Enhanced Binding of Phenosafranin to Triblock Copolymer F127 Induced by Sodium Dodecyl Sulfate: A Mixed Micellar System as an Efficient Drug Delivery Vehicle

Abstract

In this study, we explored the interaction of a cationic phenazinium dye, phenosafranin (PSF, here used as a model drug), with pluronic block copolymer F127, both in the presence and in the absence of the anionic surfactant sodium dodecyl sulfate (SDS), which forms mixed micelles with F127. We applied both steady-state and time-resolved spectroscopic techniques, along with isothermal titration calorimetry (ITC), to demonstrate the binding of the probe PSF to both the pluronic and F127/SDS mixed micelles. Dynamic light scattering (DLS) study revealed that, upon interaction with SDS, the hydrodynamic diameter (dH) of F127 micelles decreased due to the formation of the mixed micelles. The PSF penetrated to the more hydrophobic interior of the mixed micellar system as compared to F127 micelles alone. Micropolarity and fluorescence-quenching experiments revealed that PSF is more deeply seated in the case of F127/SDS mixed micelles. Through a partition coefficient, lifetime measurements, and time-resolved anisotropy experiments, we also established that the partitioning of the probe within the F127 micelles in the presence of SDS is almost double than that in its absence. ITC data corroborates the fact that the binding of PSF is the strongest and most thermodynamically favorable when mixed micelles are formed, which enables our system to serve as an excellent drug delivery vehicle when compared to F127 alone.

A Global Scenario on the Dynamics of Excited State Proton Transfer of Pyranine in the Mixed Micellar Assemblies: Role of Water Accessibility in the Probe Location+

“…The diagrams of light scattering intensity against particle size for the neat F127 micelles and the mixed systems have been shown in Figure a. The hydrodynamic radius of F127 micelles was found to be ∼13 nm, which is well supported by the finding of previous reports . The scattering intensity as well as hydrodynamic radius (7.0 nm for n =0.5, and 5.9 nm for n =1.25) gradually decreased upon formation of F127‐SDS mixed micellar system.…”

“…Hence, all SDS molecules should then remain as monomers. Here we have maintained a low ‘n’ value to prepare only mixed micelles so that any other smaller aggregate formation or breaking down of the F127 micelles does not appear . The diagrams of light scattering intensity against particle size for the neat F127 micelles and the mixed systems have been shown in Figure a.…”

Study on surface properties of sodiumdodecyl sulfate and dodecyltrimethylammonium bromide mixed surfactants and their interaction with dyes

“…Earlier researchers have been analyzed the effect of dyes with mixed surfactants in the aqueous and non-aqueous solvent medium [34, 35, 36, 37, 38, 39, 40]. Samiey et al.…”

“…Earlier researchers have been analyzed the effect of dyes with mixed surfactants in the aqueous and non-aqueous solvent medium [34,35,36,37,38,39,40]. Samiey et al [41] have been explained the rate constant of alkaline fading of crystal violet was measured in the presence of non-ionic (TX-100), cationic (DTAB) and anionic (SDS) surfactants at 283e303 K. Dey et al [42] have been reported the fluorescence correlation spectroscopic study of dyes with SDS and DTAB.…”

Binary systems of Brij® surfactants with Pluronic® F127 as griseofulvin carrier

“…The mixtures F127/Brij 700 show an opposite behaviour of F127/Brij 78 and F127/ Brij 98 mixtures as the F127 concentration increases (Figure 3). In the F127/Brij 700 mixtures, the hydrodynamic diameter of particles decreases by increasing concentration of F127, as in the systems formed by mixed micelles obtained of the mixture F127/ SDS in the study proposed by Mondal et al 19 Pellosi et al 20 observed in their studies of pluronic mixed micelles that, in mixtures containing F127/ P123 there was an inverse behaviour: the hydrodynamic diameter of particles increases when the concentration of F127 decreases.…”