Enhanced Antibacterial Ability and Bioactivity of Polyetherketoneketone Modified with LL-37

Jin, Yabing; Wang, Yijin; Chen, Yuhong; Han, Tianlei; Chen, Yiyi; Wang, Chen

doi:10.1021/acs.langmuir.1c03319

Cited by 9 publications

(8 citation statements)

References 52 publications

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“…The cells were collected and used between passages 3 and 5. The culture medium was refreshed every 2–3 days 29 …”

Section: Methodsmentioning

confidence: 99%

Enhanced osteogenic and antibacterial properties of polyetheretherketone by ultraviolet‐initiated grafting polymerization of a gelatin methacryloyl/epsilon‐poly‐L‐lysine/laponite hydrogel coating

Chen,

Han

et al. 2023

J Biomedical Materials Res

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Polyetheretherketone (PEEK) is a promising material for use in orthopedic implants, but its bio‐inert character and lack of antibacterial activity limit its applications in bone repair. In the present study, considering the advantages of PEEK in self‐initiated graft polymerization and of hydrogels in bone tissue engineering, we constructed a hydrogel coating (GPL) consisting of Gelatin methacryloyl (GelMA), methacrylamide‐modified ε‐poly‐l‐lysine (ε‐PLMA) and Laponite on PEEK through UV‐initiated crosslinking. The coating improved the hydrophilicity of PEEK, and the coating degraded slowly so that approximately 80% was retained after incubation in PBS for 8 weeks. In vitro studies revealed that as compared to culturing on PEEK, culturing on PEEK‐GPL led to enhanced viability and adhesion of cultured human umbilical cord Wharton's jelly‐derived mesenchymal stem cells (hWJ‐MSCs). Due to the synergistic effect of the micron‐scale three‐dimensional surface and Laponite, PEEK‐GPL exhibited a significantly improved induction of osteogenic differentiation of hWJ‐MSCs compared to PEEK, as demonstrated by increased alkaline phosphatase activity, matrix mineralization, and expression of osteogenesis‐related genes. Furthermore, PEEK‐GPL showed antibacterial activity upon contact with Staphylococcus aureus and Escherichia coli, and this activity would be maintained before complete degradation of the hydrogel because the ε‐PLMA was cross‐linked covalently into the coating. Thus, PEEK‐GPL achieved both osteogenesis and infection prevention in a single simple step, providing a feasible approach for the extensive use of PEEK in bone implants.

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“…The cells were collected and used between passages 3 and 5. The culture medium was refreshed every 2–3 days 29 …”

Section: Methodsmentioning

confidence: 99%

Enhanced osteogenic and antibacterial properties of polyetheretherketone by ultraviolet‐initiated grafting polymerization of a gelatin methacryloyl/epsilon‐poly‐L‐lysine/laponite hydrogel coating

Chen,

Han

et al. 2023

J Biomedical Materials Res

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“…The light absorption values were measured at 490 nm after the dimethyl sulfoxide was dissolved. [ 9b,43 ] Formazan accumulation was proportional to the number of viable cells. [ 9b,43b ] Strains TH and TH‐103Z were cultivated in LB medium or fermentation medium at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

Creating Polyploid Escherichia Coli and Its Application in Efficient L‐Threonine Production

Wang,

Chen,

Jin

et al. 2023

Advanced Science

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Prokaryotic genomes are generally organized in haploid. In synthetic biological research, efficient chassis cells must be constructed to produce bio‐based products. Here, the essential division of the ftsZ gene to create functional polyploid E. coli is regulated. The artificial polyploid E. coli containing 2–4 chromosomes is confirmed through PCR amplification, terminator localization, and flow cytometry. The polyploid E. coli exhibits a larger cell size, and its low pH tolerance and acetate resistance are stronger than those of haploid E. coli. Transcriptome analysis shows that the genes of the cell's main functional pathways are significantly upregulated in the polyploid E. coli. These advantages of the polyploid E. coli results in the highest reported L‐threonine yield (160.3 g L−1) in fed‐batch fermentation to date. In summary, an easy and convenient method for constructing polyploid E. coli and demonstrated its application in L‐threonine production is developed. This work provides a new approach for creating an excellent host strain for biochemical production and studying the evolution of prokaryotes and their chromosome functions.

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“…In addition, AMPs have highly selective toxicity to bacterial cell membranes and are not easy to damage mammalian cells ( Xi et al, 2016 ; Zhang et al, 2021 ). Recently, many studies have confirmed that AMPs could improve the antibacterial properties of biological materials ( Pranantyo et al, 2016 , 2018 , 2019 , 2021 ; Albada and Metzler-Nolte, 2017 ; Yang et al, 2017 ; Cao et al, 2018 ; Zhang et al, 2020 ; Wang H. et al, 2021 ; Yan et al, 2021 ; Jin et al, 2022 ). HHC36 (KRWWKWWRR) is a highly effective AMP containing nine amino acids predicted and designed by the artificial neural network.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial properties of antimicrobial peptide HHC36 modified polyetheretherketone

et al. 2023

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IntroductionPolyetheretherketone (PEEK) is considered to be a new type of orthopedic implant material due to its mechanical properties and biocompatibility. It is becoming a replacement for titanium (Ti) due to its near-human-cortical transmission and modulus of elasticity. However, its clinical application is limited because of its biological inertia and susceptibility to bacterial infection during implantation. To solve this problem, there is an urgent need to improve the antibacterial properties of PEEK implants.MethodsIn this work, we fixed antimicrobial peptide HHC36 on the 3D porous structure of sulfonated PEEK (SPEEK) by a simple solvent evaporation method (HSPEEK), and carried out characterization tests. We evaluated the antibacterial properties and cytocompatibility of the samples in vitro. In addition, we evaluated the anti-infection property and biocompatibility of the samples in vivo by establishing a rat subcutaneous infection model.ResultsThe characterization test results showed that HHC36 was successfully fixed on the surface of SPEEK and released slowly for 10 days. The results of antibacterial experiments in vitro showed that HSPEEK could reduce the survival rate of free bacteria, inhibit the growth of bacteria around the sample, and inhibit the formation of biofilm on the sample surface. The cytocompatibility test in vitro showed that the sample had no significant effect on the proliferation and viability of L929 cells and had no hemolytic activity on rabbit erythrocytes. In vivo experiments, HSPEEK can significantly reduce the bacterial survival rate on the sample surface and the inflammatory reaction in the soft tissue around the sample.DiscussionWe successfully loaded HHC36 onto the surface of SPEEK through a simple solvent evaporation method. The sample has excellent antibacterial properties and good cell compatibility, which can significantly reduce the bacterial survival rate and inflammatory reaction in vivo. The above results indicated that we successfully improved the antibacterial property of PEEK by a simple modification strategy, making it a promising material for anti-infection orthopedic implants.

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Enhanced Antibacterial Ability and Bioactivity of Polyetherketoneketone Modified with LL-37

Cited by 9 publications

References 52 publications

Enhanced osteogenic and antibacterial properties of polyetheretherketone by ultraviolet‐initiated grafting polymerization of a gelatin methacryloyl/epsilon‐poly‐L‐lysine/laponite hydrogel coating

Enhanced osteogenic and antibacterial properties of polyetheretherketone by ultraviolet‐initiated grafting polymerization of a gelatin methacryloyl/epsilon‐poly‐L‐lysine/laponite hydrogel coating

Creating Polyploid Escherichia Coli and Its Application in Efficient L‐Threonine Production

Antibacterial properties of antimicrobial peptide HHC36 modified polyetheretherketone

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