Enhanced anticancer activity of encapsulated geraniol into biocompatible lipid nanoparticles against A549 human lung cancer cells

Rodenak-Kladniew, Boris; Gambaro, Rocío Celeste; Cisneros, José Sebastián; Huck‐Iriart, Cristián; Padula, Gisel; Castro, Guillermo R.; Chain, Cecilia Yamil; Islan, Germán Abel

doi:10.1016/j.jddst.2023.104159

Cited by 14 publications

(10 citation statements)

References 43 publications

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“…CnEO release was determined as previously described [ 21 ] using two different buffer systems: phosphate buffer (pH 7.4) and acetate/acetic acid buffer (AC- + AcH, pH 5.0). The CnEO concentration was measured at λ max = 258 nm using a UV–Vis spectrophotometer (Shimadzu, Japan).…”

Section: Methodsmentioning

confidence: 99%

“…SLNs are biocompatible, non-toxic, and easily produced on a large scale [ 19 ]. Moreover, the inclusion of liquid lipids (such as EO) into the solid lipid matrix of SLN promotes their reorganization into nanostructured lipid carriers (NLC) [ 20 , 21 ], improving their stability, encapsulation efficiency, and drug loading capacity, and limiting the expulsion of cargo molecules during storage [ 17 , 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

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Cytotoxic Screening and Enhanced Anticancer Activity of Lippia alba and Clinopodium nepeta Essential Oils-Loaded Biocompatible Lipid Nanoparticles against Lung and Colon Cancer Cells

et al. 2023

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Plant and herbal essential oils (EOs) offer a wide range of pharmacological actions that include anticancer effects. Here, we evaluated the cytotoxic activity of EO from Lippia alba (chemotype linalool), L. alba (chemotype dihydrocarvone, LaDEO), Clinopodium nepeta (L.) Kuntze (CnEO), Eucalyptus globulus, Origanum × paniculatum, Mentha × piperita, Mentha arvensis L., and Rosmarinus officinalis L. against human lung (A549) and colon (HCT-116) cancer cells. The cells were treated with increasing EO concentrations (0–500 µL/L) for 24 h, and cytotoxic activity was assessed. LaDEO and CnEO were the most potent EOs evaluated (IC50 range, 145–275 µL/L). The gas chromatography–mass spectrometry method was used to determine their composition. Considering EO limitations as therapeutic agents (poor water solubility, volatilization, and oxidation), we evaluated whether LaDEO and CnEO encapsulation into solid lipid nanoparticles (SLN/EO) enhanced their anticancer activity. Highly stable spherical SLN/LaDEO and SLN/CnEO SLN/EO were obtained, with a mean diameter of 140–150 nm, narrow size dispersion, and Z potential around −5mV. EO encapsulation strongly increased their anticancer activity, particularly in A549 cells exposed to SLN/CnEO (IC50 = 66 µL/L CnEO). The physicochemical characterization, biosafety, and anticancer mechanisms of SLN/CnEO were also evaluated in A549 cells. SLN/CnEO containing 97 ± 1% CnEO was highly stable for up to 6 months. An increased in vitro CnEO release from SLN at an acidic pH (endolysosomal compartment) was observed. SLN/CnEO proved to be safe against blood components and non-toxic for normal WI-38 cells at therapeutic concentrations. SLN/CnEO substantially enhanced A549 cell death and cell migration inhibition compared with free CnEO.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cytotoxic Screening and Enhanced Anticancer Activity of Lippia alba and Clinopodium nepeta Essential Oils-Loaded Biocompatible Lipid Nanoparticles against Lung and Colon Cancer Cells

et al. 2023

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“…The results demonstrated enhanced cytotoxicity in A549 lung cancer cells, with significant increases in cell death and inhibition of cell migration. These findings highlighted the potential of NLCs to enhance the therapeutic properties of geraniol, making it a more effective agent against lung cancer [73].…”

Section: Terpenoids and Terpenesmentioning

confidence: 86%

“…Enhanced Efficacy with Specificity: LNPs like biotin-decorated sunitinib-loaded carriers and transferrin-modified systems have increased drug delivery specificity and cellular uptake, significantly improving therapeutic efficacy [5,9,41,47,49,57,[73][74][75]83,84,89].…”

Section: Reduced Toxicity and Side Effectsmentioning

confidence: 99%

Lipid Nanoparticles in Lung Cancer Therapy

Omidian,

Gill,

Cubeddu

2024

Pharmaceutics

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This manuscript explores the use of lipid nanoparticles (LNPs) in addressing the pivotal challenges of lung cancer treatment, including drug delivery inefficacy and multi-drug resistance. LNPs have significantly advanced targeted therapy by improving the precision and reducing the systemic toxicity of chemotherapeutics such as doxorubicin and paclitaxel. This manuscript details the design and benefits of various LNP systems, including solid lipid–polymer hybrids, which offer controlled release and enhanced drug encapsulation. Despite achievements in reducing tumor size and enhancing survival, challenges such as manufacturing complexity, biocompatibility, and variable clinical outcomes persist. Future directions are aimed at refining targeting capabilities, expanding combinatorial therapies, and integrating advanced manufacturing techniques to tailor treatments to individual patient profiles, thus promising to transform lung cancer therapy through interdisciplinary collaboration and regulatory innovation.

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“…Huperzine A selective inhibitor of acetylcholinesterase NLC [356] Carthamus tinctorius antibacterial, anitoxidant, analgesic, sedative, anticonvulsant, immunosuppressive, anti-inflammatory NLC [357] Zingiber Officinale anti-inflammatory, antibacterial, hepatoprotective, anti-carcinogenic, anti-allergenic SLN [358] Anethol antifungal, antiaflatoxigenic, antioxidant NE [359] Allicin anti-inflammatory, antioxidant, neuroprotective Liposome [360] Capsaicin local anaesthetic Liposome [361] 17-hydroxy-jolkinolide B anti-carcinogenic Liposome [362] Kaempferol anti-carcinogenicr, anti-inflammatory, antioxidant NLC [363] Chamomile oil antibacterial, antifungal, anti-inflammatory, antispasmodic, anti-ulcer, antiviral, sedative effect SLN [364] Morin hydrate anti-carcinogenicr SLN [365] Geraniol anti-carcinogenic NLC [366] Eucalyptus oil antibacterial, antifungal, antiseptic, antihyperglycemic, antioxidant NLC [367] Gallic acid anti-cytotoxic, antiproliferative SLN [368] Linalool antiproliferative agent SLN, Liposome [369,370] Ginkgolides A and B anti-cardiovascular, cerebrovascular SLN [371] Hibiscus rosa sinensis antidepressant, aphrodisiac, abortifacient SLN [372] Amaranth oil anti-tumor, antimicrobial, antifungal NE [373] Avocado oil hypercholesterolemia NE [374] Nigella sativa antioxidative, antimicrobial SLN [375] Linseed oil anti-inflammatory, antitumorigenic NE, Liposome [376,377] Neem oil anti-hemorrhoid, antiprotozoal, antibacterial, antitoxoplasma activity SLN, NE [378,379] Glycyrrhizic acid anti-inflammation, antiviral, detoxification, hepatic protection modified LNP [380] Allium sativum (garlic) has been used widely all over the world as a food and a medicine for four millennia. It is a potential nutraceutical that has been used to treat cancer, headache, ulcers, and wounds [381].…”

Section: Herbs/spices Pharmacological Activity Lnp Type Referencesmentioning

confidence: 99%

Lipid Nanoparticles: An Effective Tool to Improve the Bioavailability of Nutraceuticals

Ashfaq,

Rasul,

Asghar

et al. 2023

IJMS

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Nano-range bioactive colloidal carrier systems are envisaged to overcome the challenges associated with treatments of numerous diseases. Lipid nanoparticles (LNPs), one of the extensively investigated drug delivery systems, not only improve pharmacokinetic parameters, transportation, and chemical stability of encapsulated compounds but also provide efficient targeting and reduce the risk of toxicity. Over the last decades, nature-derived polyphenols, vitamins, antioxidants, dietary supplements, and herbs have received more attention due to their remarkable biological and pharmacological health and medical benefits. However, their poor aqueous solubility, compromised stability, insufficient absorption, and accelerated elimination impede research in the nutraceutical sector. Owing to the possibilities offered by various LNPs, their ability to accommodate both hydrophilic and hydrophobic molecules and the availability of various preparation methods suitable for sensitive molecules, loading natural fragile molecules into LNPs offers a promising solution. The primary objective of this work is to explore the synergy between nature and nanotechnology, encompassing a wide range of research aimed at encapsulating natural therapeutic molecules within LNPs.

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Enhanced anticancer activity of encapsulated geraniol into biocompatible lipid nanoparticles against A549 human lung cancer cells

Cited by 14 publications

References 43 publications

Cytotoxic Screening and Enhanced Anticancer Activity of Lippia alba and Clinopodium nepeta Essential Oils-Loaded Biocompatible Lipid Nanoparticles against Lung and Colon Cancer Cells

Cytotoxic Screening and Enhanced Anticancer Activity of Lippia alba and Clinopodium nepeta Essential Oils-Loaded Biocompatible Lipid Nanoparticles against Lung and Colon Cancer Cells

Lipid Nanoparticles in Lung Cancer Therapy

Lipid Nanoparticles: An Effective Tool to Improve the Bioavailability of Nutraceuticals

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