Enhanced biodegradation of sediment-bound heavily weathered crude oil with ligninolytic enzymes encapsulated in calcium-alginate beads

Kucharzyk, Katarzyna H.; Benotti, Mark J.; Darlington, Ramona; Lalgudi, Ramanathan S.

doi:10.1016/j.jhazmat.2018.06.036

Cited by 35 publications

(20 citation statements)

References 40 publications

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“…In the encapsulation process, the enzymes are partially damaged, and in the first studies on capsules with the enzyme, a high initial activity of the encapsulated enzyme was not achieved. Usually, the activity of encapsulated enzymes decreased by a factor of 6-7 [1,6,[10][11][12][13][14][15]. Close results to our experimental data presented in this study on encapsulated urease were obtained in [16][17][18][19].…”

Section: Issn 2224-5308supporting

confidence: 81%

Development of a Biosensor of Urea With the Application of Polymer Technologies for Blood and Urine Analysis

Saltanat¹,

Maria²,

Аппазов³

et al. 2018

Series of Biological and Medical

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Based on polymeric nanotechnologies, enzyme sensors and microreactors have been developedin the way, that they can determine urea in liquids. The technology of manufacturing an enzymatic biosensor does not differ significantly from the known technology of manufacturing microcapsules with an enzyme by the laer-by-laer method. This allows us, when constructing a biosensor, to use the information obtained on encapsulated enzymes by other authors. It is shown that urea biosensor is able to work for a long time (up to 2 months) without significant loss of enzyme activity. Polymer technology for manufacturing sensors is less laborious and expensive compared to other similar technologies. We propose to develop biosensor devices – urea analyzers with polymer enzyme chips for express diagnostics of biological fluids (blood, urine). One of the significant results of this work from our point of view is two factors. The first factor is the optimization of the conditions for the production of a functionally active enzyme immobilized in a polyelectrolyte coating, when the enzyme after the immobilization procedure shows an activity comparable to that of a freshly prepared free enzyme. Such a result will allow reducing the cost of enzymes when creating a sensitive layer of the developed urea analyzer. And the second factor is that the polymer coating with the enzyme is able to work not only as an enzyme electrode, but also as an enzyme microreactor, without decreasing the rate of signal registration after passing the catalytic urease-urea reaction.

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Section: Issn 2224-5308supporting

confidence: 81%

Development of a Biosensor of Urea With the Application of Polymer Technologies for Blood and Urine Analysis

Saltanat¹,

Maria²,

Аппазов³

et al. 2018

Series of Biological and Medical

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“…As enzimas podem ser produzidas por uma variedade de microrganismos na natureza, como bactérias, fungos, cianobactérias e algas, sendo capazes de degradar uma diversidade de contaminantes, são consideradas como macromoléculas orgânicas complexas que tem como principal função atuar como catalizadores orgânicos na degradação do contaminante (Kucharzyk, et al, 2018). Para ser utilizado na produção de enzimas, um microrganismo devem ser preferencialmente, seguro sob o ponto de vista biológico, possuindo status GRASgenerally recognized as safe, Food and Drug Administation (FDA), apresentar elevada capacidade de síntese e excreção da enzima, suportar condições ambientais adversas relacionadas com a pressão osmótica, a temperatura e a força iônica do meio, e ser tolerante à presença de substâncias tóxicas (Embrapa, 2015).…”

Section: Influência Das Enzimas Na Biorremediaçãounclassified

“…As enzimas são consideradas catalisadores biológicos que atuam controlando a velocidade das reações e sua principal importância no processo de biodegradação de petróleo é seu reaproveitamento, visto que elas não são consumidas no processo de aceleração das reações (Vigneswaran, Kandhavadivu & Ananthasubramanian, 2014). As enzimas são moléculas orgânicas complexas produzidas por microrganismos, como bactérias, fungos e algas e organismos superiores como plantas, elas atuam como biocatalizadores dos contaminantes, permitindo que o processo de biodegradação ocorra (Kucharzyk, et al, 2018;Wang, et al, 2021).…”

Section: Introductionunclassified

Processos enzimáticos na biorremediação e fitorremediação de petróleo em sedimentos de manguezal: uma revisão

Lacerda

Santana

Filho

et al. 2021

RSD

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Diversos vazamentos de petróleo em mares e oceanos ocorreram nas últimas décadas, afetando principalmente estuários e manguezais. Acidentes ambientais envolvendo o petróleo, seus derivados e os resíduos gerados têm sido uma preocupação crescente, por conta dos hidrocarbonetos do petróleo ser considerados contaminantes orgânicos tóxicos e de difícil degradação. Os avanços biotecnológicos têm levado os pesquisadores a adotar a biorremediação e fitorremediação como alternativas potencialmente eficazes para solos e sedimentos contaminados e o uso enzimas têm sido adotado como uma estratégia além do uso de plantas e microrganismos na biorremediação. Este estudo consiste em uma revisão bibliográfica baseada em livros, artigos, teses, dissertações e sites, com o intuito de reunir e avaliar os processos enzimáticos na fitorremediação e fitorremediação em ambientes impactados por atividades do petróleo.

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“…As a comparison, the use of biopellets in this study removed all 1500 mg/L in 22 days, showing rapid TPH removal when the biopellet was applied. Kucharzyk et al [18] applied ligninolytic enzymes encapsulated in beads to treat various types of crude oils. About 70% of THPs as crude oils were removed in 14 days, and the removal rate increased to~90% if weathered crude oil was treated.…”

Section: Biopellet Manufacturing and Performancesmentioning

confidence: 99%

Use of a Novel Biopellet to Treat Total Petroleum Hydrocarbon Contaminated Groundwater

Yang¹,

Chen²

2020

Water

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Conventional pump-and-treat strategies for dealing with groundwater contamination are both energy- and time-consuming. Potential passive biological techniques are of interest to remedy the massive volume of total petroleum hydrocarbon (TPH)-contaminated groundwater worldwide. In this study, novel biopellets made of TPH-acclimated microbes, fermented fruit peel materials, and CaO2 recycled from eggshells were manufactured to treat TPH-contaminated groundwater. The biopellets provided 56 mg of oxygen and achieved a C:N:P ratio by weight of 10:4:1. Moreover, each biopellet was capped with alginate to prolong its floating time in water to 25 days. The mimicked groundwater spiked with 500 mg/L diesel TPHs (TPHd) was treated using our novelly manufactured biopellets. After 8 days of treatment, results showed a 98.8% removal of spiked TPHd at a rate of 64.1 mg/L per day, with a microbial count that increased from nearly zero to 1.0 × 107 CFU/mL. The residual TPHd constituents were mainly C13–C18. Furthermore, microbial consumption of N, P, and oxygen was noted during the 8-day period of TPHd removal. As the TPHd level increased to 1500 mg/L, the removal rate reached 45 mg/L per day, and all TPHd had been removed after 22 days.

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Enhanced biodegradation of sediment-bound heavily weathered crude oil with ligninolytic enzymes encapsulated in calcium-alginate beads

Cited by 35 publications

References 40 publications

Development of a Biosensor of Urea With the Application of Polymer Technologies for Blood and Urine Analysis

Development of a Biosensor of Urea With the Application of Polymer Technologies for Blood and Urine Analysis

Processos enzimáticos na biorremediação e fitorremediação de petróleo em sedimentos de manguezal: uma revisão

Use of a Novel Biopellet to Treat Total Petroleum Hydrocarbon Contaminated Groundwater

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