Enhanced Activity of the Nucleopolyhedrovirus of the Fall Armyworm (Lepidoptera: Noctuidae) on Bt-Transgenic and Nontransgenic Sweet Corn with a Fluorescent Brightener and a Feeding Stimulant

Farrar, Robert R.; Shapiro, Martin; Shepard, B. Merle

doi:10.1603/0046-225x-34.4.825

Cited by 9 publications

(8 citation statements)

References 30 publications

(33 reference statements)

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“…The lesser effect on damage is probably due to high pest pressure; though reduced in numbers, there were still enough larvae to cause plants to be classified as damaged. Previous studies of the fall armyworm showed that the effect of SfMNPV was greater on nontransgenic corn than on Bt corn ( Farrar et al 2004 , 2005 ). In the present study, reductions in densities of fall armyworms were seen on both genotypes of corn, and there were no significant interactions of spray treatment by corn genotype in most analyses of these variables.…”

Section: Discussionmentioning

confidence: 96%

“…The SfMNPV used in this study was originally isolated from fall armyworm larvae collected from the field in Georgia (USA) by J. J. Hamm (USDA-ARS, Tifton, GA, USA). For the tests reported herein, it was produced in our laboratory in fall armyworm larvae as described by Farrar et al ( 2004 , 2005 ) and prepared as an aqueous suspension containing 2 × 10 9 occlusion bodies/ml. HzSNPV (Gemstar® LC, 2 × 10 9 occlusion bodies/ml), neem extract (Neemix® 4.5, liquid, 4.5% azadirachtin), sprayable B. thuringiensis (CryMax® WDG, 40% Bt solids, spores, and toxins), and the nematode Steinernema riobrave (Cabanillas, Poinar and Raulston) (Biovector 355®, 3.83 × 105 infective juveniles/g) were obtained from Certis USA ( www.certisusa.com ).…”

Section: Methodsmentioning

confidence: 99%

“…In laboratory tests, fall armyworm larvae consumed less foliage of sweet corn expressing a Cry1Ab toxin than they did of nontransgenic sweet corn foliage. When larvae were fed foliage treated with SfMNPV, reduced rates of consumption led to lower rates of virus-induced mortality on transgenic sweet corn relative to those on nontransgenic sweet corn ( Farrar et al 2004 , 2005 ).…”

Section: Introductionmentioning

confidence: 99%

“…The present study was undertaken to evaluate a wide range of biologically-based tactics as supplemental controls for the fall armyworm and the corn earworm on Bt transgenic sweet corn, and to extend previous laboratory work on NPVs ( Farrar et al 2004 , 2005 ) to the field. Therefore, while the primary focus was on NPVs, a number of other, commercially available, materials were also included.…”

Section: Introductionmentioning

confidence: 99%

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Supplemental Control of Lepidopterous Pests on Bt Transgenic Sweet Corn with Biologically-Based Spray Treatments

Farrar

Shepard²,

Shapiro³

et al. 2009

Journal of Insect Science

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Biologically-based spray treatments, including nucleopolyhedroviruses, neem, and spinosad, were evaluated as supplemental controls for the fall armyworm, Spodoptera frugiperda (J. E. Smith), and corn earworm, Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae), on transgenic sweet corn, Zea mays (L.) (Poales: Poaceae), expressing a Cry1Ab toxin from Bacillus thuringiensis Berliner (Bacillales: Bacillaceae) (Bt). Overall, transgenic corn supported lower densities of both pests than did nontransgenic corn. Control of the fall armyworm was improved in both whorl-stage and tassel-stage corn by the use of either a nucleopolyhedrovirus or neem, but the greatest improvement was seen with spinosad. Only spinosad consistently reduced damage to ears, which was caused by both pest species. In general, efficacy of the spray materials did not differ greatly between transgenic and nontransgenic corn.

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Section: Discussionmentioning

confidence: 96%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Supplemental Control of Lepidopterous Pests on Bt Transgenic Sweet Corn with Biologically-Based Spray Treatments

Farrar

Shepard²,

Shapiro³

et al. 2009

Journal of Insect Science

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“…The S. frugiperda NPVs isolated to date appear to be variants of the same virus species, S. frugiperda multiple nucleopolyhedrovirus (SfMNPV). Isolates of SfMNPV have been evaluated as biopesticides to control infestations of S. frugiperda on maize [13][14][15][16][17][18][19][20], and studies on NPV ecology have used SfMNPV as a model virus [21].…”

Section: Introductionmentioning

confidence: 99%

Genetic and biological variation among nucleopolyhedrovirus isolates from the fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae)

et al. 2010

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A PCR-based method was used to identify and distinguish among 40 uncharacterized nucleopolyhedrovirus (NPV) isolates from larvae of the moth Spodoptera frugiperda that were part of an insect virus collection. Phylogenetic analysis was carried out with sequences amplified from two strongly conserved loci (polh and lef-8) from the 40 isolates in the collection and from eight previously studied S. frugiperda NPV (SfMNPV) isolates. To further distinguish these isolates, analysis was also carried out with sequences from two less-conserved loci, hr4 and hr5. Phylogenetic inference from the sequence data could distinguish among several of the individual isolates and between different groups of isolates from Georgia (USA) and Colombia, South America. A stronger degree of bootstrap support for the phylogenetic trees was obtained with the hr4 and hr5 homologous repeat sequences. Sequencing and phylogenetic analysis detected a relatively high degree of larva-to-larva sequence divergence occurring among isolates of SfMNPV collected from the same field in Missouri, USA. Restriction endonuclease analysis of viral DNA from larvae infected with five isolates from Georgia, Missouri, Louisiana, Florida (USA), and Colombia allowed for further comparison with other previously reported isolates of SfMNPV. Bioassays with these five geographically distinct isolates detected minor differences in virulence. This study highlights the use of PCR to rapidly distinguish and characterize large numbers of historical baculovirus isolates from the same host using minimal quantities of material, and the use of sequences from homologous repeat regions to distinguish closely related isolates of the same NPV species.

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Can a chitin‐synthesis‐inhibiting turfgrass fungicide enhance black cutworm susceptibility to a baculovirus?

Bixby-Brosi

Potter

2011

Pest Management Science

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Enhanced Activity of the Nucleopolyhedrovirus of the Fall Armyworm (Lepidoptera: Noctuidae) on Bt-Transgenic and Nontransgenic Sweet Corn with a Fluorescent Brightener and a Feeding Stimulant

Cited by 9 publications

References 30 publications

Supplemental Control of Lepidopterous Pests on Bt Transgenic Sweet Corn with Biologically-Based Spray Treatments

Supplemental Control of Lepidopterous Pests on Bt Transgenic Sweet Corn with Biologically-Based Spray Treatments

Genetic and biological variation among nucleopolyhedrovirus isolates from the fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae)

Can a chitin‐synthesis‐inhibiting turfgrass fungicide enhance black cutworm susceptibility to a baculovirus?

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