Enhanced B-phycoerythrin production by the red microalga Porphyridium marinum: A powerful agent in industrial applications

Gargouch, Nesrine; Karkouch, Ines; Elleuch, Jihen; Elkahoui, Salem; Michaud, Phillipe; Abdelkafi, Slim; Laroche, Céline; Fendri, Imen

doi:10.1016/j.ijbiomac.2018.09.037

Cited by 43 publications

(30 citation statements)

References 46 publications

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“…On the other hand, higher levels of light intensity (100 to 140 µmol m −2 s −1 ) yielded biomass production above 2 g L −1 [26,39]. A study with Porphyridium marinum was able to produce 2 g L −1 with a light intensity of 150 µmol m −2 s −1 to later change the condition to 70 µmol m −2 s −1 [40]. The trends in the present study and in the literature mentioned above indicate that high biomass production occurs at a high light intensity.…”

Section: Discussionmentioning

confidence: 99%

Light Intensity and Nitrogen Concentration Impact on the Biomass and Phycoerythrin Production by Porphyridium purpureum

et al. 2019

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Several factors have the potential to influence microalgae growth. In the present study, nitrogen concentration and light intensity were evaluated in order to obtain high biomass production and high phycoerythrin accumulation from Porphyridium purpureum. The range of nitrogen concentrations evaluated in the culture medium was 0.075–0.450 g L−1 and light intensities ranged between 30 and 100 μmol m−2 s−1. Surprisingly, low nitrogen concentration and high light intensity resulted in high biomass yield and phycoerythrin accumulation. Thus, the best biomass productivity (0.386 g L−1 d−1) and biomass yield (5.403 g L−1) were achieved with NaNO3 at 0.075 g L−1 and 100 μmol m−2 s−1. In addition, phycoerythrin production was improved to obtain a concentration of 14.66 mg L−1 (2.71 mg g−1 of phycoerythrin over dry weight). The results of the present study indicate that it is possible to significantly improve biomass and pigment production in Porphyridium purpureum by limiting nitrogen concentration and light intensity.

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Section: Discussionmentioning

confidence: 99%

Light Intensity and Nitrogen Concentration Impact on the Biomass and Phycoerythrin Production by Porphyridium purpureum

et al. 2019

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“…% inhibition = [100 − ((A Sample − A blank )/(A Control − A blank ))] × 100 (1) with A Blank and A Control corresponding to the absorbance of RPMI medium and untreated biofilm at 630 nm, respectively.…”

Section: Inhibition Of Biofilm Formationmentioning

confidence: 99%

“…In recent years, several studies have been conducted on bioactive molecules extracted from microalgal strains such as carbohydrate polymers, proteins, lipids and pigments whose biological and physicochemical properties can be used in the food, cosmetics, medical and pharmacological industries [1,2]. Among these microalgae, red microalgae, especially the genus Porphyridium and Rhodella, have attracted interest for their richness in sulfated exopolysaccharides (EPS).…”

Section: Introductionmentioning

confidence: 99%

Potential of Exopolysaccharide from Porphyridium marinum to Contend with Bacterial Proliferation, Biofilm Formation, and Breast Cancer

et al. 2021

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Exopolysaccharide (EPS) from marine microalgae are promising sources of a new generation of drugs. However, lot of them remain to be discovered and tested. In this study, EPS produced by Porphyridium marinum and its oligomers prepared by High Pressure Homogenizer have been tested for different biological activities, i.e., antibacterial, anti-fungal and antibiofilm activities on Candida albicans, as well as for their effects on the viability of murine breast cancer cells. Results have shown that all EPS samples present some biological activity. For antibacterial and antibiofilm activities, the native EPS exhibited a better efficiency with Minimum Inhibitory Concentration (MIC) from 62.5 µg/mL to 1000 µg/mL depending on the bacterial strain. For Candida albicans, the biofilm formation was reduced by about 90% by using only a 31.3 µg/mL concentration. Concerning breast cancer cells, lower molar masses fractions appeared to be more efficient, with a reduction of viability of up to 55%. Finally, analyses of polymers composition and viscosity measurements were conducted on all samples, in order to propose hypotheses involving the activities caused by the intrinsic properties of polymers.

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“…This pigment is used as fluorescent markers in biomedical research. It also has applicability in medical and cosmetic industries as a natural colorant and active compound since it has antioxidant [ 58 ], antimicrobial, and anticancer activity [ 57 ]. However, the extract produced in this study showed excellent potential as an additive and functional ingredient of food due to its nature, unique color, and biological activity because of its food-grade status.…”

Section: Resultsmentioning

confidence: 99%

Implementation of kLa-Based Strategy for Scaling Up Porphyridium purpureum (Red Marine Microalga) to Produce High-Value Phycoerythrin, Fatty Acids, and Proteins

et al. 2021

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Porphyridium purpureum is a well-known Rhodophyta that recently has attracted enormous attention because of its capacity to produce many high-value metabolites such as the pigment phycoerythrin and several high-value fatty acids. Phycoerythrin is a fluorescent red protein-pigment commercially relevant with antioxidant, antimicrobial activity, and fluorescent properties. The volumetric mass transfer coefficient (kLa) was kept constant within the different scaling-up stages in the present study. This scaling-up strategy was sought to maintain phycoerythrin production and other high-value metabolites by Porphyridium purpureum, using hanging-bag photobioreactors. The kLa was monitored to ensure the appropriate mixing and CO2 diffusion in the entire culture during the scaling process (16, 80, and 400 L). Then, biomass concentration, proteins, fatty acids, carbohydrates, and phycoerythrin were determined in each step of the scaling-up process. The kLa at 16 L reached a level of 0.0052 s−1, while at 80 L, a value of 0.0024 s−1 was achieved. This work result indicated that at 400 L, 1.22 g L−1 of biomass was obtained, and total carbohydrates (117.24 mg L−1), proteins (240.63 mg L−1), and lipids (17.75% DW) were accumulated. Regarding fatty acids production, 46.03% palmitic, 8.03% linoleic, 22.67% arachidonic, and 2.55% eicosapentaenoic acid were identified, principally. The phycoerythrin production was 20.88 mg L−1 with a purity of 2.75, making it viable for food-related applications. The results of these experiments provide insight into the high-scale production of phycoerythrin via the cultivation of P. purpureum in an inexpensive and straightforward culture system.

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Enhanced B-phycoerythrin production by the red microalga Porphyridium marinum: A powerful agent in industrial applications

Cited by 43 publications

References 46 publications

Light Intensity and Nitrogen Concentration Impact on the Biomass and Phycoerythrin Production by Porphyridium purpureum

Light Intensity and Nitrogen Concentration Impact on the Biomass and Phycoerythrin Production by Porphyridium purpureum

Potential of Exopolysaccharide from Porphyridium marinum to Contend with Bacterial Proliferation, Biofilm Formation, and Breast Cancer

Implementation of kLa-Based Strategy for Scaling Up Porphyridium purpureum (Red Marine Microalga) to Produce High-Value Phycoerythrin, Fatty Acids, and Proteins

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