Engineering the surface of prostate tumor cells and hyaluronan/chitosan multilayer films to modulate cell-substrate adhesion properties

Neto, João Batista Maia Rocha; Neto, Reginaldo Jose Gomes; Bataglioli, Rogério Aparecido; Taketa, Thiago Bezerra; Pimentel, Silvia Borges; Baratti, Mariana Ozello; Costa, Carlos Alberto Rodrigues; Carvalho, Hernandes F.; Beppu, Marisa Masumi

doi:10.1016/j.ijbiomac.2020.04.136

Cited by 11 publications

(17 citation statements)

References 51 publications

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“…This induces changes in the electrical double layer at the interface between the film and PBS solution [44,45]. Indeed, we recently reported the role of hyaluronan and CD44 receptor availability on the modulation of cell-substrate adhesion properties of CHI/HA films [15], which are consistent with the capacitance results. The difference in capacitance induced by exposure to different concentrations of cells is apparent at a fixed frequency (10 Hz, for example), which is evidence of the detection capability of CHI/HA functionalized electrodes.…”

Section: Physicochemical Characterizationsupporting

confidence: 88%

“…The free software PEx-Sensors [30] was used to run the projection technique interactive document mapping (IDMAP) [31][32][33] to evaluate selectivity. Cell adhesion tests with the CHI/HA films assembled onto glass substrates were performed as described in [15] with a 1 h incubation step and a subsequent rinsing step with PBS solution to remove non-adhered cells. The adhered cells were fixed in 2% (w/v) paraformaldehyde (15 min) and washed with DPBS, and then their actin filaments were stained with TRITC-phalloidin (1:500).…”

Section: Detection Of Tumor Cellsmentioning

confidence: 99%

“…Cells adhered to electrodes may be considered as circuit elements, where the impedance changes in a frequency-dependent manner as cells attach to the electrodes [43]. In this study, the electrodes were coated with 3.5 multilayers of CHI/HA using the LbL technique under optimized film assembly conditions (pH 3.0 and 100 mM of NaCl) [15]. A careful electrode selection process based on similar electrical impedance measurements preceded film deposition to avoid noise and interference due to Understanding cell-substrate interactions is vital to developing biomaterials for diagnostics, and this may be achieved by monitoring cell adhesion transduced into electrical signals.…”

Section: Physicochemical Characterizationmentioning

confidence: 99%

“…The overexpression of the CD44H isoform, which contains a specific binding domain to HA in different carcinomas, gliomas, and non-Hodgkin's lymphomas [10], indicates that CD44-HA interactions can be exploited to capture circulating tumor cells (CTC) for diagnostic purposes, though some of the CD44 isoforms do not bind HA [10]. Indeed, HA-functionalized surfaces have been used to capture prostatic cancer cells with biospecific HA-CD44 interactions [14,15]. Detection of CTC is considered a label-free strategy for prostate cancer diagnosis since the number of CTC is a reliable marker to predict tumor response and survival [16,17], even at early stages.…”

Section: Introductionmentioning

confidence: 99%

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Polysaccharide Multilayer Films in Sensors for Detecting Prostate Tumor Cells Based on Hyaluronan-CD44 Interactions

Neto

Soares

Bataglioli

et al. 2020

Cells

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The increasing need for point-of-care diagnosis has sparked the development of label-free sensing platforms, some of which are based on impedance measurements with biological cells. Here, interdigitated electrodes were functionalized with layer-by-layer (LbL) films of hyaluronan (HA) and chitosan (CHI) to detect prostatic tumor cells (PC3 line). The deposition of LbL films was confirmed with atomic force microscopy and polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS), which featured the vibrational modes of the HA top layer capable of interacting specifically with glycoprotein CD44 receptors overexpressed in tumor cells. Though the CHI/HA LbL films cannot be considered as a traditional biosensor due to their limited selectivity, it was possible to distinguish prostate tumor cells in the range from 50 to 600 cells/µL in in vitro experiments with impedance spectroscopy. This was achieved by treating the impedance data with information visualization methods, which confirmed the distinguishing ability of the films by observing the absence of false positives in a series of control experiments. The CD44–HA interactions may, therefore, be exploited in clinical analyses and point-of-care diagnostics for cancer, particularly if computational methods are used to process the data.

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Section: Physicochemical Characterizationsupporting

confidence: 88%

Section: Detection Of Tumor Cellsmentioning

confidence: 99%

Section: Physicochemical Characterizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Polysaccharide Multilayer Films in Sensors for Detecting Prostate Tumor Cells Based on Hyaluronan-CD44 Interactions

Neto

Soares

Bataglioli

et al. 2020

Cells

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“…Observing Figure 4a it is possible to see a grouping of points in the upper-right position of the graphs, showing that the PDMS samples with higher roughness parameters also were the ones that adhered the most cells/mm 2 . Because of Figure 4d, where the points on the X-axis were more spaced than when comparing with the other parameters, the Hurst exponent was thought to be a sig- Rocha Neto and coworkers, where smoother surfaces also resulted in higher PC3 cell adhesion (Rocha Neto et al, 2020).…”

Section: Nano-roughened Pdms Substratesmentioning

confidence: 99%

Surface modification of PDMS substrates for tumour cell adhesion: Influence of roughness parameters

et al. 2020

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Surface properties play a key role in how biomaterials interact with the environment. Surface topography has also been reported to be influential in some research, although its effect is still not well elucidated. In this study, nano-roughened polydimethylsiloxane (PDMS) substrates were developed through a chemically etched intermediate surface. Additionally, PDMS substrates containing 10-30 μm diameter micropillars were functionalized with multilayers of chitosan (CHI) and hyaluronic acid (HA) via layer-by-layer. Such substrates were submitted to cell adhesion assays with PC3 tumour cells. The characterization of these substrates was carried out using an atomic force microscopy (AFM), and some roughness parameters were estimated.Through a statistical description of the topography, we investigated the effects of these surface parameters on PC3 cell adhesion. AFM results indicated a significant modification in the PDMS surface topography and the cell adhesion assays suggest that smoother surfaces induce the PC3 cell adhesion, especially the ones with a high Hurst exponent value. In addition to the AFM analysis, the surface modification of the LbL-functionalized substrates was monitored by contact angle and UV-visible measurements. The improved wettability and the significant Alcian Blue absorbance of the functionalized substrates suggest that the HA/CHI film deposition was successfully accomplished. The LbL functionalization increased the cell capture potential of the PDMS substrates, in which lower diameter micropillars favour the cell adhesion mechanism. Although much work is still needed, the findings advance progress towards the fundamental understanding of the role of nanoscale fractal roughness in cell adhesion and can contribute to the development of new biomaterials with applications in biomedical systems, such as biosensors.

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Polyelectrolyte multilayer films for cancer therapy

Balafouti

Selianitis

Sentoukas

et al. 2023

Novel Platforms for Drug Delivery Applications

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Engineering the surface of prostate tumor cells and hyaluronan/chitosan multilayer films to modulate cell-substrate adhesion properties

Cited by 11 publications

References 51 publications

Polysaccharide Multilayer Films in Sensors for Detecting Prostate Tumor Cells Based on Hyaluronan-CD44 Interactions

Polysaccharide Multilayer Films in Sensors for Detecting Prostate Tumor Cells Based on Hyaluronan-CD44 Interactions

Surface modification of PDMS substrates for tumour cell adhesion: Influence of roughness parameters

Polyelectrolyte multilayer films for cancer therapy

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