Engineering the Ultrasensitive Visual Whole-Cell Biosensors by Evolved MerR and 5′ UTR for Detection of Ultratrace Mercury
Kaili Zhu,
Dongdong Chen,
Yeshen Cai
et al.
Abstract:The existing mercury whole-cell biosensors (WCBs, parts per billion range) are not able to meet the real-world requirements due to their lack of sensitivity for the detection of ultratrace mercury in the environment. Ultratrace mercury is a potential threat to human health via the food chain. Here, we developed an ultrasensitive mercury WCB by directed evolution of the mercury-responsive transcriptional activator (MerR) sensing module to detect ultratrace mercury. Subsequently, the mutant WCB (m4-1) responding… Show more
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