Engineering transkingdom signalling in plants to control gene expression in rhizosphere bacteria

Geddes, Barney A.; Ponraj, Paramasivan; Joffrin, Amélie M; Thompson, Amber L.; Christensen, Kirsten E.; Jorrín, Beatriz; Brett, Paul J.; Conway, Stuart J.; Oldroyd, Giles; Poole, Philip S.

doi:10.1038/s41467-019-10882-x

Cited by 105 publications

(79 citation statements)

References 54 publications

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“…Additionally, it allows the monitoring of proline levels across time using a simple plate growth assay. Although lux-based systems come with their own limitations (i.e., dependence on oxygen, ATP or reducing power; Brodl et al 2018), this type of lux-based biosensor has been successfully used for the in vivo monitoring of a number of metabolites including sugars, polyols and organic acids (Pini et al 2017), as well as signalling compounds such as rhizopines (Geddes et al 2019).…”

Section: Discussionmentioning

confidence: 99%

A novel biosensor to monitor proline in pea root exudates and nodules under osmotic stress and recovery

et al. 2020

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Background and aims Plant and bacteria are able to synthesise proline, which acts as a compound to counteract the negative effects of osmotic stresses. Most methodologies rely on the extraction of compounds using destructive methods. This work describes a new proline biosensor that allows the monitoring of proline levels in a non-invasive manner in root exudates and nodules of legume plants. Methods The proline biosensor was constructed by cloning the promoter region of pRL120553, a gene with high levels of induction in the presence of proline, in front of the lux cassette in Rhizobium leguminosarum bv. viciae. Results Free-living assays show that the proline biosensor is sensitive and specific for proline. Proline was detected in both root exudates and nodules of pea plants. The luminescence detected in bacteroids did not show variations during osmotic stress treatments, but significantly increased during recovery. Conclusions This biosensor is a useful tool for the in vivo monitoring of proline levels in root exudates and bacteroids of symbiotic root nodules, and it contributes to our understanding of the metabolic exchange occurring in nodules under abiotic stress conditions.

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Section: Discussionmentioning

confidence: 99%

A novel biosensor to monitor proline in pea root exudates and nodules under osmotic stress and recovery

et al. 2020

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“…The latest investigations confront this challenge from the other side, i.e., by engineering plants to express molecules implicated in this inter‐kingdom communication to modulate rhizospheric bacteria, attenuating possible growth penalties via an inducible expression system (Geddes et al ., 2019). Certainly, the study and the application of biotechnological approaches to optimize plant–bacteria interactions in the rhizosphere will support future development of sustainable agriculture.…”

Section: Interaction Of Microbes With Plants: the Rhizospherementioning

confidence: 99%

More than words: the chemistry behind the interactions in the plant holobiont

Berlanga-Clavero

Molina-Santiago

Vicente

et al. 2020

Environmental Microbiology

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Summary Plants and microbes have evolved sophisticated ways to communicate and coexist. The simplest interactions that occur in plant‐associated habitats, i.e., those involved in disease detection, depend on the production of microbial pathogenic and virulence factors and the host's evolved immunological response. In contrast, microbes can also be beneficial for their host plants in a number of ways, including fighting pathogens and promoting plant growth. In order to clarify the mechanisms directly involved in these various plant–microbe interactions, we must still deepen our understanding of how these interkingdom communication systems, which are constantly modulated by resident microbial activity, are established and, most importantly, how their effects can span physically separated plant compartments. Efforts in this direction have revealed a complex and interconnected network of molecules and associated metabolic pathways that modulate plant–microbe and microbe–microbe communication pathways to regulate diverse ecological responses. Once sufficiently understood, these pathways will be biotechnologically exploitable, for example, in the use of beneficial microbes in sustainable agriculture. The aim of this review is to present the latest findings on the dazzlingly diverse arsenal of molecules that efficiently mediate specific microbe–microbe and microbe–plant communication pathways during plant development and on different plant organs.

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“…Presently, we can successfully engineer cells to sense exogenous inputs [46,47], and control gene expression [48][49][50][51] and communication [52][53][54]. Synthetic interactions within a microbial consortia are typically engineered using the natural quorum sensing system for bacteria [55,56], or different pheromones in fungi [57,58]; but adhesion proteins or ion channels can be also used [59,60].…”

Section: Introductionmentioning

confidence: 99%

Synthetic Biology for Terraformation Lessons from Mars, Earth, and the Microbiome

Conde-Pueyo

Vidiella

Sardanyés

et al. 2020

Life

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What is the potential for synthetic biology as a way of engineering, on a large scale, complex ecosystems? Can it be used to change endangered ecological communities and rescue them to prevent their collapse? What are the best strategies for such ecological engineering paths to succeed? Is it possible to create stable, diverse synthetic ecosystems capable of persisting in closed environments? Can synthetic communities be created to thrive on planets different from ours? These and other questions pervade major future developments within synthetic biology. The goal of engineering ecosystems is plagued with all kinds of technological, scientific and ethic problems. In this paper, we consider the requirements for terraformation, i.e., for changing a given environment to make it hospitable to some given class of life forms. Although the standard use of this term involved strategies for planetary terraformation, it has been recently suggested that this approach could be applied to a very different context: ecological communities within our own planet. As discussed here, this includes multiple scales, from the gut microbiome to the entire biosphere.

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Engineering transkingdom signalling in plants to control gene expression in rhizosphere bacteria

Cited by 105 publications

References 54 publications

A novel biosensor to monitor proline in pea root exudates and nodules under osmotic stress and recovery

A novel biosensor to monitor proline in pea root exudates and nodules under osmotic stress and recovery

More than words: the chemistry behind the interactions in the plant holobiont

Synthetic Biology for Terraformation Lessons from Mars, Earth, and the Microbiome

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