Enhanced Bacterial Uptake and Bactericidal Properties of Ofloxacin Loaded on Bioadhesive Hydrogels AgainstPseudomonas aeruginosa

Abstract

Carbomer hydrogels 971pNf, 934pNf and 940Nf loaded with ofloxacin were characterized and their antimicrobial properties evaluated. bactericidal profiles show improved efficacy and prolonged activity exhibited by ofloxacin-containing hydrogels against Pseudomonas aeruginosa. Analysis of bactericidal index (BI) values after a short time of drug exposure confirms the higher potency of hydrogels compared with that of ofloxacin. Increased BI values observed after 24 h indicate prolonged action against the microorganisms evaluated. The bacterial uptake of ofloxacin from hydrogels was higher than that obtained with a solution of free ofloxacin in both fluoroquinolone-sensitive and -resistant P. aeruginosa. The improved uptake in fluoroquinolone-resistant isolates was correlated with the viscosity of hydrogels. The performance of hydrogels seems to be related to their bioadhesive properties that allow prolonged contact time and the release of an effective amount of drug close to bacterial cells. Hence, hydrogels could be used in the development of more effective formulations for topical administration of antibiotics. Improved performance of an old antibiotic can preserve the use of new generation fluoroquinolones.

PEGylated ofloxacin nanoparticles render strong antibacterial activity against many clinically important human pathogens

“…3C and 4). Accordingly, enhanced bacterial uptake of OFX loaded bioadhesive hydrogels or OFX-containing Eudragit E100 ® dispersions were reported to increase bactericidal properties against P. aeruginosa [29,30]. In the present study, OFX-mPEG-PLGA nanoparticles consistently showed better bactericidal activity against all the tested pathogenic bacterial strains, as revealed by the severe reduction in the number of colonies than the free drug or OFX-PLGA nanoparticles ( Fig.…”

Eudragit E100® potentiates the bactericidal action of ofloxacin against fluoroquinolone-resistant Pseudomonas aeruginosa

“…Strategies to minimize the low OM‐permeability of P. aeruginosa could significantly contribute to the success of therapy for infections with this organism. Several reports describe the effect of cationic peptides as antimicrobial agents or diverse agents as detergents or polymers used in attempts to alter the OM‐permeability of Gram‐negative bacteria (Mugabe et al ., ; Dillen et al ., ; Tin et al ., ; Romero et al ., ).…”

Identification of Functions Affecting Predator-Prey Interactions between Myxococcus xanthus and Bacillus subtilis

“…They can act as defensive molecules shaping microbial communities by killing or by inhibiting growth (2,19). However, many recent publications show that the role of specialized metabolites may be more complex, as they can induce biofilm formation and act as signaling molecules (47,48). Therefore, it was not surprising that we identified a transposon insertion mutant in the M. xanthus hsf gene cluster known to regulate specialized metabolite production (38).…”