Engineering the oleaginous red yeast Rhodotorula glutinis for simultaneous β-carotene and cellulase production

Pi, Hong-Wei; Anandharaj, Marimuthu; Kao, Yi-Ying; Lin, Yu‐Ju; Chang, Jui-Jen; Li, Wen-Hsiung

doi:10.1038/s41598-018-29194-z

Cited by 47 publications

(33 citation statements)

References 54 publications

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“…This indicates that a higher flux is required for carotenoids than what is obtained during biomass production. Overexpression of truncated 3-hydroxy-3-methylglutaryl-CoA reductase from Kluyveromyces marxianus in combination with other genes was shown to increase βcarotene production in Rhodotorula glutinis (Pi et al, 2018). Similarly, also all enzymes of isoprene biosynthetic pathway were predicted to correlate with improved carotenoid production in R. toruloides.…”

Section: Prediction Of Potential Targets For Improved Production Of Cmentioning

confidence: 95%

“…The dimethylallyltranstransferase/geranyltranstransferase (ERG20) is involved in synthesis of farnesyl pyrophosphate (FPP) from geranyl pyrophosphate (GPP) and IPP, which is a direct metabolic precursor of carotenoids as well as ergosterol, heme A, dolichols and prenyl-adducts for prenylated proteins. A recent study has demonstrated that overexpression of the X. dendrorhous geranylgeranyl pyrophosphate (GGPP) synthase (encoded by the BTS1 gene), which is immediately downstream of ERG20, increased 330 carotenoid production in R. glutinis (Pi et al, 2018).…”

Section: Prediction Of Potential Targets For Improved Production Of Cmentioning

confidence: 99%

“…The gene coding for phytoene dehydrogenase (RHTO_04602) has been experimentally verified to be involved in carotenoid biosynthesis (Sun et al, 2017). In addition, overexpression of the X. dendrorhous phytoene desaturase and synthase genes was 335 shown to increase carotenoid yields in R. glutinis (Pi et al, 2018).…”

Section: Prediction Of Potential Targets For Improved Production Of Cmentioning

confidence: 99%

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Genome-scale model of Rhodotorula toruloides metabolism

Tiukova

Prigent

Nielsen

et al. 2019

Preprint

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10The basidiomycete red yeast Rhodotorula toruloides is a promising platform organism for production of biooils. We present rhto-GEM, the first genome-scale model (GEM) of R. toruloides metabolism, that was largely reconstructed using RAVEN toolbox. The model includes 926 genes, 4930 reactions, and 3374 metabolites, while lipid metabolism is described using the SLIMEr formalism allowing direct integration of lipid class and acyl chain experimental distribution data. The simulation results confirmed 15 that the R. toruloides model provides valid growth predictions on glucose, xylose and glycerol, while prediction of genetic engineering targets to increase production of linolenic acid, triacylglycerols and carotenoids highlighted genes that have previously been successfully used to increase production. This renders rtho-GEM valuable for future studies to improve the production of other oleochemicals of industrial relevance including value-added fatty acids and carotenoids, in addition to facilitate system-20 wide omics-data analysis in R. toruloides. Expanding the portfolio of GEMs for lipid accumulating fungi contributes to both understanding of metabolic mechanisms of the oleaginous phenotype but also uncover particularities of the lipid production machinery in R. toruloides.

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Section: Prediction Of Potential Targets For Improved Production Of Cmentioning

confidence: 95%

Section: Prediction Of Potential Targets For Improved Production Of Cmentioning

confidence: 99%

Section: Prediction Of Potential Targets For Improved Production Of Cmentioning

confidence: 99%

See 1 more Smart Citation

Genome-scale model of Rhodotorula toruloides metabolism

Tiukova

Prigent

Nielsen

et al. 2019

Preprint

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“…Consistent with this assumption, Liu et al [79] reported that the codon optimization was the key to Agrobacterium tumefaciensmediated transformation in R. toruloides. Pi et al [80] transformed β-carotene biosynthesis genes (crtI, crtE, crtYB and tHMG1 from X. dendrorhous and Kluyveromyces marxianus) into R. glutinis genome. The transformant P4-10-9-63Y-14b produced β-carotene (27.13 ± 0.66 mg/g) 7-fold higher than the wild type.…”

Section: Use Of Genomic Tools To Characterize Carotenoid Biosynthesismentioning

confidence: 99%

Biosynthetic Pathway of Carotenoids in Rhodotorula and Strategies for Enhanced Their Production

Tang¹,

Wang²,

Jun³

et al. 2019

Journal of Microbiology and Biotechnology

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Rhodotorula is a group of pigment-producing yeasts well known for its intracellular biosynthesis of carotenoids such as β-carotene, γ-carotene, torulene and torularhodin. The great potential of carotenoids in applications in food and feed as well as in health products and cosmetics has generated a market value expected to reach over $2.0 billion by 2022. Due to growing public concern over food safety, the demand for natural carotenoids is rising, and this trend significantly encourages the use of microbial fermentation for natural carotenoid production. This review covers the biological properties of carotenoids and the most recent findings on the carotenoid biosynthetic pathway, as well as strategies for the metabolic engineering methods for the enhancement of carotenoid production by Rhodotorula. The practical approaches to improving carotenoid yields, which have been facilitated by advancements in strain work as well as the optimization of media and fermentation conditions, were summarized respectively.

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“…This indicates that a higher flux is required for carotenoids than what is obtained during biomass production. Overexpression of truncated 3‐hydroxy‐3‐methylglutaryl‐CoA reductase from Kluyveromyces marxianus in combination with other genes was shown to increase β‐carotene production in Rhodotorula glutinis (Pi et al, ). Similarly, also all enzymes of isoprene biosynthetic pathway were predicted to correlate with improved carotenoid production in R. toruloides .…”

Section: Resultsmentioning

confidence: 99%

Genome‐scale model of Rhodotorula toruloides metabolism

Tiukova

Prigent

Nielsen

et al. 2019

Biotech & Bioengineering

View full text Add to dashboard Cite

The basidiomycete red yeast Rhodotorula toruloides is a promising platform organism for production of biooils. We present rhto-GEM, the first genome-scale model (GEM) of R. toruloides metabolism, that was largely reconstructed using RAVEN toolbox. The model includes 852 genes, 2,731 reactions, and 2,277 metabolites, while lipid metabolism is described using the SLIMEr formalism allowing direct integration of lipid class and acyl chain experimental distribution data. The simulation results confirmed that the R. toruloides model provides valid growth predictions on glucose, xylose, and glycerol, while prediction of genetic engineering targets to increase production of linolenic acid, triacylglycerols, and carotenoids identified genes-some of which have previously been engineered to successfully increase production. This renders rtho-GEM valuable for future studies to improve the production of other oleochemicals of industrial relevance including value-added fatty acids and carotenoids, in addition to facilitate system-wide omics-data analysis in R. toruloides.Expanding the portfolio of GEMs for lipid-accumulating fungi contributes to both understanding of metabolic mechanisms of the oleaginous phenotype but also uncover particularities of the lipid production machinery in R. toruloides. K E Y W O R D Sgenome-scale model, metabolism, Rhodotorula toruloides, yeast

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Engineering the oleaginous red yeast Rhodotorula glutinis for simultaneous β-carotene and cellulase production

Cited by 47 publications

References 54 publications

Genome-scale model of Rhodotorula toruloides metabolism

Genome-scale model of Rhodotorula toruloides metabolism

Biosynthetic Pathway of Carotenoids in Rhodotorula and Strategies for Enhanced Their Production

Genome‐scale model of Rhodotorula toruloides metabolism

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