Enhanced biodegradation of sugarcane bagasse by Clostridium thermocellum with surfactant addition

Cheng, Jingrong; Yu, Yang; Zhu, Ming‐Jun

doi:10.1039/c3gc42494d

Cited by 47 publications

(35 citation statements)

References 34 publications

(62 reference statements)

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“…The pH of those runs with a high inoculum size ( 10%) were lower than 5.0, indicating that cellulase in those systems was partly inactivated [11]. Our previous studies reported that a hydrogen production of 24.13 mmol/L was obtained with higher inoculation (10% C. thermocellum) and lower lignin-containing bagasse [21,27], indicating no significant difference with 7% inoculum size in this study. Decreases in pH during fermentation inhibit cellulose degradation, since pH affects the activity of the iron-containing cellulase enzyme [31].…”

Section: Effect Of Inoculum Size On H 2 Productioncontrasting

confidence: 65%

“…Therefore, we predicted the substrate structure and component could have great influence on hydrogen production and substrate utilization. A similar view has been proposed in another study, which stated that the complexity of the structure was the key factor affecting the substrate degradation rate [27]. Filter paper, a kind of relatively simple cellulosic biomass, is made of cotton fiber, while SCB, a natural complex lignocellulose, is mainly composed of cellulose, hemicellulose, and lignin.…”

Section: Characterization Of Degrading Cellulosic Biomasses By C Thementioning

confidence: 86%

“…Qing revealed that both hemicellulose and lignin inhibit the cellulose hydrolysis process [28]. A higher solid concentration brought in a higher lignin concentration, which increased the non-productive adsorption between lignin and cellulase [27]. Therefore, to relieve the mass transfer resistance and substrate inhibition, fed-batch fermentation perhaps makes sense, and it processes lignocellulose-hydrogen at a higher substrate concentration.…”

Section: Effect Of Scb Concentration On H 2 Productionmentioning

confidence: 97%

“…Filter paper, a kind of relatively simple cellulosic biomass, is made of cotton fiber, while SCB, a natural complex lignocellulose, is mainly composed of cellulose, hemicellulose, and lignin. Our previous study revealed the unproductive adsorption between cellulase and lignin in SCB [27]. Qing and Yang [28] demonstrated that xylan and various xylooligomers were more powerful inhibitors for cellulose hydrolysis than well-established glucose and cellobiose.…”

Section: Characterization Of Degrading Cellulosic Biomasses By C Thementioning

confidence: 98%

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Biohydrogen production from pretreated lignocellulose by Clostridium thermocellum

Cheng

Zhu

2016

Biotechnol Bioproc E

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In consolidated bioprocessing (CBP), the difference in optimum temperature between saccharification and fermentation poses a significant technical challenge to producing bioenergy efficiently with lignocellulose. The thermophilic anaerobic strain of Clostridium thermocellum has the potential to overcome this challenge if hydrolysis and fermentation is performed at an elevated temperature. However, this strain is sensitive to structure and components of lignocellulosic materials. To understand biohydrogen production from lignocellulosic materials, C. thermocellum was examined for biohydrogen production as well as bioconversion from different cellulosic materials (Avicel, filter paper and sugarcane bagasse (SCB)). We investigated hydrolysis-inhibitory effects of the cellulosic material types on the substrate degradation and biohydrogen production of C. thermocellum 27405. Within 168 h, the substrate degradation ratios of Avicel, filter paper, and SCB were 83.01, 51.78, and 42.19%, respectively. The substrate utilization and biohydrogen production of SCB reached 81 and 89.77% those of filter paper, respectively, indicating that SCB is a feasible substrate for biohydrogen production. Additionally, optimizing fermentation conditions can improve biohydrogen production, with the optimal conditions being an inoculum size of 7%, substrate concentration of 2%, particle size of 0.074 mm, and yeast extract concentration of 1%. This research provides important clues in relation to the low-cost conversion of renewable biomass to biohydrogen.

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Section: Effect Of Inoculum Size On H 2 Productioncontrasting

confidence: 65%

Section: Characterization Of Degrading Cellulosic Biomasses By C Thementioning

confidence: 86%

Section: Effect Of Scb Concentration On H 2 Productionmentioning

confidence: 97%

Section: Characterization Of Degrading Cellulosic Biomasses By C Thementioning

confidence: 98%

See 2 more Smart Citations

Biohydrogen production from pretreated lignocellulose by Clostridium thermocellum

Cheng

Zhu

2016

Biotechnol Bioproc E

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“…Furthermore, co-culture of C. thermocellum and Thermoanaerobacterium aotearoense can enhance the hydrogen production [1]. Besides, our studies showed that surfactant PEG8000 and CaCO3 both have positive effects on the hydrogen production by C. thermocellum [2][3][4].…”

mentioning

confidence: 64%

Biohydrogen Production from Waste Biomass

Zhu

2016

Tr Ren Energy

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Enhancing hemoglobin peptide production from chicken blood fermentation by food‐grade nonionic surfactant

Li¹,

Wu²,

Zhang³

et al. 2019

Biotech and App Biochem

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This study is focused on employing a potential process technology for enhancing hemoglobin peptides production from chicken blood. Effects of surfactants on chicken blood biodegradation and hemoglobin polypeptide accumulation were evaluated and the bioconversion conditions were optimized. Results suggested that surfactants exhibited the positive effect on hemoglobin peptides production during chicken blood bioconversion by Aspergillus niger. Dodecyl glucopyranoside was selected as the optimal surfactant and added at the 48th hour of the fermentation process (64 H) at the concentration of 6.0 g/L. Under the optimized conditions, 104.5 mg·N/mL amino nitrogen, 638.3 mg·N/mL nonprotein nitrogen, and 766.3 mg·N/mL soluble nitrogen were detected, which increased by approximately 0.7‐, 3.7‐, and 3.8‐fold, respectively, compared with the control. Furthermore, the acid protease stability was remarkably intensified and the accumulated peptides were mainly distributed at 500–2,000 Da. Results from this work corroborate the potential of applying dodecyl glucopyranoside in hemoglobin polypeptide production from chicken blood.

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Enhanced biodegradation of sugarcane bagasse by Clostridium thermocellum with surfactant addition

Abstract: SCB biodegradation and reducing sugar accumulation were remarkably increased by adding surfactant in the CBP system usingC. thermocellum.

Cited by 47 publications

References 34 publications

Biohydrogen production from pretreated lignocellulose by Clostridium thermocellum

Biohydrogen production from pretreated lignocellulose by Clostridium thermocellum

Biohydrogen Production from Waste Biomass

Enhancing hemoglobin peptide production from chicken blood fermentation by food‐grade nonionic surfactant

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