Enhanced bioremediation of n-alkane in petroleum sludge using bacterial consortium amended with rhamnolipid and micronutrients

Rahman, K. S. M.; Rahman, Thahira; Kourkoutas, Yiannis; Petsas, I.; Marchant, Roger; Banat, Ibrahim M.

doi:10.1016/s0960-8524(03)00114-7

Cited by 407 publications

(214 citation statements)

References 17 publications

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“…Although no direct evidence has been provided, it is likely that indigenous soil bacteria work cooperatively with WatG to degrade diesel oil in soils. This cooperation may be mediated by rhamnolipids produced by WatG, because it is known that phenanthrene is degraded in soils co-inoculated with phenanthrene-degrading and biosurfactant-producing bacteria [4] and that the amendment of rhamnolipids to non-sterile soil enhances n-alkane degradation [9].…”

Section: Discussionmentioning

confidence: 99%

Verification of Degradation of n-Alkanes in Diesel Oil by Pseudomonas aeruginosa Strain WatG in Soil Microcosms

Ueno

Hasanuzzaman²,

Yumoto

et al. 2006

Curr Microbiol

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Degradation of n-alkanes in diesel oil by Pseudomonas aeruginosa strain WatG (WatG) was verified in soil microcosms. The total petroleum hydrocarbon (TPH) degradation level in two bioaugmentation samples was 51% and 46% for 1 week in unsterilized and sterilized soil microcosms, respectively. The TPH degradation in the biostimulation was of control level (15%). The TPH degradation in aeration-limited samples was clearly reduced when compared with that in aeration-unlimited ones under both sterilized and unsterilized conditions. Addition of WatG into soil microcosms was accompanied by dirhamnolipid production only in the presence of diesel oil. These findings suggest that degradation of n-alkanes in diesel oil in soil microcosms would be facilitated by bioaugmentation of WatG, with production of dirhamnolipid, and also by participation of biostimulated indigenous soil bacteria

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Section: Discussionmentioning

confidence: 99%

Verification of Degradation of n-Alkanes in Diesel Oil by Pseudomonas aeruginosa Strain WatG in Soil Microcosms

Ueno

Hasanuzzaman²,

Yumoto

et al. 2006

Curr Microbiol

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“…After bacterial cells were removed by centrifugation at 3,700 r/min for 30 min at 4°C, the supernatant was acidified to pH 2.0 using 1 mol/L HCl, and the acidified supernatant was left overnight at 4 °C for complete precipitation of the biosurfactant. For purification of surface-active component(s), the supernatant was extracted with an equal volume of solvent made up of chloroform: methanol (2:1, V/V) [26]. The organic solvent was transferred to a round-bottom flask connected to a rotary evaporator to remove the solvent, and the residual material was considered to be crude biosurfactant.…”

Section: Production and Purification Of Biosurfactantmentioning

confidence: 99%

Biodegradation of n-hexadecane by bacterial strains B1 and B2 isolated from petroleum-contaminated soil

et al. 2012

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Two hydrocarbon-biodegrading bacterial strains, B1 and B2, were isolated from petroleum-contaminated soil collected from Tianjin, China. The strains were identified as Pseudomonas aeruginosa (B1) and Acinetobacter junii (B2). The degradation rate of n-hexadecane by B1 and B2 reached 96% and 78% respectively after 7 days, though the strains employed different mechanisms of degradation. The results showed that B2 was not able to use glucose as carbon source. B1 could produce glycolipid surfactants using glucose as the carbon source, according to the results of blue agar plate analysis and thin layer chromatography (TLC), and the bacterial culture of B1 had a high oil discharge and emulsification activity. Both B1 and B2 could produce biosurfactants with hexadecane as the sole carbon source, but their modes of action were different. The carbon source was found to affect the cell surface hydrophobicity. Cell surface hydrophobicity was poor with glucose as the carbon source, but enhanced when hexadecane was used as the carbon source.

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“…This technique seems to be effective because the indigenous bacteria are likely to be better adapted to the soil environment requiring treatment (Rahman et al 2003). However, biostimulation sometimes does not work well and may take longer because bacteria with the ability to degrade xenobiotics may be scarce at contaminated sites or because the high-concentration of xenobiotics reduces the activity of degrading microorganisms.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of bacteria from soil contaminated with diesel oil and the possible use of these in autochthonous bioaugmentation

Ueno

Ito²,

Yumoto

et al. 2007

World J Microbiol Biotechnol

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SummaryTwo bacterial species (isolates N and O) were isolated from a paddy soil microcosm that had been artificially contaminated with diesel oil to which extrinsic Pseudomonas aeruginosa, strain WatG, had been added exogenously. One bacterial species (isolate J) was isolated from a similar soil microcosm that had been biostimulated with Luria-Bertani (LB) medium. Isolates N and O, which were tentatively identified as Stenotrophomonas sp. and Ochromonas sp., respectively, by sequencing of their 16 S rRNA genes had no ability to degrade diesel oil on their own in any liquid medium. When each strain was cocultivated with P. aeruginosa strain WatG in liquid mineral salts medium containing 1% diesel oil, isolate N enhanced the degradation of diesel oil by P. aeruginosa strain WatG, but isolate O inhibited it. In contrast, isolate J, which was tentatively identified as a Rhodococcus sp., degraded diesel oil contained not only in liquid LB and mineral salts media, but also in paddy soil microcosms supplemented with LB medium. The bioaugmentation capacity of isolate J in soil microcosms contaminated with diesel oil was much higher than that of P. aeruginosa strain WatG. The possibility of using isolate J for autochthonous bioaugmentation is discussed.

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Enhanced bioremediation of n-alkane in petroleum sludge using bacterial consortium amended with rhamnolipid and micronutrients

Cited by 407 publications

References 17 publications

Verification of Degradation of n-Alkanes in Diesel Oil by Pseudomonas aeruginosa Strain WatG in Soil Microcosms

Verification of Degradation of n-Alkanes in Diesel Oil by Pseudomonas aeruginosa Strain WatG in Soil Microcosms

Biodegradation of n-hexadecane by bacterial strains B1 and B2 isolated from petroleum-contaminated soil

Isolation and characterization of bacteria from soil contaminated with diesel oil and the possible use of these in autochthonous bioaugmentation

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