Enhanced and specific epoxidation activity of P450 BM3 mutants for the production of high value terpene derivatives

Correddu, Danilo; Aly, Sabrina Helmy; Nardo, Giovanna Di; Catucci, Gianluca; Prandi, Cristina; Blangetti, Marco; Bellomo, Chiara; Bonometti, Elisabetta; Viscardi, G.; Gilardi, Gianfranco

doi:10.1039/d2ra06029a

Cited by 7 publications

(5 citation statements)

References 38 publications

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“…The more reported linalool derivatives in tea are linalool oxides, and 8-hydroxylinalool is rarely reported, which might be related to its trace amounts in many tea plants. Linalool oxide and 8-hydroxylinalool are derived from two pathways with linalool as the precursor, and linalool forms linalool oxide by intermediate 6,7-epoxylinalool ( Correddu et al, 2022 , Meesters et al, 2007 , Raguso and Pichersky, 1999 ), but it is directly hydroxylated at C8 to form 8-hydroxylinalool. In many tea plants, the enzymes involved in the synthesis of linalool oxide may have a better affinity to the substrate linalool, thus biasing the metabolic flux toward linalool oxide.…”

Section: Resultsmentioning

confidence: 99%

Formation of 8-hydroxylinalool in tea plant Camellia sinensis var. Assamica ‘Hainan dayezhong’

Zhou

et al. 2023

Food Chemistry: Molecular Sciences

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Section: Resultsmentioning

confidence: 99%

Formation of 8-hydroxylinalool in tea plant Camellia sinensis var. Assamica ‘Hainan dayezhong’

Zhou

et al. 2023

Food Chemistry: Molecular Sciences

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“…[61] CÀ H Activation Cytochrome P450 monooxygenases are ubiquitous enzymes primarily used for selective oxidation reactions, e.g. hydroxylation [62] and epoxidation [63] including the late-stage functionalisation of many natural products. [64,65] The majority of P450 monooxygenases require a separate ferredoxin reductase for electron shuttling, promoting the search for self-sufficient P450 enzymes with a tethered FAD/FMN reductase domain.…”

Section: C=x Bond Reductionmentioning

confidence: 99%

The Impact of Metagenomics on Biocatalysis

Hogg,

Schnepel,

Finnigan

et al. 2024

Angewandte Chemie

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In the ever‐growing demand for sustainable ways to produce high‐value small molecules, biocatalysis has come to the forefront of greener routes to these chemicals. As such, the need to constantly find and optimise suitable biocatalysts for specific transformations has never been greater. Metagenome mining has been shown to rapidly expand the toolkit of promiscuous enzymes needed for new transformations, without requiring protein engineering steps. If protein engineering is needed, the metagenomic candidate can often provide a better starting point for engineering than a previously discovered enzyme on the open database or from literature, for instance. In this review, we highlight where metagenomics has made substantial impact on the area of biocatalysis in recent years. We review the discovery of enzymes in previously unexplored or ‘hidden’ sequence space, leading to the characterisation of enzymes with enhanced properties that originate from natural selection pressures in native environments.

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“…Indeed, by the addition of H 2 O 2 as source of oxygen and electrons for the P450 catalytic cycle it is possible to avoid the use of the expensive cofactor(s) NAD(P)H and redox partner(s) reactions, that are often a source of uncoupling. [ 39 ] CYP116B5 heme domain (CYP116B5‐hd) has been isolated by protein engineering and characterized for its ability to convert drugs using H 2 O 2 as catalysis driving agent. [ 21 ] Nevertheless, the oxidative stress resulting from the use of H 2 O 2 is disadvantageous for protein stability and toxic for cells.…”

Section: Introductionmentioning

confidence: 99%

CYP116B5‐SOX: An artificial peroxygenase for drug metabolites production and bioremediation

Giuriato,

Catucci,

Correddu

et al. 2024

Biotechnology Journal

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CYP116B5 is a class VII P450 in which the heme domain is linked to a FMN and 2Fe2S‐binding reductase. Our laboratory has proved that the CYP116B5 heme domain (CYP116B5‐hd) is capable of catalyzing the oxidation of substrates using H2O2. Recently, the Molecular Lego approach was applied to join the heme domain of CYP116B5 to sarcosine oxidase (SOX), which provides H2O2 in‐situ by the sarcosine oxidation. In this work, the chimeric self‐sufficient fusion enzyme CYP116B5‐SOX was heterologously expressed, purified, and characterized for its functionality by absorbance and fluorescence spectroscopy. Differential scanning calorimetry (DSC) experiments revealed a TM of 48.4 ± 0.04 and 58.3 ± 0.02°C and a enthalpy value of 175,500 ± 1850 and 120,500 ± 1350 cal mol−1 for the CYP116B5 and SOX domains respectively. The fusion enzyme showed an outstanding chemical stability in presence of up to 200 mM sarcosine or 5 mM H2O2 (4.4 ± 0.8 and 11.0 ± 2.6% heme leakage respectively). Thanks to the in‐situ H2O2 generation, an improved kcat/KM for the p‐nitrophenol conversion was observed (kcat of 20.1 ± 0.6 min−1 and KM of 0.23 ± 0.03 mM), corresponding to 4 times the kcat/KM of the CYP116B5‐hd. The aim of this work is the development of an engineered biocatalyst to be exploited in bioremediation. In order to tackle this challenge, an E. coli strain expressing CYP116B5‐SOX was employed to exploit this biocatalyst for the oxidation of the wastewater contaminating‐drug tamoxifen. Data show a 12‐fold increase in tamoxifen N‐oxide production—herein detected for the first time as CYP116B5 metabolite—compared to the direct H2O2 supply, equal to the 25% of the total drug conversion.

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Enhanced and specific epoxidation activity of P450 BM3 mutants for the production of high value terpene derivatives

Abstract: Laboratory evolved P450 BM3 A2 has an increased activity on the oxidation of high value terpenes. The oxidation of linalool into linalool oxide by P450 BM3 is shown for the first time.

Cited by 7 publications

References 38 publications

Formation of 8-hydroxylinalool in tea plant Camellia sinensis var. Assamica ‘Hainan dayezhong’

Formation of 8-hydroxylinalool in tea plant Camellia sinensis var. Assamica ‘Hainan dayezhong’

The Impact of Metagenomics on Biocatalysis

CYP116B5‐SOX: An artificial peroxygenase for drug metabolites production and bioremediation

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