Enhanced application of square wave voltammetry with glassy carbon electrode coupled to multivariate calibration tools for the determination of B6 and B12 vitamins in pharmaceutical preparations

Hernández, Silvia R.

doi:10.1016/s0039-9140(03)00335-7

Cited by 75 publications

(29 citation statements)

References 43 publications

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“…There are a great variety of analytical methods based on fluorimetric [13], spectrophotometric [14,15], or electrochemical [16,17] monitoring the overall quantification of a given B vitamin. However, these methods do not offer the possibility of individual quantification of each vitamer, for which a separation step previous to the determination step is mandatory.…”

Section: Introductionmentioning

confidence: 99%

Determination of B2 and B6 vitamers in serum by capillary electrophoresis-molecular fluorescence-charge coupled detector

Priego-Capote

Castro

2005

Electrophoresis

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A method for the separation by capillary zone electrophoresis and determination of vitamers of two important water-soluble vitamins, i.e., B2 and B6, is proposed here. The working conditions for optimal separation were obtained by a multivariate methodology in order to succeed in the best resolution in the shortest analysis time. The optimization of the buffer composition together with other variables, such as analysis and injection voltage, temperature of the capillary cassette, and injection time, resulted in a solution of 30 mM KH2PO4 adjusted to pH 8.5 with formic acid. Concerning the detection step, the target analytes were quantified by molecular fluorescence, for which two different detectors, a photomultiplier tube (PMT) and a charge-coupled detector (CCD), were compared in terms of resolution, sensitivity, and precision. A Xe-Hg lamp was used as an irradiation source in the two cases. The best option was the CCD, which provides three-dimensional electropherograms and enables to solve the overlapped peaks. Besides, the sensitivity of the CCD was similar to that of the PMT, due to the treatment data, obtaining limits of detection and quantification from 1.16 to 27.1 ng/g and from 3.83 to 89.4 ng/g, respectively. The method was applied to the serum samples for which a prior liquid-liquid extraction using ethanol in an acid medium was mandatory for eliminating the interferences and concentrating the analytes by a factor of 5. The rapidity of the analysis (13 min for the electrophoretic separation) and the excellent precision (repeatability and within-laboratory reproducibility between 2.86 and 4.11% and 7.03 and 8.45%, respectively, both expressed as relative standard deviation) demonstrated the capability of the proposed method for a clinical routine analysis.

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Section: Introductionmentioning

confidence: 99%

Determination of B2 and B6 vitamers in serum by capillary electrophoresis-molecular fluorescence-charge coupled detector

Priego-Capote

Castro

2005

Electrophoresis

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“…As it can be seen from Table 2, the linear range of the proposed detection of B 12 is competitive to the reported ones. Furthermore, the calculated limit of detection of the proposed methodology is lower compared to those reported on references [4,[28][29][30][31]. In addition, the detection limit of the proposed methodology, comprising the modified CPE with (A) and dsDNA, is lower than that of the unmodified CPE [27].…”

Section: Discussionmentioning

confidence: 65%

“…In specific, vitamin B 12 or cobalamin is an organometallic cofactor with a complex structure [1,2]. Vitamin B 12 exhibits a rich redox chemistry centered on the cobalt atom, where the Co III in the vitamin B 12 can be reduced reversibly to Co II and further reduced to Co I [3,4]. Vitamin B 12 adsorbs on electrode surfaces and can be used for oxidation or reduction reactions [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Development of an electrochemical DNA biosensor for the detection of vitamin B12 (cyanocobalamin) at a carbon paste modified electrode with a manganese(II) complex

Dimitropoulou¹,

Karastogianni²,

Girousi³

2017

J Appl Bioanal

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“…This finding is not surprising, considering the complexity of pyridoxine multi-step/multi-electron reactions that could take place. 23,42,43 The process is also pH-dependent due to complex distribution of species resulting from acid-base and hydration equilibria. Since redox behavior of all other vitamins is also strongly dependent on the solution pH, preliminary experiments were performed to choose the best medium.…”

Section: Resultsmentioning

confidence: 99%

Electrochemical signatures of multivitamin mixtures

Mohan

Brunetti

Bulbarello

et al. 2015

Analyst

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Enhanced application of square wave voltammetry with glassy carbon electrode coupled to multivariate calibration tools for the determination of B6 and B12 vitamins in pharmaceutical preparations

Cited by 75 publications

References 43 publications

Determination of B2 and B6 vitamers in serum by capillary electrophoresis-molecular fluorescence-charge coupled detector

Determination of B2 and B6 vitamers in serum by capillary electrophoresis-molecular fluorescence-charge coupled detector

Development of an electrochemical DNA biosensor for the detection of vitamin B12 (cyanocobalamin) at a carbon paste modified electrode with a manganese(II) complex

Electrochemical signatures of multivitamin mixtures

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