Enhanced acetoin production by Serratia marcescens H32 with expression of a water-forming NADH oxidase

Sun, Jianan; Zhang, Liaoyuan; Ben, Rao; Shen, Yang; Wei, Dongzhi

doi:10.1016/j.biortech.2012.05.108

Cited by 96 publications

(59 citation statements)

References 24 publications

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“…However, the yields were both low (0.14 and 3.52 g L −1 of TMP, respectively) due to weak accumulation of the precursor acetoin (1.6 and 12.7 g L −1 of acetoin, respectively). The current record of acetoin fermentation is 75.2 g L −1 using S. marcescens H32-nox [30]. According to the top 10 records of acetoin fermentation [31], some Bacillus strains and P. polymyxa CS107 [32] have also been proved to be good at acetoin production.…”

Section: Comparison Of Tmp Production Methods From Renewable Feedstocksmentioning

confidence: 99%

Accelerated green process of tetramethylpyrazine production from glucose and diammonium phosphate

Xiao

Hou

Lyu

et al. 2014

Biotechnol Biofuels

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Section: Comparison Of Tmp Production Methods From Renewable Feedstocksmentioning

confidence: 99%

Accelerated green process of tetramethylpyrazine production from glucose and diammonium phosphate

Xiao

Hou

Lyu

et al. 2014

Biotechnol Biofuels

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“…Our previous studies showed that several inorganic salts such as sodium acetate, KH2PO4, MgSO4, MnSO4, FeSO4, and ZnCl2 exhibited positive effects on 2,3-BD production by S. marcescens H30 (Zhang et al 2010a;Sun et al 2012). Sodium acetate as an inducer could increase the transcription level of 2,3-BD pathway in S. marcescens H30 and other 2,3-BD producing microbial strains (Zeng et al 1990;Ji et al 2011;Rao et al 2012), and further result in 2,3-BD accumulation during the fermentation process.…”

Section: Effects Of Inorganic Salts On 23-bd Fermentationmentioning

confidence: 96%

“…During the fermentation process, oxygen supply was shown to be an important factor for 2,3-BD production in previous studies (Zeng et al 1994;Zhang et al 2010a;Ji et al 2011;Sun et al 2012). Therefore, it is necessary for the effect of oxygen supply on 2,3-BD production by S. marcescens H30 under the optimized medium to be investigated.…”

Section: Batch Fermentations With Various Agitation Speedsmentioning

confidence: 99%

“…The optimum nitrogen source was used to carry out further experiments. According to the results from previous studies (Zhang et al 2010a;Sun et al 2012), several inorganic salts (sodium acetate 0-10 g/L, KH2PO4 0-1 g/L, MgSO4 0-0.5 g/L, MnSO4 0-0.5 g/L, FeSO4 0-0.5 g/L, and ZnCl2 0-0.5 g/L) were used to investigate the effects on 2,3-BD production by S. marcescens H30. All the experiments using various inorganic salts were carried out in 50 mL fresh medium containing the SSJ content of 75% and 5 g/L yeast extract for 36 h at 150 rpm and 30 °C in triplicate.…”

Section: Effect Of Nitrogen Sources and Inorganic Salts On 23-bd Promentioning

confidence: 99%

“…Sodium acetate as an inducer could increase the transcription level of 2,3-BD pathway in S. marcescens H30 and other 2,3-BD producing microbial strains (Zeng et al 1990;Ji et al 2011;Rao et al 2012), and further result in 2,3-BD accumulation during the fermentation process. The use of KH2PO4 could provide a phosphorus source for the growth of 2,3-BD producing strains (Zhang et al 2010a;Sun et al 2012). In addition, it has been reported that metal ions including Mg 2+ , Mn 2+ , Fe 2+ , and Zn 2+ were very important for the strain growth and 2,3-BD production by activating the activities of the related enzymes (Zhang et al 2014).…”

Section: Effects Of Inorganic Salts On 23-bd Fermentationmentioning

confidence: 99%

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Utilization of Sweet Sorghum Juice for Efficient 2,3-Butanediol Production by Serratia marcescens H30

Yuan¹,

He²,

Guo³

et al. 2017

BioResources

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Sweet sorghum juice (SSJ) is considered a good carbon source for biorefinery due to its low price and high fermentable-sugar content. In this study, 2,3-butanediol (2,3-BD) production from SSJ by Serratia marcescens H30 was investigated. First, the medium compositions including the contents of SSJ, nitrogen source, and mineral salts were optimized in conical flasks using a single factor and orthogonal design method. Under the optimal conditions, the 2,3-BD concentration reached up to 33.40 g/L. Then the optimized medium was used to perform fermentative experiments in a 5-L bioreactor. In batch experiments, the effects of various agitation speeds on 2,3-BD production were compared. Based on batch process results, an efficient two-stage fermentative control strategy was developed, where the agitation speed was maintained at 300 rpm in the first 12 h and subsequently switched to 200 rpm. About 43.32 g/L 2,3-BD was obtained by using this strategy. Finally, fed-batch fermentation was conducted through feeding the concentrated SSJ and a maximum 2,3-BD concentration of 109.44 g/L with the productivity of 1.40 g/L·h; a yield of 83.02% was achieved. The results showed that SSJ could be used as an economical substrate for efficient 2,3-BD production by S. marcescens H30.

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Rational design of Meso‐2,3‐butanediol dehydrogenase by molecular dynamics simulation and experimental evaluations

Zhang

Wang

et al. 2017

FEBS Letters

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Meso-2,3-butanediol dehydrogenase (meso-2,3-BDH) catalyzes NAD -dependent conversion of meso-2,3-butanediol to acetoin, a crucial external energy storage molecule in fermentive bacteria. In this study, the active tunnel of meso-2,3-BDH was identified. The two short α helixes positioned away from the α4-helix possibly expose the hydrophobic ligand-binding cavity, gating the exit of product and cofactor from the activity pocket. Further MM/GBSA-binding free energy analysis shows that Phe212 and Asn146 function as the key product-release sites. Site-directed mutagenesis experiments targeted to the sites show that the k of Phe212Tyr is enhanced up to (4-8)-fold. The original activity of Asn146Gln is retained, but the activity of Asn146Ala mutation is lost. These results could provide helpful guidance on rational design of short-chain dehydrogenases/reductases.

show abstract

Enhanced acetoin production by Serratia marcescens H32 with expression of a water-forming NADH oxidase

Cited by 96 publications

References 24 publications

Accelerated green process of tetramethylpyrazine production from glucose and diammonium phosphate

Accelerated green process of tetramethylpyrazine production from glucose and diammonium phosphate

Utilization of Sweet Sorghum Juice for Efficient 2,3-Butanediol Production by Serratia marcescens H30

Rational design of Meso‐2,3‐butanediol dehydrogenase by molecular dynamics simulation and experimental evaluations

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