Engraftment of bioengineered three-dimensional scaffold from human amniotic membrane-derived extracellular matrix accelerates ischemic diabetic wound healing

Nasiry, Davood; Khalatbary, Ali Reza; Abdollahifar, Mohammad‐Amin; Amini, Abdollah; Bayat, Mohammad; Noori, Afshin; Piryaei, Abbas

doi:10.1007/s00403-020-02137-3

Cited by 25 publications

(36 citation statements)

References 50 publications

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“…A total of 25 randomly picked micrographs were captured from each sample using a 40× magnification objective lens. The collagen percentage was calculated by dividing the total blue‐coloured area by the total areas of the micrographs using ImageJ software (National Institutes of Health) (Nasiry et al, 2020 ).…”

Section: Methodsmentioning

confidence: 99%

Stereological and molecular studies on the effects of Ferula persica extract on wound healing in rats

Huang¹,

Wang²,

Ebrahimzadeh

et al. 2021

Veterinary Medicine & Sci

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Background: Ferula persica is one of the most important traditional medicinal plants that is used to treat various diseases such as diabetes, backache and rheumatism. The aim of the present study was to evaluate the anti-inflammatory and wound healing potential of F. persica using stereological and molecular methods in experimental models. Methods:In the present study, two wound models (circular excision and linear incision) were used. Male Wistar rats were divided into four groups (n = 16), including control, vehicle treated, treated with F. persica extract ointment (5% w/w) and treated with the reference drug (Madecassol). All the animals were treated topically once a day. The circular and linear wounds were treated for 9 and 17 days, respectively. At the end of the study, samples from wounds area were harvested for histology, stereology, immunohistochemistry and molecular assessments to determine the in vivo healing potential and anti-inflammatory activity. Results:We observed significant recovery in macroscopic evaluation of wound healing in the F. persica extract treated group compared with the control and vehicle treated groups (p < 0.05). Histological and stereological assessments showed complete repair of the epidermal layer, increasing fibroblast cells and collagen density, decreasing inflammatory cells and a remarkable degree of neovascularization by determining length density of blood vessels in the extract group, which were significant as compared to the control and vehicle treated groups (p < 0.05). Expressions of TNF-α and TGF-β were found to be decreased and increased (p > 0.05, p < 0.05, respectively), in the extract treated group as compared to the control and vehicle treated groups. Also, greater COX-2 expression could be detected in the control and vehicle treated groups, which was significantly attenuated in the extract group. Conclusion:Our results confirm that the F. persica extract is a valuable source of antioxidant and anti-inflammatory activity and can allow damaged tissue in wounds to recover markedly.

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Section: Methodsmentioning

confidence: 99%

Stereological and molecular studies on the effects of Ferula persica extract on wound healing in rats

Huang¹,

Wang²,

Ebrahimzadeh

et al. 2021

Veterinary Medicine & Sci

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“…Despite these promising results, dAM dressings have poor mechanical properties and lack long-term stability, which remains a concern for wound healing applications. Nevertheless, several studies have overcome these disadvantages by creating composite scaffolds (Gholipourmalekabadi et al, 2019;Yang et al, 2020;Nasiry et al, 2021). For instance, Yang et al designed a bovine dAMderived composite by utilizing a precursor and a crosslinking agent (chitosan and poly-ethylene glycol diglycidyl ether, respectively) to obtain a sponge-like membrane (CS-BAM) (Yang et al, 2020).…”

Section: Acellular Decellularized Extracellular Matrix From Amniotic ...mentioning

confidence: 99%

Decellularized Tissues for Wound Healing: Towards Closing the Gap Between Scaffold Design and Effective Extracellular Matrix Remodeling

David

Guiza-Arguello

Arango-Rodríguez

et al. 2022

Front. Bioeng. Biotechnol.

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The absence or damage of a tissue is the main cause of most acute or chronic diseases and are one of the appealing challenges that novel therapeutic alternatives have, in order to recover lost functions through tissue regeneration. Chronic cutaneous lesions are the most frequent cause of wounds, being a massive area of regenerative medicine and tissue engineering to have efforts to develop new bioactive medical products that not only allow an appropriate and rapid healing, but also avoid severe complications such as bacterial infections. In tissue repair and regeneration processes, there are several overlapping stages that involve the synergy of cells, the extracellular matrix (ECM) and biomolecules, which coordinate processes of ECM remodeling as well as cell proliferation and differentiation. Although these three components play a crucial role in the wound healing process, the ECM has the function of acting as a biological platform to permit the correct interaction between them. In particular, ECM is a mixture of crosslinked proteins that contain bioactive domains that cells recognize in order to promote migration, proliferation and differentiation. Currently, tissue engineering has employed several synthetic polymers to design bioactive scaffolds to mimic the native ECM, by combining biopolymers with growth factors including collagen and fibrinogen. Among these, decellularized tissues have been proposed as an alternative for reconstructing cutaneous lesions since they maintain the complex protein conformation, providing the required functional domains for cell differentiation. In this review, we present an in-depth discussion of different natural matrixes recently employed for designing novel therapeutic alternatives for treating cutaneous injuries, and overview some future perspectives in this area.

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“…Furthermore, HAM scaffold-treated groups exhibited 1.4-folds increase in volume of epidermis as compared to HAM matrix and normal control groups. The results of collagen deposition study revealed that HAM scaffold-treated groups showed 1.05-folds and 12-folds increase in collagen deposition within 21 days as compared to HAM matrix and normal control groups [ 205 ].…”

Section: Miscellaneousmentioning

confidence: 99%

Recent Progress in Development of Dressings Used for Diabetic Wounds with Special Emphasis on Scaffolds

Awasthi

Gulati

Kumar

et al. 2022

BioMed Research International

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Diabetic wound (DW) is a secondary application of uncontrolled diabetes and affects about 42.2% of diabetics. If the disease is left untreated/uncontrolled, then it may further lead to amputation of organs. In recent years, huge research has been done in the area of wound dressing to have a better maintenance of DW. These include gauze, films, foams or, hydrocolloid-based dressings as well as polysaccharide- and polymer-based dressings. In recent years, scaffolds have played major role as biomaterial for wound dressing due to its tissue regeneration properties as well as fluid absorption capacity. These are three-dimensional polymeric structures formed from polymers that help in tissue rejuvenation. These offer a large surface area to volume ratio to allow cell adhesion and exudate absorbing capacity and antibacterial properties. They also offer a better retention as well as sustained release of drugs that are directly impregnated to the scaffolds or the ones that are loaded in nanocarriers that are impregnated onto scaffolds. The present review comprehensively describes the pathogenesis of DW, various dressings that are used so far for DW, the limitation of currently used wound dressings, role of scaffolds in topical delivery of drugs, materials used for scaffold fabrication, and application of various polymer-based scaffolds for treating DW.

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Engraftment of bioengineered three-dimensional scaffold from human amniotic membrane-derived extracellular matrix accelerates ischemic diabetic wound healing

Cited by 25 publications

References 50 publications

Stereological and molecular studies on the effects of Ferula persica extract on wound healing in rats

Stereological and molecular studies on the effects of Ferula persica extract on wound healing in rats

Decellularized Tissues for Wound Healing: Towards Closing the Gap Between Scaffold Design and Effective Extracellular Matrix Remodeling

Recent Progress in Development of Dressings Used for Diabetic Wounds with Special Emphasis on Scaffolds

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