (positive controls; 10 ng/mL), irisin (5, 10, 25 ng/mL), IL‐1β or TNF‐α pretreatment and subsequent co‐treatment with irisin, pretreatment with irisin and subsequent co‐treatment with IL‐1β or TNF‐α. hTC metabolic activity, proliferation, and nitrite production were evaluated. Detection of unphosphorylated and phosphorylated p38 and ERK was performed. Tissue samples were analyzed by histology and immunohistochemistry to evaluate irisin αVβ5 receptor expression. Irisin significantly increased hTC proliferation and metabolic activity, while reducing the production of nitrites both before and after the addition of IL‐1β and TNF‐α. Interestingly, irisin reduced p‐p38 and pERK levels in inflamed hTCs. The αVβ5 receptor was uniformly expressed on hTC plasma membranes, supporting the potential binding of irisin. This is the first study reporting the capacity of irisin to target hTCs and modulating their response to inflammatory stresses, possibly orchestrating a biological crosstalk between the muscle and tendon.

show abstract

Section: Discussionmentioning

confidence: 94%

Irisin inhibits tenocyte response to inflammation in vitro: New insights into tendon‐muscle cross‐talk

Giacomo

Vadalà

Ambrosio

et al. 2023

Journal Orthopaedic Research

View full text Add to dashboard Cite

show abstract

“…It is also interesting that Yoda1 did not induce BMP2 in pCH-OA cells to the same extent as in healthy cells, meaning that activation of Piezo1 in OA cells appears to result in lower production of BMP2. Notably, serum and synovial fluid BMP2 levels were shown to be associated with radiographic and symptomatic severity of knee OA, allowing BMP2 to serve as an alternative biochemical parameter for determining the disease severity of primary knee OA [32,33]. Because it was shown that serum BMP-2 levels were higher in patients with knee OA than in healthy controls, our observation of a decrease in BMP2 expression in pCH-OA cells under Yoda1 could be interpreted as a protective effect in OA cells.…”

Section: Discussionmentioning

confidence: 99%

Activation of the Mechanosensitive Ion Channels Piezo1 and TRPV4 in Primary Human Healthy and Osteoarthritic Chondrocytes Exhibits Ion Channel Crosstalk and Modulates Gene Expression

Steinecker-Frohnwieser

Lohberger

Toegel

et al. 2023

IJMS

View full text Add to dashboard Cite

Osteoarthritis (OA) is the most common degenerative joint disease causing pain and functional limitations. Physical activity as a clinically relevant, effective intervention alleviates pain and promotes joint function. In chondrocytes, perception and transmission of mechanical signals are controlled by mechanosensitive ion channels, whose dysfunction in OA chondrocytes is leading to disease progression. Signaling of mechanosensitive ion channels Piezo/TRPV4 was analyzed by Yoda1/GSK1016790A application and calcium-imaging of Fura-2-loaded chondrocytes. Expression analysis was determined by qPCR and immunofluorescence in healthy vs. OA chondrocytes. Chondrocytes were mechanically stimulated using the Flexcell™ technique. Yoda1 and GSK1016790A caused an increase in intracellular calcium [Ca2+]i for Yoda1, depending on extracellularly available Ca2+. When used concomitantly, the agonist applied first inhibited the effect of subsequent agonist application, indicating mutual interference between Piezo/TRPV4. Yoda1 increased the expression of metalloproteinases, bone-morphogenic protein, and interleukins in healthy and OA chondrocytes to a different extent. Flexcell™-induced changes in the expression of MMPs and ILs differed from changes induced by Yoda1. We conclude that Piezo1/TRPV4 communicate with each other, an interference that may be impaired in OA chondrocytes. It is important to consider that mechanical stimulation may have different effects on OA depending on its intensity.

show abstract

“…In a study of mouse model with early peripheral spondyloarthritis, it was shown that the BMP-2/BMP-4 ratio increased 6.5 times in the peripheral joints as a result of cytokine induction. 30 In our study, we divided the patients into 2 groups as active and inactive according to their BASDAI scores and examined the level of biomarkers. As a result, BMP-2 values were significantly higher in active disease, while BMP-4 values were significantly lower.…”

Section: Discussionmentioning

confidence: 99%

The Relationship of Serum Levels of Potential Biomarkers Affecting Bone Formation with Radiological Progression and Disease Activation in Patients with Ankylosing Spondylitis

ÖZDEMİREL¹,

Güven²,

DOĞANCI³

et al. 2022

J PMR Sci

View full text Add to dashboard Cite

To determine the levels of Dickkopf-1, sclerostin, bone morphogenetic protein (BMP) -2 and 4, interleukin (IL)-17 and 23, which might contribute to the radiographic progression and disease activity in ankylosing spondylitis (AS). Material and Methods: A cross-sectional study was carried out on 238 AS patients and age and sex-matched control group of 102 individuals. The disease activity was assessed through the Bath Ankylosing Spondylitis Disease Activity Index (BASDAI). In both groups Dickkopf-1, BMP-2 and 4, sclerostin, IL-17 and 23 levels were measured. Radiographic changes were calculated based on the modified Stokes Ankylosing Spondylitis Spinal Score (mSASSS). Results: Dickkopf-1, sclerostin, IL-17 and 23 levels were significantly higher in AS group compared to the controls. There was no difference regarding serum BMP-4 levels, whereas BMP-2 levels were significantly higher in the control group (p<0.001). Mean mSASSS was 4.4±6.2 and it was determined that biomarkers alone did not affect this score in the evaluation made by taking all factors under control by variance analysis. However, BMP-2 and 4 values together above the median values affected the mSASSS by 3.3% (p=0.017). In the correlation analysis, a weak negative significant correlation was found between BASDAI and BMP-4 (p<0.05). Conclusion: There are many inflammatory and non-inflammatory pathways that contribute to radiographic progression in ankylosing spondylitis. Both the data in the literature and the results of our study point to the importance of the local level and functionality of markers that may contribute to progression rather than serum levels.

show abstract

Enhanced BMP-2/BMP-4 ratio in patients with peripheral spondyloarthritis and in cytokine- and stretch-stimulated mouse chondrocytes

Cited by 10 publications

References 65 publications

Irisin inhibits tenocyte response to inflammation in vitro: New insights into tendon‐muscle cross‐talk

Irisin inhibits tenocyte response to inflammation in vitro: New insights into tendon‐muscle cross‐talk

Activation of the Mechanosensitive Ion Channels Piezo1 and TRPV4 in Primary Human Healthy and Osteoarthritic Chondrocytes Exhibits Ion Channel Crosstalk and Modulates Gene Expression

The Relationship of Serum Levels of Potential Biomarkers Affecting Bone Formation with Radiological Progression and Disease Activation in Patients with Ankylosing Spondylitis

Contact Info

Product

Resources

About