Enhanced adsorption of Congo red using chitin suspension after sonoenzymolysis

Hou, Furong; Wang, Danli; Ma, Xiaobin; Fan, Lihua; Ding, Tian; Ye, Xingqian; Liu, Donghong

doi:10.1016/j.ultsonch.2020.105327

Cited by 40 publications

(13 citation statements)

References 45 publications

(38 reference statements)

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“…The pore size distribution and the surface area of the biosorbent was obtained using the Barrett-Joyner-Halenda (BJH) and Brunaure-Emmet-Teller (BET) method, respectively [30]. The Pore surface characteristics has been compared to previous studies [31][32][33][34]. The surface properties of the biosorbents are shown in Table 1.…”

Section: Characterization Of Adsorbentsmentioning

confidence: 99%

Sustainable Durio zibethinus-Derived Biosorbents for Congo Red Removal from Aqueous Solution: Statistical Optimization, Isotherms and Mechanism Studies

et al. 2021

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This investigation reports on the biosorption mechanism of Congo Red dyes (CR) in aqueous solution using acid-treated durian peels, prepared for this study. The biosorbent nature was characterized using the Scanning Electron Microscopy (SEM), Fourier Transform infrared spectroscopy (FT-IR) and Brunaure-Emmet-Teller (BET). The effect of process parameters within operational range of pH (2–9), contact time (10–200 min), initial concentration (25–400 mg g−1) and temperature (25–65 °C) for the optimum removal of CR dyes was investigated using central composite design (CCD) under response surface methodology (RSM), and revealed that the optimum condition of biosorption was achieved around a pH of 5.5, contact time of 105 min at initial concentration of 212.5 mg L−1 within 45 °C temperature, which corresponds to 95.2% percent removal of CR. The experimental data fitted better to the second order polynomial model, with a correlation coefficient R2 value of 0.9917 and the Langmuir isotherm model with biosorption capacity of 107.52 mg g−1. Gibbs free energy indicated that the adsorption of CR dyes was spontaneous. The mechanism of the adsorption of CR dyes revealed that the biosorption of CR dyes investigated under different operational conditions show that under acidic pH, the adsorption efficiency of the acid treated durian peels is enhanced for the adsorption of CR dye molecules.

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Section: Characterization Of Adsorbentsmentioning

confidence: 99%

Sustainable Durio zibethinus-Derived Biosorbents for Congo Red Removal from Aqueous Solution: Statistical Optimization, Isotherms and Mechanism Studies

et al. 2021

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“…Three mutants completely lost all catalytic activity confirmed by both microplate-reader and Congo red dying methods (Figure 4b,c). Congo red-chitin complex is red color as Congo red and chitin combine [51], while the red color disappeared if the chitin in the complex was degraded. It was transparent around the wild-type MxChi treated media (Figure 4c), while the media treated by mutants of MxChi had no change in red color, implying that the mutants were inactive, which is consistent with that of the microplate-reader testing, suggesting these three amino acids are parts of the active sites.…”

Section: Mutational Analysismentioning

confidence: 99%

Heterologous Expression of a Thermostable Chitinase from Myxococcus xanthus and Its Application for High Yield Production of Glucosamine from Shrimp Shell

Lyu

Zheng

Qiu

et al. 2021

Foods

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Glucosamine (GlcN) is a widely used food supplement. Hence, enormous attention has been concerned with enzymatic production of GlcN owing to its advantage over a chemical approach. In this study, a previously unstudied chitinase gene (MxChi) in the genome of Myxococcus xanthus was cloned, expressed in recombinant soluble form and purified to homogeneity. TLC-, UPLC-, and microplate-reader- based activity tests confirmed MxChi hydrolyzes colloidal chitin to chitobiose as sole product. The optimal catalytic pH and temperature of MxChi was identified as 7.0 and 55 °C, respectively. MxChi exhibited 80% activity after 72 h incubation at 37 °C. The site-directed mutagenesis revealed that the amino acids D323A, D325A, and E327A of MxChi were in the DXDXE catalytic motif of GH18. When coupled with β-N-acetylhexosaminidase (SnHex) and deacetylase (CmCBDA), the enzyme allowed one-pot extraction of GlcN from colloidal chitin and shrimp shell. The optimal condition was 37 °C, pH 8.0, and 1/3/16.5 (MxChi/SnHex/CmCBDA), conducted by orthogonal design for the enzymatic cascades. Under this condition, the yield of GlcN was 26.33 mg from 400 mg shrimp shell. Facile recombinant in E. coli, robust thermostability and pure product herein makes newly discovered chitinase a valuable candidate for the green recycling of chitin rich waste.

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“…8 Polymers are macromolecules, which are widely distributed in nature and perform several functions in everyday life. Polymers can be divided into two main categories, e.g., (I) bio-based polymers such as chitin, 9 CS, 10 silk fibroin, 11 keratin, 12 starch, 13 etc., and (II) synthetic polymers such as polyethylene, 14 polyethylene terephthalate, 15 polystyrene, 16 and GMA, 17 etc. Bio-based polymers have a non-toxic, biodegradable, economical, and environmentally friendly nature.…”

Section: Introductionmentioning

confidence: 99%

“…Polymers can be divided into two main categories, e.g. , (I) bio-based polymers such as chitin, 9 CS, 10 silk fibroin, 11 keratin, 12 starch, 13 etc. , and (II) synthetic polymers such as polyethylene, 14 polyethylene terephthalate, 15 polystyrene, 16 and GMA, 17 etc.…”

Section: Introductionmentioning

confidence: 99%

Chitosan- or glycidyl methacrylate-based adsorbents for the removal of dyes from aqueous solutions: a review

2022

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Enhanced adsorption of Congo red using chitin suspension after sonoenzymolysis

Cited by 40 publications

References 45 publications

Sustainable Durio zibethinus-Derived Biosorbents for Congo Red Removal from Aqueous Solution: Statistical Optimization, Isotherms and Mechanism Studies

Sustainable Durio zibethinus-Derived Biosorbents for Congo Red Removal from Aqueous Solution: Statistical Optimization, Isotherms and Mechanism Studies

Heterologous Expression of a Thermostable Chitinase from Myxococcus xanthus and Its Application for High Yield Production of Glucosamine from Shrimp Shell

Chitosan- or glycidyl methacrylate-based adsorbents for the removal of dyes from aqueous solutions: a review

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