Engrafted autologous skeletal myoblasts improve myocardial performance in severely infarcted rabbit myocardium

Atkins, B. Zane; Hutcheson, Kelley A.; Hueman, Matthew T.; Cottman, Maisha J.; Meuchel, Jennifer M.; Glower, Donald D.; Taylor, Doris A.

doi:10.1016/s1071-9164(99)91431-8

Cited by 3 publications

(3 citation statements)

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“…However, we have previously injected myoblasts into ligation-induced cardiac injuries and observed similar results to those obtained in this study, suggesting that the model is somewhat comparable. 13 A second question that is not answered by this study is to what extent both cell types affect cardiac remodeling and global contractile function. It has been shown that both bone marrow-derived cells and myoblasts can prevent LV dilatation and hypertrophy.…”

Section: Study Limitationsmentioning

confidence: 89%

Comparison of Intracardiac Cell Transplantation: Autologous Skeletal Myoblasts Versus Bone Marrow Cells

Zenovich¹,

Davis²,

Taylor³

2007

Handbook of Experimental Pharmacology

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Background-Multiple cell types are being proposed for cardiac repair, but side-by-side comparisons are lacking. We tested the hypothesis that intracardiac transplantation of autologous bone marrow-or skeletal muscle-derived progenitor cells improve regional heart function to a similar degree. Methods and Results-Thirty-nine New Zealand White rabbits underwent cryoinjury of the left ventricle and simultaneous hind limb bone marrow aspiration or soleus muscle biopsy. Both muscle and bone marrow cells were expanded in vitro. After 2 weeks, 10 8 skeletal muscle (SM group) or bone marrow-derived progenitor cells (BM group) were injected into the cryoinjured region (SM: nϭ12; BM: nϭ8). Medium alone was injected into the remaining animals (Control: nϭ16). Regional systolic function was measured using micromanometry and sonomicrometry at baseline, before, and 4 weeks after cell injection. Cell treatment resulted in a similar degree of improvement in a derivative of stroke work in the SM and BM groups (Pϭ0.0026 and Pϭ0.0085 versus Control, respectively). No significant difference was seen between BM and SM groups (Pϭ0.9). On histology, engrafted cells were found in all of the cell treated animals. Injected myoblasts formed myotubes or muscle cells throughout the scar that expressed slow and fast myosin heavy chain. A subset of bone marrow cells differentiated toward a myogenic phenotype, as indicated by expression of desmin and ␣-sarcomeric actin in the engrafted areas. Conclusion-Transplantation and myogenic differentiation of bone marrow-derived progenitor cells increased regional systolic heart function after myocardial injury to a similar degree as skeletal myoblasts.

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Section: Study Limitationsmentioning

confidence: 89%

Comparison of Intracardiac Cell Transplantation: Autologous Skeletal Myoblasts Versus Bone Marrow Cells

Zenovich¹,

Davis²,

Taylor³

2007

Handbook of Experimental Pharmacology

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“…Utilization of autologous myogenic stem cells from adult skeletal muscle (satellite cells) for myocardial regeneration in experimental animal was first reported by us in 1989. 8 Since than, many publications from us [8][9][10][11][12][13] and other independent investigators [14][15][16][17][18][19][20][21][22] have demonstrated the feasibility and efficacy of intramyocardial transplantation of satellite cells for myocardial repair and functional improvement in experimental animals. The first clinical case was performed by Menasche et al, 23 since then a number of patients receiving cellular cardiomyoplasty using autologous satellite cells have been announced.…”

Section: Introductionmentioning

confidence: 99%

Clinical Cellular Cardiomyoplasty:

Zhang

Yang

Chen

et al. 2003

Journal of Cardiac Surgery

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Three patients, all with a history of coronary heart disease, underwent coronary artery bypass grafting and implantation of autologous satellite cells. Satellite cells were isolated from muscle biopsies of the right vastus lateralis muscle after enzymatic treatment. While the heart was still under hypothermic cardioplegia, 4 mL of cell suspension divided into approximately 40 doses was injected into the ventricular wall of the ischemic area. Less than 5 minutes were required to complete the cell implantation. All patients survived the procedure, without obvious arrhythmia, had an uneventful recovery, and were discharged from the hospital. At 3 to 4 months follow-up examination, increased left ventricular ejection fraction, decreased left ventricular diastolic diameter, as well as improved ventricular wall thickness and perfusion at the satellite cell implantation sites were observed. Our experience indicated the safety and early benefit of cellular cardiomyoplasty using autologous satellite cells.

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“…In contrast, it differed in the it has been known for a long time that the very incomplete transformation produced by cross-reinnervation of DISCUSSION slow-twitch muscles is in marked contrast to the almost complete transformation usually observed on cross-rein-It has been recently shown that skeletal myoblast transplantation improves in vivo local performances in nervated fast-twitch muscles [(19,20); for review, see (28)]. The data of semiquantitative RT-PCR show two injured or infarcted myocardium (3,42,49). However, few studies have evaluated the effects of myoblast trans-general patterns of MyHC expression.…”

Section: Analysis Of Myhc Isoform Mrna Using Muscle Morphology the Pomentioning

confidence: 99%

Changes in Mass and Performance in Rabbit Muscles after Muscle Damage with or without Transplantation of Primary Satellite Cells

Andalousi¹,

Daussin²,

Micallef

et al. 2002

Cell Transplant

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Changes in morphology, metabolism, myosin heavy chain gene expression, and functional performances in damaged rabbit muscles with or without transplantation of primary satellite cells were investigated. For this purpose, we damaged bilaterally the fast muscle tibialis anterior (TA) with either 1.5 or 2.6 ml cardiotoxin 10 −5 M injections. Primary cultures of satellite cells were autotransplanted unilaterally 5 days after muscle degeneration. Two months postoperation, the masses of damaged TAs, with or without transplantation, were significantly larger than those of the controls. Furthermore, damaged transplanted muscles weighed significantly more than damaged muscles only. The increase in muscle mass was essentially due to increased fiber size. These results were independent of the quantity of cardiotoxin injected into the muscles. Maximal forces were similar in control and 2.6 ml damaged TAs with or without satellite cell transfer. In contrast, 1.5 ml damaged TAs showed a significant decrease in maximal forces that reached the level of controls after transplantation of satellite cells. Fatigue resistance was similar in control and 1.5 ml damaged TAs independently of satellite cell transfer. Fatigue index was significantly higher in 2.6 ml damaged muscles with or without cell transplantation. These changes could be explained in part by muscle metabolism, which shifted towards oxidative activities, and by gene expression of myosin heavy chain isoforms, which presented an increase in type IIa and a decrease in type I and IIb in all damaged muscles with or without cell transfer. Under our experimental conditions, these results show that muscle damage rather than satellite cell transplantation changes muscle metabolism, myosin heavy chain isoform gene expression, and, to a lesser extent, muscle contractile properties. In contrast, muscle weight and fiber size are increased both by muscle damage and by satellite cell transfer.

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Engrafted autologous skeletal myoblasts improve myocardial performance in severely infarcted rabbit myocardium

Cited by 3 publications

References 0 publications

Comparison of Intracardiac Cell Transplantation: Autologous Skeletal Myoblasts Versus Bone Marrow Cells

Comparison of Intracardiac Cell Transplantation: Autologous Skeletal Myoblasts Versus Bone Marrow Cells

Clinical Cellular Cardiomyoplasty:

Changes in Mass and Performance in Rabbit Muscles after Muscle Damage with or without Transplantation of Primary Satellite Cells

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