Enhanced apoptotic and anticancer potential of paclitaxel loaded biodegradable nanoparticles based on chitosan

Gupta, Umesh; Sharma, Saurabh; Khan, Iliyas; Gothwal, Avinash; Sharma, Ashok; Singh, Yuvraj; Chourasia, Manish K.; Kumar, Vipin

doi:10.1016/j.ijbiomac.2017.02.030

Cited by 74 publications

(24 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All of these nanocarriers are formulated for natural product based drug delivery. For applications in cancer treatment, Gupta et al [ 231 ] synthesized chitosan based nanoparticles loaded with Paclitaxel (Taxol) derived from Taxus brevifolia , and utilized them for treatment of different kinds of cancer. The authors concluded that the nanoparticle loaded drug exhibited better activity with sustained release, high cell uptake and reduced hemolytic toxicity compared with pure Paclitaxel [ 231 ].…”

Section: Natural Product-based Nanotechnology and Drug Deliverymentioning

confidence: 99%

Nano based drug delivery systems: recent developments and future prospects

et al. 2018

View full text Add to dashboard Cite

Nanomedicine and nano delivery systems are a relatively new but rapidly developing science where materials in the nanoscale range are employed to serve as means of diagnostic tools or to deliver therapeutic agents to specific targeted sites in a controlled manner. Nanotechnology offers multiple benefits in treating chronic human diseases by site-specific, and target-oriented delivery of precise medicines. Recently, there are a number of outstanding applications of the nanomedicine (chemotherapeutic agents, biological agents, immunotherapeutic agents etc.) in the treatment of various diseases. The current review, presents an updated summary of recent advances in the field of nanomedicines and nano based drug delivery systems through comprehensive scrutiny of the discovery and application of nanomaterials in improving both the efficacy of novel and old drugs (e.g., natural products) and selective diagnosis through disease marker molecules. The opportunities and challenges of nanomedicines in drug delivery from synthetic/natural sources to their clinical applications are also discussed. In addition, we have included information regarding the trends and perspectives in nanomedicine area.

show abstract

Section: Natural Product-based Nanotechnology and Drug Deliverymentioning

confidence: 99%

Nano based drug delivery systems: recent developments and future prospects

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Moreover, the abundant active amine and hydroxyl groups in CS could offer many opportunities for chemical modification. In recent years, several chitosan-based PTX delivery systems have been developed, such as N -mercapto acetyl- N ′-octyl- O , N ′′-glycol chitosan micelles [ 13 ], 3,6- O , O ′-dimyristoyl chitosan micelles [ 14 ], folic acid–cholesterol–chitosan micelles [ 15 ], PTX conjugated trimethyl chitosan nanoparticles [ 16 ], palmitoyl chitosan nanoparticles [ 17 ], N -succinyl-chitosan nanoparticles [ 18 ] and PTX-loaded chitosan nanoparticles prepared by the nano-emulsion method [ 19 ]. However, chitosan with high molecular weight has poor solubility in aqueous medium at neutral pH, which limits its medical and pharmaceutical applications.…”

Section: Introductionmentioning

confidence: 99%

Amphiphilic Polymeric Micelles Based on Deoxycholic Acid and Folic Acid Modified Chitosan for the Delivery of Paclitaxel

Liang

Wang

et al. 2018

IJMS

View full text Add to dashboard Cite

The present investigation aimed to develop a tumor-targeting drug delivery system for paclitaxel (PTX). The hydrophobic deoxycholic acid (DA) and active targeting ligand folic acid (FA) were used to modify water-soluble chitosan (CS). As an amphiphilic polymer, the conjugate FA-CS-DA was synthesized and characterized by Proton nuclear magnetic resonance (1H-NMR) and Fourier-transform infrared spectroscopy (FTIR) analysis. The degree of substitutions of DA and FA were calculated as 15.8% and 8.0%, respectively. In aqueous medium, the conjugate could self-assemble into micelles with the critical micelle concentration of 6.6 × 10−3 mg/mL. Under a transmission electron microscope (TEM), the PTX-loaded micelles exhibited a spherical shape. The particle size determined by dynamic light scattering was 126 nm, and the zeta potential was +19.3 mV. The drug loading efficiency and entrapment efficiency were 9.1% and 81.2%, respectively. X-Ray Diffraction (XRD) analysis showed that the PTX was encapsulated in the micelles in a molecular or amorphous state. In vitro and in vivo antitumor evaluations demonstrated the excellent antitumor activity of PTX-loaded micelles. It was suggested that FA-CS-DA was a safe and effective carrier for the intravenous delivery of paclitaxel.

show abstract

“…Owing to its unique physical and chemical properties and biological functions, chitosan has been one of the most fascinating biopolymers for antitumor drugs [29]. Researches showed that chitosan could act on tumor cells directly to interfere with cell metabolism, inhibit cell growth, or induce cell apoptosis [30]. As Michela et al [31] demonstrated that marine diatom cocconeis scutellum and eicosapentaenoic acid (EPA) contributed to proliferation inhibition and apoptosis of BT-20 cells in vitro.…”

Section: Introductionmentioning

confidence: 99%

Seleno-short-chain chitosan induces apoptosis in human breast cancer cells through mitochondrial apoptosis pathway in vitro

Zhao

et al. 2018

Cell Cycle

View full text Add to dashboard Cite

Seleno-short-chain chitosan (SSCC) was a synthesized chitosan derivative with the molecular weight of 4826.986 Da. The study is aimed to investigate cytotoxicity of SSCC on human breast cancer MCF-7 and BT-20 cells and explore apoptosis-related mechanism in vitro. The MTT (3- [4,5-Dimethylthiazol-2-yl]-2, 5-diphenylterazolium bromide) assay showed that SSCC exhibited significantly cytotoxic effects on MCF-7 and BT-20 cells in a dose- and time-dependent manner, and the effective inhibitory concentration was 100 μg/ml and 200 μg/ml, respectively. Apoptosis assay of these two kinds of cells was determined by Hoechst 33,342/PI and Annexin V-FITC/PI double staining. The cell cycle assay showed that SSCC triggered S and G2/M phase cell cycle arrest in MCF-7 cells and S phase cell cycle arrest in BT-20 cells in a time-dependent manner. Further studies demonstrated that SSCC led to the generation of reactive oxygen species (ROS) and the disruption of mitochondrial membrane potential (MMP) in these two kinds of cells. N- acetyl-L cysteine (NAC), as a radical scavenger, significantly inhibited the generation of ROS and decreased the apoptosis of MCF-7 and BT-20 cells. Moreover, the expression of mitochondrial apoptosis-related proteins was detected by western blot assay. SSCC up-regulated the expression of Bax, down-regulated the expression of Bcl-2, subsequently increased the release of cytochrome c from mitochondria to cytoplasm, and activated the cleavage of caspase-9 and -3, which finally induced apoptosis in MCF-7 and BT-20 cells in vitro. Consequently, these data indicated that SSCC could induce apoptosis of MCF-7and BT-20 cells in vitro by mitochondrial pathway.

show abstract

Enhanced apoptotic and anticancer potential of paclitaxel loaded biodegradable nanoparticles based on chitosan

Cited by 74 publications

References 35 publications

Nano based drug delivery systems: recent developments and future prospects

Nano based drug delivery systems: recent developments and future prospects

Amphiphilic Polymeric Micelles Based on Deoxycholic Acid and Folic Acid Modified Chitosan for the Delivery of Paclitaxel

Seleno-short-chain chitosan induces apoptosis in human breast cancer cells through mitochondrial apoptosis pathway in vitro

Contact Info

Product

Resources

About