Enhanced biosynthesis of dextransucrase: A multivariate approach to produce a glucosyltransferase for biocatalysis of sucrose into dextran

Optimization of Process Parameters for Maximum Production and Characterization of Dextransucrase From Newly Isolated Acetobacter Tropicalis

“…This value was far higher than those reported previously by different bacterial strains (Paul et al, 1984;Monchois et al, 1997;Funane et al, 2000;Fabre et al, 2005;Moulis et al, 2006;Joucla et al, 2006;Iliev and Vasileva, 2012;Rania et al, 2013;Miljkovic et al, 2017, Zafar et al, 2018. On the other hand, no contamination of extracellular levansucrase was observed during purification process.…”

“…The optimum fermentation time for L. mesenteroides FT 045, L. mesenteroides CMG713 and L. mesenteroides NRRL B-512 (F) were reported to be 8 h, 20 h and 6 h respectively (Cortezi et al, 2005;Sarwat et al, 2008;Honorato and Rodrigues, 2010). Whereas, 48 h and 8 h of incubation periods have been reported for maximum dextransucrase production by Lactobacillus acidophilus ST76480.01 and W. confusa (Rania et al, 2013;Zafar et al, 2018) Incubation time (h) …”

“…Sawale and coworkers applied response surface methodology (RSM) for enhanced dextransucrase production from strain L. mesenteroides UICT/LI18 which showed 5.42 folds increase in dextransucrase activity (8.15 U/ml) over the basal medium (Sawale et al, 2010b). Recently, it has been reported that dextransucrase production from W. confusa enhanced the enzyme yield from 0.54 U/mL to 2.51 U/mL with 4.6-fold upsurge after the implementation of the Plackett-Burman design (Zafar et al, 2018).…”