Enhanced Biocompatibility for SAOS-2 Osteosarcoma Cells by Surface Coating with Hydrophobic Epoxy Resins

Abstract

Background and Aims: Implants for surgical needs are produced from different materials including metals, alloys, ceramics or polymers. Metal implants are preferred in those disciplines where sufficient mechanical strength is needed, including traumatology, orthopedic or dental surgery. Further, modern tissue engineering techniques require scaffold materials to generate shape and stability for in vitro generated transplants. However, the biocompatibility and surface contact of most implants or scaffold materials to vital bone or other tissues are not optimal. Therefore we investigated the biocompatibility of different polymer surfaces to an osteoblastic cell line as a function of wettability or hydrophobicity to describe some of the surface parameters influencing the cell to implant or cell to scaffold contact. Methods: Glass slides were coated with different polymers and in some cases physically or chemically modified. SAOS-2 osteosarcoma cells were used for the biocompatibility tests on 16 different polymers and modifications thereof. The viability of the adherent cells was investigated by MTT assay. Commercially available tissue culture vessels served as controls. Results: We report that excellent biocompatibility to SAOS-2 osteoblastic cells can be obtained with hydrophobic surfaces generated for instance by epoxy resins. Chemical modification of epoxy resin surfaces yielded even a further increased viability index surpassing the viability index obtained with cell culture vessels. Conclusion: We conclude that modified hydrophobic surfaces represent an interesting group of compounds for coating endoprosthetic implants or scaffolds for the purposes of tissue engineering.

Biocompatibility of oxygen-plasma-treated polystyrene substrates

“…A high surface energy often results in a good adhesion of different materials onto the substrate. Although, many works have been published on surface functionalization of polymer materials, much less work has been devoted to proliferation of human cells on modified polymers [14][15][16][17][18][19][20][21]. The scope of the present paper touches the proliferation of HMEC cells on plasma modified polystyrene.…”

“…A popular method for improvement of polymer biocompatibility is treatment by nonequilibrium gaseous plasma [4][5][6][7][8]. Depending on particular requirements, a variety of different plasmas can be applied for surface functionalization.…”

Epoxy networks for medicine applications: Mechanical properties and in vitro biological properties

“…[15][16][17][18] The majorities of these studies are based on histological observations and morphologic evaluations and are directed toward understanding the cellular organization immediately adjacent to the implant. Our efforts over the past several years have been directed toward developing a better understanding of the relationships between polymer structure and blood compatibility.…”

“…Although numerous studies have been published about the biocompatibility property of diepoxy compounds and epoxy polymers, [15][16][17][18] to our knowledge, no work has been undertaken about the blood compatibility of epoxies materials based on epoxy-aliphatic amines systems.…”

Redes epóxi/amina alifáticas com perspectivas para aplicações cardiovasculares. Propriedades biológicas in vitro

“…As propriedades de biocompatibilidade e citotoxicidade de monômeros epoxídicos e de polímeros epoxídicos [14][15][16][17][18] tem sido relatadas, mas até a presente data, não foram observadas publicações a respeito da utilização destes polímeros para aplicações cardiovasculares. Recentemente, nossos estudos sobre a hemocompatibilidade de polímeros epoxídicos, demonstram que esses materiais possuem boas propriedades de antitrombogenicidade e adequada ausência de citotoxicidade sugerindo que tais materiais possuem propriedades biológicas favoráveis para aplicação como revestimentos de dispositivos cardiovasculares [14].…”