Enhanced biomass production and lipid accumulation of Picochlorum atomus using light-emitting diodes (LEDs)

Hun, Chae; Kang, Chang-Han; Jung, Jang-Hyun; Jeong, Gwi‐Taek; Kim, Sung-Koo

doi:10.1016/j.biortech.2016.07.078

Cited by 31 publications

(12 citation statements)

References 19 publications

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“…Kang et al determined the effect of using wastewater and wavelength filters on microalgal productivity and lipid accumulation with open raceway ponds, and found that the lipid productivity was highest under blue wavelength, at least 46.8% higher than that under white wavelength [45].This may be because blue light can promote the synthesis of lipids [46,47] and has low energy consumption. lipid accumulation of P. atomus was 329% higher under blue light than that under green light [44]. was highest under blue wavelength, at least 46.8% higher than that under white wavelength [45].This 201 may be because blue light can promote the synthesis of lipids [46,47] and has low energy Furthermore, cellular compositions of microalgae are generally critical evaluation parameters in various applications.…”

Section: The Led Power Conversion Capability Of Microalgae Biofilmmentioning

confidence: 99%

“…Similar results have been reported for the suspended culture system. Ra et al evaluated the effects of LED wavelength on the lipid production of Picochlorum atomus with two-phase suspended cultivation, and found that the lipid accumulation of P. atomus was 329% higher under blue light than that under green light [44]. Kang et al determined the effect of using wastewater and wavelength filters on microalgal productivity and lipid accumulation with open raceway ponds, and found that the lipid productivity was highest under blue wavelength, at least 46.8% higher than that under white wavelength [45].This may be because blue light can promote the synthesis of lipids [46,47] and has low energy consumption.…”

Section: The Led Power Conversion Capability Of Microalgae Biofilmmentioning

confidence: 99%

“…Similar results have 195 been reported for the suspended culture system. Ra et al evaluated the effects of LED wavelength on 196the lipid production of Picochlorum atomus with two-phase suspended cultivation, and found that the 197 lipid accumulation of P. atomus was 329% higher under blue light than that under green light[44].…”

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confidence: 99%

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Light-Emitting Diode Power Conversion Capability and CO2 Fixation Rate of Microalgae Biofilm Cultured Under Different Light Spectra

Yuan

Wang

et al. 2020

Energies

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Microalgae biofilm-based culture has attracted much interest due to its high harvest efficiency and low energy requirements. Using light-emitting diodes (LEDs) as light source for microalgae culture has been considered as a promising choice to enhance the economic feasibility of microalgae-based commodities. In this work, the LED power conversion capability and CO2 fixation rate of microalgae biofilms (Chlorella ellipsoidea and Chlorella pyrenoidosa) cultured under different light spectra (white, blue, green and red) were studied. The results indicated that the power-to-biomass conversion capabilities of these two microalgae biofilms cultured under blue and white LEDs were much higher than those under green and red LEDs (C. ellipsoidea: 32%–33% higher, C. pyrenoidosa: 34%–46% higher), and their power-to-lipid conversion capabilities cultured under blue LEDs were 61%–66% higher than those under green LEDs. The CO2 fixation rates of these two biofilms cultured under blue LEDs were 13% and 31% higher, respectively, than those under green LEDs. The results of this study have important implications for selecting the optimal energy-efficient LEDs using in microalgae biofilm-based culture systems.

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Section: The Led Power Conversion Capability Of Microalgae Biofilmmentioning

confidence: 99%

Section: The Led Power Conversion Capability Of Microalgae Biofilmmentioning

confidence: 99%

See 1 more Smart Citation

Light-Emitting Diode Power Conversion Capability and CO2 Fixation Rate of Microalgae Biofilm Cultured Under Different Light Spectra

Yuan

Wang

et al. 2020

Energies

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“…Light spectral quality also plays a key role in facilitating photosynthesis. Absorption of irradiation corresponding to the absorption band of the algal chlorophyll can lead to enhanced photosynthesis [ 58 ]. In a recent study, batch process was attempted in an open thin-layer cascade photobioreactor for high-cell density cultivation of a saline microalga ( N. salina) , wherein maximal cell density of 50 g/L was obtained within 25 days [ 59 ].…”

Section: Bioprocesses For Algal Cultivationmentioning

confidence: 99%

Recent developments in synthetic biology and metabolic engineering in microalgae towards biofuel production

Jagadevan

Banerjee

et al. 2018

Biotechnol Biofuels

201

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In the wake of the uprising global energy crisis, microalgae have emerged as an alternate feedstock for biofuel production. In addition, microalgae bear immense potential as bio-cell factories in terms of producing key chemicals, recombinant proteins, enzymes, lipid, hydrogen and alcohol. Abstraction of such high-value products (algal biorefinery approach) facilitates to make microalgae-based renewable energy an economically viable option. Synthetic biology is an emerging field that harmoniously blends science and engineering to help design and construct novel biological systems, with an aim to achieve rationally formulated objectives. However, resources and tools used for such nuclear manipulation, construction of synthetic gene network and genome-scale reconstruction of microalgae are limited. Herein, we present recent developments in the upcoming field of microalgae employed as a model system for synthetic biology applications and highlight the importance of genome-scale reconstruction models and kinetic models, to maximize the metabolic output by understanding the intricacies of algal growth. This review also examines the role played by microalgae as biorefineries, microalgal culture conditions and various operating parameters that need to be optimized to yield biofuel that can be economically competitive with fossil fuels.

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“…Chlorella vulgaris , with a long history of use as a superfood supplement, accumulates lipids up to 53% of its dry weight under nutrient-depleted conditions [ 4 ]. Parachlorella kessleri, formerly called C. kessleri , and a cosmopolitan marine microalga Picochlorum atomus also accumulate lipids at > 50% of its dry weight [ 5 , 6 ]. In particular, Choricystis minor cultivated under nitrogen and phosphorus starvation accumulates lipids at a level of almost 70% of its dry weight in 10 days [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Cas9-guide RNA ribonucleoprotein-induced genome editing in the industrial green alga Coccomyxa sp. strain KJ

Yoshimitsu

Abe

Harayama

2018

Biotechnol Biofuels

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BackgroundOxygen-evolving photosynthetic microorganisms, collectively termed as microalgae, are gaining attention as alternative fuel sources. The unicellular alga Coccomyxa sp. strain KJ that belongs to the class Trebouxiophyceae can grow rapidly in minimal mineral media and accumulate triacylglycerols at levels > 60% (w/w) of its dry weight under nitrogen depletion conditions. Thus, the strain can be a good candidate for biofuel production. Still, substantial improvements in lipid productivity and other traits of this strain are needed to meet commercial production requirements. Consequently, the development of new genetic tools including genome editing that are applicable to this strain is highly desired.ResultsIn this paper, we report successful genome editing of strain KJ by intracellular delivery of a ribonucleoprotein complex comprising recombinant Cas9 protein and guide RNA. For introduction of Cas9-guide RNA ribonucleoprotein into strain KJ cells, we used an electroporator with a short (2.5 ms) electric pulse at a high field strength (7500 V cm−1) followed by multiple 50-ms electric pulses at low field strength (250 V cm−1). Under these conditions, we successfully isolated several knockout lines of the FTSY gene of strain KJ, encoding a signal recognition particle-docking protein at a frequency of 0.01%.ConclusionsOur study shows applicability of DNA-free genome editing in Coccomyxa, which may be applicable in other Trebouxiophyceae species.Electronic supplementary materialThe online version of this article (10.1186/s13068-018-1327-1) contains supplementary material, which is available to authorized users.

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Enhanced biomass production and lipid accumulation of Picochlorum atomus using light-emitting diodes (LEDs)

Cited by 31 publications

References 19 publications

Light-Emitting Diode Power Conversion Capability and CO2 Fixation Rate of Microalgae Biofilm Cultured Under Different Light Spectra

Light-Emitting Diode Power Conversion Capability and CO2 Fixation Rate of Microalgae Biofilm Cultured Under Different Light Spectra

Recent developments in synthetic biology and metabolic engineering in microalgae towards biofuel production

Cas9-guide RNA ribonucleoprotein-induced genome editing in the industrial green alga Coccomyxa sp. strain KJ

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