Enhanced aerobic respiration improves in vitro coconut embryo germination and culture

Aké, A. E. Pech y; Souza, R.; Maust, B.E.; Santamaría, Jorge M.; Oropeza, Carlos

doi:10.1079/ivp2003480

Cited by 16 publications

(7 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some of the peculiarities of the embryos of A. aculeata are related to the emission of large numbers of vascular traces by the embryo axis, and to the smaller numbers of ramifications of the procambial strands in the direction of the haustorium, as compared to P. dactylifera (Demason and Thomson 1981), W. filifera (Demason 1988a), and Euterpe edulis (Panza et al 2004), as well as the bulgeshape of the haustorium, and the occurrence of prominent invaginations in the protoderm (Moura et al 2010). The present report of the occurrence of stomata in A. aculeata embryos is the first for Arecaceae, and these structures may reflect high oxygen demands in the initial phases of its germination, as pointed out by Bewley and Black (1994) in a general sense, and by Pech y Aké et al (2004) for the embryos of Cocos nucifera.…”

Section: Discussionsupporting

confidence: 71%

“…Structural evaluations of embryos and seedlings cultivated under in vitro conditions can furnish useful information that can improve culturing techniques, define morphological parameters indicative of germination, and standardize the terminology used in describing the seedlings. A number of published studies with palm embryos have not clearly identified the morphological criteria utilized to recognize germination (Ledo et al 2001;Pech y Aké et al 2004;Lédo et al 2007;Pereira et al 2006;Tzec-Sima et al 2006;Pech y Aké et al 2007). Melo et al (2001) considered seedlings of Syagrus oleracea to have germinated even without visible root system development, while Bandeira (2008) considered seedlings as the individuals of A. aculeata with only the root system emerged, or only the aerial portion, or both.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Structural evaluations of zygotic embryos and seedlings of the macaw palm (Acrocomia aculeata, Arecaceae) during in vitro germination

Ribeiro

Oliveira

Garcia

2011

Trees

View full text Add to dashboard Cite

Acrocomia aculeata is an oil producing tropical palm tree with exceptional potential for producing biofuel.As the propagation of this species is often difficult because of its pronounced seed dormancy, the present work examined the morphology and the anatomy of zygotic embryos and seedlings during in vitro germination. Embryos were put in MS media supplemented with organic compounds and cultivated in the dark at 30°C for 20 days. The dry weights, lengths, and diameters of the cotyledonary petioles, haustoria, roots, ligules, and leaf sheaths of embryos obtained from mature seeds and seedlings removed from culture were measured every 2 days; anatomical and histochemical evaluations were performed on embryos and seedlings removed from culture after 2, 5, 8, 10, 12, and 15 days. Elongation of the embryo axis was observed to initiate after 2 days. Elongation of the cotyledonary petiole was observed starting on the fifth day; this is a morphological indication of germination that is associated with the formation of starch and raphides as well as the differentiation of tracheary elements. The growth of the cotyledon is due to increases in cell volumes as well as the development of a meristematic band peripheral to the haustorium. In spite of the fact that the radicle is less differentiated than the plumule, radicular development is precocious and the root emerges first, indicating the absence of morphological dormancy. Atrophy of the haustorium and the accumulation of phenolic compounds in subepidermal cell layers occur due to culturing conditions.

show abstract

Section: Discussionsupporting

confidence: 71%

Section: Introductionmentioning

confidence: 99%

Structural evaluations of zygotic embryos and seedlings of the macaw palm (Acrocomia aculeata, Arecaceae) during in vitro germination

Ribeiro

Oliveira

Garcia

2011

Trees

View full text Add to dashboard Cite

show abstract

“…The seeds of the palm tree E . guineensis overcame dormancy when exposed to saturated atmospheric oxygen conditions (Hussey ), and treatments that increased oxygen availability and aerobic respiration were observed to favor the in vitro germination of Cocos nucifera L. embryos (Pech y Aké et al ). The presence of stomata in the proximal region of the cotyledonary petiole in macaw palm was considered an indication that gas exchange was especially important for germination (Ribeiro et al ).…”

Section: Discussionmentioning

confidence: 99%

“…The presence of stomata in the proximal region of the cotyledonary petiole in macaw palm was considered an indication that gas exchange was especially important for germination (Ribeiro et al ). Success in overcoming palm seed dormancy by removing the operculum (Hussey , Carpenter et al , Neves et al , Oliveira et al ) or by in vitro cultivation of isolated embryos (Pech y Aké et al , , Ribeiro et al , ) has been attributed to the removal of mechanical restrictions on the embryo (Neves et al , Magalhães et al , Oliveira et al ). We propose here that germination is stimulated by alterations in GA/ABA ratios, and possibly by metabolic alterations caused by greater oxygen availability.…”

Section: Discussionmentioning

confidence: 99%

Tissue‐specific hormonal profiling during dormancy release in macaw palm seeds

Ribeiro

Garcia

Müller

et al. 2014

Physiologia Plantarum

View full text Add to dashboard Cite

Little is known about the control exerted by hormones in specific tissues during germination and post-germinative development in monocot seeds, whose embryos have complex structures and can remain dormant for long periods of time. Here the tissue-specific hormonal profile of macaw palm (Acrocomia aculeata) seeds overcoming dormancy and seedling during initial development was examined. Endogenous hormonal concentrations were determined in the cotyledonary petiole, haustorium, operculum, endosperm adjacent to the embryo and peripheral endosperm of dry dormant seeds, imbibed seeds trapped in phase I of germination, and germinating (phase 2 and phase 3) seeds 2, 5, 10 and 15 days after sowing. Evaluations were performed on seeds treated for overcoming dormancy by removal of the operculum and by immersion in a gibberellic acid (GA3 ) solution. Removal of the operculum effectively helped in overcoming dormancy, which was associated with the synthesis of active gibberellins (GAs) and cytokinins (CKs), as well as reductions of abscisic acid (ABA) in the cotyledonary petiole. In imbibed seeds trapped in phase I of germination, exogenous GA3 caused an increase in active GAs in the cotyledonary petiole and operculum and reduction in ABA in the operculum. Initial seedling development was associated with increases in the CK/auxin ratio in the haustorium and GA levels in the endosperm which is possibly related to the mobilization of metabolic reserves. Increases in salicylic acid (SA) and jasmonic acid (JA) concentrations were associated with the development of the vegetative axis. Hormones play a crucial tissue-specific role in the control of dormancy, germination and initial development of seedlings in macaw palm, including a central role not only for GAs and ABA, but also for CKs and other hormones.

show abstract

“…Even though SE can be achieved in many species, it has been much more difficult to achieve in others, and this includes the coconut. The first attempts at coconut SE were undertaken over 30 years ago at Wye College, UK (Eeuwens and Blake 1977), and then by ORSTOM, France (Pannetier and Buffard-Morel 1982). These and other early studies used a number of plant somatic tissues as initial explants (i.e., young leaves, stem slices from young seedlings, sections from rachillae of young inflorescences) to form embryogenic calli (Branton and Blake 1983;Gupta et al 1984).…”

Section: Clonal Propagation Via Somatic Embryogenesis Somatic Embryogmentioning

confidence: 99%

Tissue culture and associated biotechnological interventions for the improvement of coconut (Cocos nucifera L.): a review

Nguyen

Bandupriya

López‐Villalobos

et al. 2015

Planta

View full text Add to dashboard Cite

The present review discusses not only advances in coconut tissue culture and associated biotechnological interventions but also future research directions toward the resilience of this important palm crop. Coconut (Cocos nucifera L.) is commonly known as the 'tree of life'. Every component of the palm can be used to produce items of value and many can be converted into industrial products. Coconut cultivation faces a number of acute problems that reduce its productivity and competitiveness. These problems include various biotic and abiotic challenges as well as an unstable market for its traditional oil-based products. Around 10 million small-holder farmers cultivate coconut palms worldwide on c. 12 million hectares of land, and many more people own a few coconut palms that contribute to their livelihoods. Inefficiency in the production of seedlings for replanting remains an issue; however, tissue culture and other biotechnological interventions are expected to provide pragmatic solutions. Over the past 60 years, much research has been directed towards developing and improving protocols for (i) embryo culture; (ii) clonal propagation via somatic embryogenesis; (iii) homozygote production via anther culture; (iv) germplasm conservation via cryopreservation; and (v) genetic transformation. Recently other advances have revealed possible new ways to improve these protocols. Although effective embryo culture and cryopreservation are now possible, the limited frequency of conversion of somatic embryos to ex vitro seedlings still prevents the large-scale clonal propagation of coconut. This review illustrates how our knowledge of tissue culture and associated biotechnological interventions in coconut has so far developed. Further improvement of protocols and their application to a wider range of germplasm will continue to open up new horizons for the collection, conservation, breeding and productivity of coconut.

show abstract

Enhanced aerobic respiration improves in vitro coconut embryo germination and culture

Cited by 16 publications

References 4 publications

Structural evaluations of zygotic embryos and seedlings of the macaw palm (Acrocomia aculeata, Arecaceae) during in vitro germination

Structural evaluations of zygotic embryos and seedlings of the macaw palm (Acrocomia aculeata, Arecaceae) during in vitro germination

Tissue‐specific hormonal profiling during dormancy release in macaw palm seeds

Tissue culture and associated biotechnological interventions for the improvement of coconut (Cocos nucifera L.): a review

Contact Info

Product

Resources

About