Enhanced biodegradation of total petroleum hydrocarbons by implementing a novel two-step bioaugmentation strategy using indigenous bacterial consortium

Behera, Ipsita Dipamitra; Nayak, Manoranjan; Biswas, Subrata; Meikap, B.C.; Sen, Ramkrishna

doi:10.1016/j.jenvman.2021.112746

Cited by 39 publications

(14 citation statements)

References 36 publications

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“…4). The presence of TPH in the original PRS sample and its GC-FID chromatogram has been reported in our previous report (Behera et al 2021). From the Fig.…”

Section: Gc Analysissupporting

confidence: 72%

“…The hydrocarbon degrading bacterial cultures used in this study were previously isolated from the same PRS sample (Behera et al 2021). The four indigenous bacterial strains used for consortium are Dietzia lutea IRB191, Dietzia lutea IRB192, Staphylococcus warneri BSM19 and Stenotrophomonas pavanii IRB19 (Behera et al 2021). Equal volume of individual strains was mixed together at the end of the log phase to get the effective consortium (Behera et al 2021).…”

Section: Bacterial Inoculum and Maintenancementioning

confidence: 99%

“…The four indigenous bacterial strains used for consortium are Dietzia lutea IRB191, Dietzia lutea IRB192, Staphylococcus warneri BSM19 and Stenotrophomonas pavanii IRB19 (Behera et al 2021). Equal volume of individual strains was mixed together at the end of the log phase to get the effective consortium (Behera et al 2021). The indigenous isolated strains were preserved at -80 ℃ in sterile glycerol solution (20%, v/v).…”

Section: Bacterial Inoculum and Maintenancementioning

confidence: 99%

“…The indigenous isolated strains were preserved at -80 ℃ in sterile glycerol solution (20%, v/v). The optimum degradation strategy for efficient hydrocarbon degradation were pH, temperature, and percentage of oily sludge concentration were 7, 34 ℃, 2% (w/v) respectively (Behera et al 2021).…”

Section: Bacterial Inoculum and Maintenancementioning

confidence: 99%

“…However, 2% (w/v) of sludge was supplemented as the primary source of carbon and energy in each treatment flask. The initial pH of each medium was maintained at 7 and incubated at 34 ºC and 120 rpm (Behera et al 2021).…”

Section: Design Of Experimentsmentioning

confidence: 99%

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Performance Evaluation Of Bacterial Consortium For Biodegradation Of Total Petroleum Hydrocarbon: A Comparative Strategic Biostimulation Study

Behera

Meikap

Sen

2022

Preprint

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This research work demonstrated the comparative study of the efficacy of poultry litter extract (PLE) and inorganic fertilizer (NPK) as biostimulating agents for enhancing total petroleum hydrocarbon (TPH) degradation of petroleum refinery sludge (PRS) with bioaugmentation of an indigenously developed bacterial consortium. In this study, six sets of treatments such as natural attenuation, bioaugmentation with the indigenously developed bacterial consortium, and various biostimulation strategies with (MSM100, PLE100, NPK100, MSM50+PLE50, and MSM50+NPK50) were performed to meet 100% nutrient source for bacterial growth to enhance TPH degradation. Among all, the combined sources of MSM50+PLE50 showed the best performance by degrading the TPH up to 91.3 ± 4.1% within 28 d of the incubation period. The GC-FID analysis confirmed the efficacy of TPH degradation of PRS when PLE amendment with MSM. Further, the removal of maltene and asphaltene was also achieved 92 ± 3.7% and 52 ± 2.2% during this treatment. TPH degradation fitted to first-order kinetics with a rate constant 0.09 d-1 and half-life period of 7.7 d for MSM50+PLE50 amendment treatment along with bacterial consortium as bioaugmentation. This study revealed the implementation of the PLE amendment not only preferred as a nutrient source for bacterial growth but also enhanced TPH biodegradation in an eco-friendly strategic way by dropping the practice of inorganic salts.

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“…4). The presence of TPH in the original PRS sample and its GC-FID chromatogram has been reported in our previous report (Behera et al 2021). From the Fig.…”

Section: Gc Analysissupporting

confidence: 72%