Engineering virus resistance in agricultural crops

Elzen, Peter J. M. van den; Huisman, Marianne J.; Willink, Dinie Posthumus-Lutke; Jongedijk, Erik; Hoekema, A.; Cornelissen, Ben J. C.

doi:10.1007/bf00025322

Cited by 36 publications

(6 citation statements)

References 40 publications

(65 reference statements)

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“…However, recently produced transgenic plants with enhanced resistance to fungi have been constructed (BROGLIE et al 1991). Engineering of virus resistant plants via coat proteins (VAN DEN ELZEN et al 1989;NEJIDAT et al 1990;TIMMERMAN 1991) and satellite RNA GALLITELLI et al 1991) was more successful.…”

Section: Genomic Librariesmentioning

confidence: 99%

Impact of molecular biology on forest pathology: A literature review

Hubbes

1993

European Journal of Forest Pathology

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Rapid developments in the field of molecular biology have produced a number of very powerful analytical tools by unifying techniques of genetics, biochemistry and cell biology. As reviewed in the present paper, these tools will become important and indispensable in forest pathology studies of the genetic machinery and regulatory mechanisms that control fungal products implicated in the complex tree-pathogen interactions. Understanding these interactions will enhance the development of efficient control methods of forest pathogens.

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Section: Genomic Librariesmentioning

confidence: 99%

Impact of molecular biology on forest pathology: A literature review

Hubbes

1993

European Journal of Forest Pathology

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“…This has led to engineering resistance by construction of transgenic plants that overexpress the viral coat protein and plants that contain replicating copies of a "satellite RNA" (3). The reported applications of antisense technology to the suppression of viral infection in plants have, so far, met with only limited success (4,5). This is probably due to the mode of action of antisense RNA and the viruses chosen as targets (i.e., RNA viruses that replicate in the cytoplasm).…”

mentioning

confidence: 99%

Expression of an antisense viral gene in transgenic tobacco confers resistance to the DNA virus tomato golden mosaic virus.

Day

Bejarano

Buck

et al. 1991

Proc. Natl. Acad. Sci. U.S.A.

140

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Transgenic tobacco plants carrying a genetic cassette including an antisense DNA sequence of the virally encoded ALI gene of the geminivirus tomato golden mosaic virus (TGMV) were constructed; ALI encodes a protein absolutely required for TGMV DNA replication. These genetic cassettes also contained, on the same transcription unit, a gene encoding hygromycin resistance, which allowed selection for concomitant expression of the antisense gene. In transgenic lines, RNA transcripts of the predicted size and strand specificity were detected in antisense plants and sense controls. After infection of plants with TGMV, by agroinoculation, the frequency of symptom development was very significantly reduced in a number of antisense lines and correlated, broadly, with the abundance of antisense RNA transcript and with a reduction in viral DNA harvested from infected leaf tissue. We used an in vitro assay to study viral DNA replication in the absence of cell-to-cell spread; no replication was seen in five of the six antisense lines studied, in contrast to controls.Antisense RNA, complementary to a target RNA (e.g., mRNA), has recently been exploited to artificially suppress gene expression with high specificity (1, 2). Both previously introduced and endogenous genes have successfully been targeted by this technique. The variety of models accounting for the mechanism include antisense RNA-DNA interaction interfering with transcription, rapid degradation of the sense RNA-antisense RNA duplex, a block in the transport of duplex RNA into the cytoplasm, and inhibition oftranslation. Because antisense RNA acts at the transcript level, it is effective against multiple gene copies and, in addition, acts in trans. It would thus seem to be ideal for the suppression of viral infection; viruses invade the cell from outside and multiply to a high copy number.Antisense RNA has been successfully used to suppress viral infection in bacteria and in animal tissue (1). In plants, cross-protection between RNA viral strains has historically been used as a strategy for achieving virus resistance. This has led to engineering resistance by construction of transgenic plants that overexpress the viral coat protein and plants that contain replicating copies of a "satellite RNA" (3). The reported applications of antisense technology to the suppression of viral infection in plants have, so far, met with only limited success (4, 5). This is probably due to the mode of action of antisense RNA and the viruses chosen as targets (i.e., RNA viruses that replicate in the cytoplasm).The single-stranded DNA viruses known as geminiviruses are serious plant pathogens, yet little has been done to engineer plants with resistance. Recently, however, Stanley et al. (6) have shown that transgenic plants containing a defective geminiviral DNA show some decrease in symptom severity following infection. Because geminiviruses replicate in the nucleus, they would seem to be ideal targets to apply antisense technology to engineer resistance. To our knowledge, this p...

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“…CP-mediated protection has been successfully applied to commercial cultivars of potato, a crop affected by a large number of serious viral pathogens [48,52,66,128]. Major potato cultivars have no resistance to many of these viruses.…”

Section: Transformation Of Plants With Coat-protein Coding Sequences:mentioning

confidence: 99%

Genetic engineering of plants for virus resistance

Gadani

Mansky

Medici

et al. 1990

Archives of Virology

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Historically, control of plant virus disease has involved numerous strategies which have often been combined to provide effective durable resistance in the field. In recent years, the dramatic advances obtained in plant molecular virology have enhanced our understanding of viral genome organizations and gene functions. Moreover, genetic engineering of plants for virus resistance has recently provided promising additional strategies for control of virus disease. At present, the most promising of these has been the expression of coat-protein coding sequences in plants transformed with a coat protein gene. Other potential methods include the expression of anti-sense viral transcripts in transgenic plants, the application of artificial anti-sense mediated gene regulation to viral systems, and the expression of viral satellite RNAs, RNAs with endoribonuclease activity, antiviral antibody genes, or human interferon genes in plants.

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Engineering virus resistance in agricultural crops

Cited by 36 publications

References 40 publications

Impact of molecular biology on forest pathology: A literature review

Impact of molecular biology on forest pathology: A literature review

Expression of an antisense viral gene in transgenic tobacco confers resistance to the DNA virus tomato golden mosaic virus.

Genetic engineering of plants for virus resistance

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