Enhanced Agarose and Xylan Degradation for Production of Polyhydroxyalkanoates by Co-Culture of Marine Bacterium, Saccharophagus degradans and Its Contaminant, Bacillus cereus

Sawant, Shailesh S.; Salunke, Bipinchandra K.; Taylor, Larry E.; Kim, Beom Soo

doi:10.3390/app7030225

Cited by 25 publications

(7 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Co-cultures work synergistically to convert feedstock into PHA as was carried out in case of synthetic plastic pyrolysis oil by three strains of genus Pseudomonas putida into PHA [9]. This indicates that in co-culture environment, bacteria cooperate with each other, enhance feedstock consumption, and PHA production [10,11].…”

Section: Introductionmentioning

confidence: 86%

Polyhydroxyalkanoates (PHA) production in bacterial co‐culture using glucose and volatile fatty acids as carbon source

Munir

Jamil

2018

J Basic Microbiol

View full text Add to dashboard Cite

Mixed bacterial cultures are increasingly being used in the production of polyhydroxyalkanoates (PHAs), as they have the potential to be more cost effective than axenic pure cultures. The purpose of this study was to use pure cultures in combination to identify their potential of PHA production. In this work we used volatile fatty acids (VFAs) and glucose as carbon source to check the ability of selected strains ST2 (Pseudomonas sp.) and CS8 (Bacillus sp.) as co-culture. The production of PHA in pure co-cultures of bacteria was therefore investigated in order to understand the effect of combining cultures on PHA production parameters and material properties. Bacteria could use the feed in better way when mixed as compared to individual strain. In undertaking this analysis, model volatile fatty acids (i.e., acetic and propionic acids) were used alone and in combination with glucose as feedstock. The production by Pseudomonas was 34% while 24% by Bacillus. However when combined and mixed feed (glucose + propionic acid) was used, 35% PHA produced. Overall, it was found that the ability of the pure cultures to produce PHA was low but when selected cultures were mixed, their ability to produce PHA was enhanced. Copolymers were obtained instead of homopolymers with improved properties. This suggests that industrial wastewater rich in volatile fatty acids and carbohydrates can be a good carbon source for PHA production with variable properties.

show abstract

Section: Introductionmentioning

confidence: 86%

Polyhydroxyalkanoates (PHA) production in bacterial co‐culture using glucose and volatile fatty acids as carbon source

Munir

Jamil

2018

J Basic Microbiol

View full text Add to dashboard Cite

show abstract

“…Recently, another approach towards PHA biosynthesis from S. degradans was proposed. During their experiments, Sawant et al [ 24 ] observed that Bacillus cereus (KF801505) was growing together with S. degradans 2-40 as a contaminant and had the ability of producing high amounts of PHAs [ 25 ]. In addition, the viability and agar degradation potential of S. degradans increased with the presence of B. cereus .…”

Section: Pha Production By Pure Bacterial Culturesmentioning

confidence: 99%

Recent Advances and Challenges towards Sustainable Polyhydroxyalkanoate (PHA) Production

et al. 2017

View full text Add to dashboard Cite

Sustainable biofuels, biomaterials, and fine chemicals production is a critical matter that research teams around the globe are focusing on nowadays. Polyhydroxyalkanoates represent one of the biomaterials of the future due to their physicochemical properties, biodegradability, and biocompatibility. Designing efficient and economic bioprocesses, combined with the respective social and environmental benefits, has brought together scientists from different backgrounds highlighting the multidisciplinary character of such a venture. In the current review, challenges and opportunities regarding polyhydroxyalkanoate production are presented and discussed, covering key steps of their overall production process by applying pure and mixed culture biotechnology, from raw bioprocess development to downstream processing.

show abstract

“…Therefore, there is a concern that they may damage the marine ecosystem in a manner similar to the damage caused by commodity plastics. In addition to the aliphatic polyesters PHA and PCL, several polymers, such as cellulose [40][41][42], agarose [43,44], proteins [45], and poly(vinyl alcohol) (PVA) [46], have been suggested to exhibit relatively high biodegradability in marine environments.…”

Section: Enzymatic and Environmental Degradability Of Biodegradable Plasticsmentioning

confidence: 99%

Biodegradability of poly(3-hydroxyalkanoate) and poly(ε-caprolactone) via biological carbon cycles in marine environments

Suzuki

Tachibana

Kasuya

2020

Polym J

160

View full text Add to dashboard Cite

Approximately 4.8–12.7 million tons of plastic waste has been estimated to be discharged into marine environments annually by wind and river currents. The Ellen MacArthur Foundation warns that the total weight of plastic waste in the oceans will exceed the total weight of fish in 2050 if the environmental runoff of plastic continues at the current rate. Hence, biodegradable plastics are attracting attention as a solution to the problems caused by plastic waste. Among biodegradable plastics, polyhydroxyalkanoates (PHAs) and poly(ε-caprolactone) (PCL) are particularly noteworthy because of their excellent marine biodegradability. In this review, the biosynthesis of PHA and cutin, a natural analog of PCL, and the biodegradation of PHA and PCL in carbon cycles in marine ecosystems are discussed. PHA is biosynthesized and biodegraded by various marine microbes in a wide range of marine environments, including coastal, shallow-water, and deep-sea environments. Marine cutin is biosynthesized by marine plants or obtained from terrestrial environments, and PCL and cutin are biodegraded by cutin hydrolytic enzyme-producing microbes in broad marine environments. Thus, biological carbon cycles for PHA and PCL exist in the marine environment, which would allow materials made of PHA and PCL to be quickly mineralized in marine environments.

show abstract

Enhanced Agarose and Xylan Degradation for Production of Polyhydroxyalkanoates by Co-Culture of Marine Bacterium, Saccharophagus degradans and Its Contaminant, Bacillus cereus

Cited by 25 publications

References 63 publications

Polyhydroxyalkanoates (PHA) production in bacterial co‐culture using glucose and volatile fatty acids as carbon source

Polyhydroxyalkanoates (PHA) production in bacterial co‐culture using glucose and volatile fatty acids as carbon source

Recent Advances and Challenges towards Sustainable Polyhydroxyalkanoate (PHA) Production

Biodegradability of poly(3-hydroxyalkanoate) and poly(ε-caprolactone) via biological carbon cycles in marine environments

Contact Info

Product

Resources

About