Enhanced angiogenic effects of RGD, GHK peptides and copper (II) compositions in synthetic cryogel ECM model

Zoughaib, Mohamed; Luong, Duong; Garifullin, Ruslan; Gatina, Dilara Z.; Fedosimova, Svetlana; Abdullin, Timur I.

doi:10.1016/j.msec.2020.111660

Cited by 20 publications

(22 citation statements)

References 54 publications

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“…The co-encapsulation of Co and Ca ions within gauze-alginate composite hydrogel resulted in enhanced local VEGF and TGF-β1 protein expression and accelerated wound healing in a mouse bacteria-infected wound model [ 18 ]. We have shown recently that macroporous hydrogels prepared by the cryogelation technique, namely, cryogels composed both of bio- and synthetic polymers, represent a promising type of scaffold for bulk bioactivation with TMs in a controllable and stable manner [ 19 , 20 ]. The advanced porous structure of cryogels ensures enhanced mammalian cell infiltration and activity of the incorporated metal dopant within the scaffold [ 19 , 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

“…We have shown recently that macroporous hydrogels prepared by the cryogelation technique, namely, cryogels composed both of bio- and synthetic polymers, represent a promising type of scaffold for bulk bioactivation with TMs in a controllable and stable manner [ 19 , 20 ]. The advanced porous structure of cryogels ensures enhanced mammalian cell infiltration and activity of the incorporated metal dopant within the scaffold [ 19 , 20 , 21 ]. The increased healing of an excisional skin defect treated with the Zn-doped gelatin cryogel [ 19 ] as well as enhanced in vitro angiogenic responses of poly(2-hydroxyethyl methacrylate) cryogels modified with Cu 2+ (via complexation with GHK peptide) [ 20 ] were demonstrated.…”

Section: Introductionmentioning

confidence: 99%

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Regenerative Activities of ROS-Modulating Trace Metals in Subcutaneously Implanted Biodegradable Cryogel

Yergeshov

Zoughaib

Ishkaeva

et al. 2022

Gels

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Divalent trace metals (TM), especially copper (Cu), cobalt (Co) and zinc (Zn), are recognized as essential microelements for tissue homeostasis and regeneration. To achieve a balance between therapeutic activity and safety of administered TMs, effective gel formulations of TMs with elucidated regenerative mechanisms are required. We studied in vitro and in vivo effects of biodegradable macroporous cryogels doped with Cu, Co or Zn in a controllable manner. The extracellular ROS generation by metal dopants was assessed and compared with the intracellular effect of soluble TMs. The stimulating ability of TMs in the cryogels for cell proliferation, differentiation and cytokine/growth factor biosynthesis was characterized using HSF and HUVEC primary human cells. Multiple responses of host tissues to the TM-doped cryogels upon subcutaneous implantation were characterized taking into account the rate of biodegradation, production of HIF-1α/matrix metalloproteinases and the appearance of immune cells. Cu and Zn dopants did not disturb the intact skin organization while inducing specific stimulating effects on different skin structures, including vasculature, whereas Co dopant caused a significant reorganization of skin layers, the appearance of multinucleated giant cells, along with intense angiogenesis in the dermis. The results specify and compare the prooxidant and regenerative potential of Cu, Co and Zn-doped biodegradable cryogels and are of particular interest for the development of advanced bioinductive hydrogel materials for controlling angiogenesis and soft tissue growth.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Regenerative Activities of ROS-Modulating Trace Metals in Subcutaneously Implanted Biodegradable Cryogel

Yergeshov

Zoughaib

Ishkaeva

et al. 2022

Gels

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“…The synergistic activity of RGD and GHK motifs was revealed in relation to the cell proliferation and behavior of 3T3 and PC-12 cells seeded on cryogel [ 163 ]. Furthermore, co-modification of cryogels with RGD and GHK, and then with Cu 2+ , enhanced regenerative angiogenic responses in vitro by a sharp increase in the cell proliferation, differentiation, and production of a number of cytokines related to angiogenesis and growth factors in HUVECs [ 164 ]. Although the proposed synthetic ECM-mimicking cryogel scaffold is non-biodegradable, it has proved to be an informative tool for studying multiple cell-responsive factors.…”

Section: Biodegradable Cryogelsmentioning

confidence: 99%

Design and Assessment of Biodegradable Macroporous Cryogels as Advanced Tissue Engineering and Drug Carrying Materials

Zoughaib

Yergeshov

2021

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Cryogels obtained by the cryotropic gelation process are macroporous hydrogels with a well-developed system of interconnected pores and shape memory. There have been significant recent advancements in our understanding of the cryotropic gelation process, and in the relationship between components, their structure and the application of the cryogels obtained. As cryogels are one of the most promising hydrogel-based biomaterials, and this field has been advancing rapidly, this review focuses on the design of biodegradable cryogels as advanced biomaterials for drug delivery and tissue engineering. The selection of a biodegradable polymer is key to the development of modern biomaterials that mimic the biological environment and the properties of artificial tissue, and are at the same time capable of being safely degraded/metabolized without any side effects. The range of biodegradable polymers utilized for cryogel formation is overviewed, including biopolymers, synthetic polymers, polymer blends, and composites. The paper discusses a cryotropic gelation method as a tool for synthesis of hydrogel materials with large, interconnected pores and mechanical, physical, chemical and biological properties, adapted for targeted biomedical applications. The effect of the composition, cross-linker, freezing conditions, and the nature of the polymer on the morphology, mechanical properties and biodegradation of cryogels is discussed. The biodegradation of cryogels and its dependence on their production and composition is overviewed. Selected representative biomedical applications demonstrate how cryogel-based materials have been used in drug delivery, tissue engineering, regenerative medicine, cancer research, and sensing.

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“…The most important features that distinguish cryogels from hydrogel equivalents are that they are highly porous materials with better mechanical strength and faster response times to external stimuli. It is possible to synthesize cryogel with other materials in composite formulations to further improve the innate properties [ 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 ]. For this purpose, PVA, a non-toxic biocompatible synthetic polymer with excellent physico-mechanical and viscoelastic properties [ 1 , 2 , 3 , 36 , 37 ], and a natural polyphenol TA and Cur possessing antioxidant, anti-inflammatory, anticancer, and antimicrobial activities [ 11 , 22 ] were used to design PVA:TA and PVA:TA:Cur cryogel composites.…”

Section: Introductionmentioning

confidence: 99%

Poly(vinyl alcohol)-tannic Acid Cryogel Matrix as Antioxidant and Antibacterial Material

et al. 2021

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The biocompatible, viscoelastic properties of poly(vinyl alcohol) (PVA) in combination with the antimicrobial and antioxidant natural polyphenolic, tannic acid (TA), and the natural flavonoid and antioxidant curcumin (Cur), were used in the preparation of PVA:TA and PVA:TA:Cur cryogel composites using cryotropic gelation to combine the individually beneficial properties. The effect of TA content on the antioxidant and antimicrobial activities of PVA:TA cryogel composites and the antioxidant activities of PVA:TA:Cur cryogel composites was determined using Trolox equivalent antioxidant capacity (TEAC) and total phenol content (TPC) assays, and were compared. The PVA:TA:Cur cryogel composite showed the highest antioxidant activity, with a TEAC value of 2.10 ± 0.24 and a TPC value of 293 ± 12.00. The antibacterial capacity of the PVA:TA and PVA:TA:Cur 1:1:0.1 cryogel composites was examined against two different species of bacteria, E. coli and S. aureus. It was found that the minimum inhibition concentration (MIC) value of the PVA:TA:Cur 1:1:0.1 cryogel composites varied between 5 and 10 mg/mL based on the type of microorganism, and the minimum bactericidal concentration (MBC) value was 20 mg/mL irrespective of the type of microorganism. Furthermore, the hemocompatibility of the PVA:TA cryogel composites was evaluated by examining their hemolytic and coagulation behaviors. PVA:TA 1:1 cryogels with a value of 95.7% revealed the highest blood clotting index value amongst all of the synthesized cryogels, signifying the potential for blood contacting applications. The release of TA and Cur from the cryogel composites was quantified at different pH conditions, i.e., 1.0, 7.4, and 9.0, and additionally in ethanol (EtOH) and an ethanol–water (EtOH:Wat) mixture. The solution released from the PVA:TA cryogels in PBS was tested for inhibition capability against α-glucosidase (E.C. 3.2.1.20). Concentration-dependent enzyme inhibition was observed, and 70 µL of 83 µg/mL PVA:TA (1:1) cryogel in PBS inhibited α-glucosidase enzyme solution of 0.03 unit/mL in 70 µL by 81.75 ± 0.96%.

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Enhanced angiogenic effects of RGD, GHK peptides and copper (II) compositions in synthetic cryogel ECM model

Cited by 20 publications

References 54 publications

Regenerative Activities of ROS-Modulating Trace Metals in Subcutaneously Implanted Biodegradable Cryogel

Regenerative Activities of ROS-Modulating Trace Metals in Subcutaneously Implanted Biodegradable Cryogel

Design and Assessment of Biodegradable Macroporous Cryogels as Advanced Tissue Engineering and Drug Carrying Materials

Poly(vinyl alcohol)-tannic Acid Cryogel Matrix as Antioxidant and Antibacterial Material

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