Enhanced Biosynthesis of D-Arabitol by Metschnikowia reukaufii Through Optimizing Medium Composition and Fermentation Conditions

Abstract

D -arabitol is an important functional sugar alcohol, which can be used in the preparation of foods, chemicals, and medicines. Despite biological production of D -arabitol from low-cost substrates has recently been the focus of research, low yield of this technology has limited its large-scale exploitation.Optimization of this bioprocess could be a promising option to improve the yield of D -arabitol. In this study, one-factor-at-a-time (OFAT) strategy and Box-Behnken design (BBD) were used to increase Darabitol production by Metschnikowia reukau i CICC 31858 through optimizing the fermentation conditions and medium composition. The OFAT optimization provided the optimal conditions for temperature, agitation speed, and fermentation time of 30℃, 220 rpm, and 144 h, respectively. Likewise, the optimum concentrations of peptone, ammonium sulfate, KH 2 PO 4 , MgSO 4 •7H 2 O, and fumaric acid in the fermentation medium were (g/L) 7.5, 1, 2 , 0.5 and 7.5, respectively. Under these optimum conditions, 80.43 g/L of D -arabitol was produced from 200 g/L of glucose, with a productivity of 0.56 g/L/h. The BBD optimization with three important components of fermentation medium (KH 2 PO 4 , MgSO 4 •7H 2 O and fumaric acid) showed that the predicted titer of D -arabitol varied from 47.21 g/L to 89.27 g/L, and the actual titer of D -arabitol ranged from 47.36 to 89.83 g/L. The optimum concentrations (g/L) of KH 2 PO 4 , MgSO 4 •7H 2 O, and fumaric acid in the fermentation medium were found to be 1.0, 0.5, and 4.7g/L, respectively. Under the optimum conditions, 92.45 g/L of D -arabitol was nally produced with the yield and productivity of 0.46 g/g and 0.64 g/L/h, respectively.

RETRACTED ARTICLE: Mutagenesis combined with fermentation optimization to enhance gibberellic acid GA3 yield in Fusarium fujikuroi

“…In addition, the medium composition and culture conditions have a great impact on the overall metabolic network of the strain, thus affecting the quantity and quality of secondary metabolites (Huang et al 2022 ). For example, it was found that low concentrations of nitrogen sources (50 kg N.ha( − 1 ) nitrate) would improve the cell growth of F. fujikuroi , thus improving productive characteristic of GA3 (Moszczynska et al 2011 ).…”

Mutagenesis combined with fermentation optimization to enhance gibberellic acid GA3 yield in Fusarium fujikuroi

“…Similarly combined mutagenesis using both nitrosoguanidine and 60 Co gamma rays, along with screening for resistance against the fungicide terbina ne resulted in a 11.87% increase of the GA3 titer in the best strain (Wang et al 2014). In addition, the medium composition and culture conditions have a great impact on the overall metabolic network of the strain, thus affecting the quantity and quality of secondary metabolites (Huang et al 2022). For example, it was found that high concentrations of nitrogen sources (nitrate, ammonium) and glucose would reduce the titer of GA3 (Moszczynska et al 2011).…”