Enhanced biosynthesis of CdS nanoparticles through Arabidopsis thaliana phytochelatin synthase-modified Escherichia coli with fluorescence effect in detection of pyrogallol and gallic acid

Zhang, Dingkun; Yamamoto, Toshiyoshi; Tang, Dewei; Kato, Yuriko; Horiuchi, Shiho; Ogawa, Shinya; Yoshimura, Esturo; Suzuki, Michio

doi:10.1016/j.talanta.2018.11.092

Cited by 20 publications

(11 citation statements)

References 56 publications

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“…Phytochelatins, the enzymatic products of phytochelatin synthase, are small soluble peptides that play an essential role in resisting heavy metal toxicity in plants [8][9][10][11]. Their potential role in nanoparticle synthesis has been reported [13][14][15][16]. In the present study, we proposed that the overexpression of the enzyme promoted the synthesis of phytochelatins in the host E. coli cells based on literature review and our own observations, although we didn't measure the phytochelatin synthase activity or the level of phytochelatins in the cells.…”

Section: Discussionmentioning

confidence: 78%

“…The thiols of cysteine residues in phytochelatins interact with metals with high affinity and form the phytochelatin-metal complex during detoxification of metals and metalloids in plants [4,8,11]. The contribution of phytochelatins to nanoparticle synthesis has been investigated [13][14][15]. We also developed a recombinant E. coli bacterium that expresses R. tropici phytochelatin synthase, and discovered that overexpression of R. tropici phytochelatin synthase in E. coli cells improved the bacterial heavy metal resistance, and increased yield of selenium nanoparticles when whole cells were used to synthesize nanoparticles [16].…”

Section: Introductionmentioning

confidence: 99%

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Enhanced Extracellular Synthesis of Gold Nanoparticles by Soluble Extracts from Escherichia coli Transformed with Rhizobium tropici Phytochelatin Synthase Gene

Yuan

Bomma

2021

Metals

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Phytochelatins, the enzymatic products of phytochelatin synthase, play a principal role in protecting the plants from heavy metal and metalloid toxicity due to their ability to scavenge metal ions. In the present study, we investigated the capacity of soluble intracellular extracts from E. coli cells expressing R. tropici phytochelatin synthase to synthesize gold nanoparticle. We discovered that the reaction mediated by soluble extracts from the recombinant E. coli cells had a higher yield of gold nanoparticles, compared to that from the control cells. The compositional and morphological properties of the gold nanoparticles synthesized by the intracellular extracts from recombinant cells and control cells were similar. In addition, this extracellular nanoparticle synthesis method produced purer gold nanoparticles, avoiding the isolation of nanoparticles from cellular debris when whole cells are used to synthesize nanoparticles. Our results suggested that phytochelatins can improve the efficiency of gold nanoparticle synthesis mediated by bacterial soluble intracellular extracts, and the potential of extracellular nanoparticle synthesis platform for the production of nanoparticles in large quantity and pure form is worth further investigation.

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Section: Discussionmentioning

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Enhanced Extracellular Synthesis of Gold Nanoparticles by Soluble Extracts from Escherichia coli Transformed with Rhizobium tropici Phytochelatin Synthase Gene

Yuan

Bomma

2021

Metals

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“…Additionally, CdS NPs were biosynthesized via Arabidopsis thaliana phytochelatin synthase-modified E. coli (CdS/AtPCS1-E. coli). Biosynthesis of CdS NPs was greatly enhanced through expression of the AtPCS1 gene inside the E. coli cells by the pET28b vector due to the generation of phytochelatins (PCs; (γ-Glu-Cys)n-Gly, n ≥ 2), which efficiently captured Cd 2+ ions [82].…”

Section: Purification Of Nps Synthesized By Microorganismsmentioning

confidence: 99%

“…Incubation of the cells in a culture medium supplemented with cadmium ions and cysteine resulted in CdS NPs forming on the cell wall[81].Additionally, CdS NPs were biosynthesized via Arabidopsis thaliana phytochelatin synthase-modified E. coli (CdS/AtPCS1-E. coli). Biosynthesis of CdS NPs was greatly enhanced through expression of the AtPCS1 gene inside the E. coli cells by the pET28b vector due to the generation of phytochelatins (PCs; (γ-Glu-Cys)n-Gly, n ≥ 2), which efficiently captured Cd 2+ ions[82].Tables 1-6 list the active molecules used for the synthesis of metal NPs in microorganisms.…”

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confidence: 99%

Synthesis of Metal Nanoparticles by Microorganisms

Kato

Suzuki

2020

SSRN Journal

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Metal nanoparticles (NPs), with sizes ranging from 1-100 nm, are of great scientific interest because their functions and features differ greatly from those of bulk metal. Chemical or physical methods are used to synthesize commercial quantities of NPs, and green, energy-efficient approaches generating byproducts of low toxicity are desirable to minimize the environmental impact of the industrial methods. Some microorganisms synthesize metal NPs for detoxification and metabolic reasons at room temperature and pressure in aqueous solution. Metal NPs have been prepared via green methods by incubating microorganisms or cell-free extracts of microorganisms with dissolved metal ions for hours or days. Metal NPs are analyzed using various techniques, such as ultraviolet-visible spectroscopy, electron microscopy, X-ray diffraction, electron diffraction, energy-dispersive X-ray spectroscopy, and X-ray photoelectron spectroscopy. Numerous publications have focused on microorganisms that synthesize various metal NPs. For example

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“…Metal nanoparticles have unique physical properties characterized by the quantum size effect. They are used in medical and engineering fields as drug delivery agents, catalysts for chemical reactions, environmental cleanup agents, and antibacterial agents [ 16 , 17 , 18 , 19 , 20 , 21 ]. Research has also considered the value of microorganisms in synthesizing economically important metals as a novel method that requires comparatively little energy and can be incorporated in bioremediation strategies.…”

Section: Introductionmentioning

confidence: 99%

Deposition of Lead Phosphate by Lead-Tolerant Bacteria Isolated from Fresh Water near an Abandoned Mine

Kato

Kimura

Kogure

et al. 2022

IJMS

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Specialist bacteria can synthesize nanoparticles from various metal ions in solution. Metal recovery with high efficiency can be achieved by metal-tolerant microorganisms that proliferate in a concentrated metal solution. In this study, we isolated bacteria (Pseudomonas sp. strain KKY-29) from a bacterial library collected from water near an abandoned mine in Komatsu City, Ishikawa Prefecture, Japan. KKY-29 was maintained in nutrient medium with lead acetate and synthesized hydrocerussite and pyromorphite nanoparticles inside the cell; KKY-29 also survived nanoparticle synthesis. Quantitative PCR analysis of genes related to phosphate metabolism showed that KKY-29 decomposed organic phosphorus to synthesize lead phosphate. KKY-29 also deposited various metal ions and synthesized metal nanoparticles when incubated in various metal salt solutions other than lead. The present study considers the development of biotechnology to recover lead as an economically valuable material.

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Enhanced biosynthesis of CdS nanoparticles through Arabidopsis thaliana phytochelatin synthase-modified Escherichia coli with fluorescence effect in detection of pyrogallol and gallic acid

Cited by 20 publications

References 56 publications

Enhanced Extracellular Synthesis of Gold Nanoparticles by Soluble Extracts from Escherichia coli Transformed with Rhizobium tropici Phytochelatin Synthase Gene

Enhanced Extracellular Synthesis of Gold Nanoparticles by Soluble Extracts from Escherichia coli Transformed with Rhizobium tropici Phytochelatin Synthase Gene

Synthesis of Metal Nanoparticles by Microorganisms

Deposition of Lead Phosphate by Lead-Tolerant Bacteria Isolated from Fresh Water near an Abandoned Mine

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