Engineering the native methylotrophs for the bioconversion of methanol to value-added chemicals: current status and future perspectives

Wang, Jing; Qin, Ruirui; Guo, Yuanke; Ma, Chen; Wang, Xin; Chen, Kequan; Ouyang, Pingkai

doi:10.1016/j.gce.2022.10.005

Cited by 10 publications

(4 citation statements)

References 156 publications

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“…More efficient genetic tools should be developed for the metabolic engineering of native methylotrophs. The gas-liquid mass transfer limitations must be overcome to meet the high requirements of O 2 for the oxidation of methanol [ [9] , [10] , [11] ]. Although proof-of-concept production of different chemicals has been demonstrated using engineered native methylotrophs, recombinant protein production from methylotrophic yeasts and amino acid production from methylotrophic bacteria are high enough to compete with sugar-based bioprocesses.…”

Section: Discussion and Future Perspectivesmentioning

confidence: 99%

“…In recent years, synthetic methylotrophs have gained much attention for the conversion of model microorganisms such as E. coli, Corynebacterium glutamicum , and Saccharomyces cerevisiae into methanol-utilizing microbes. Although the growth rates and product yields of synthetic methylotrophs are much lower than those of native methylotrophs, their potential is sufficient to be investigated for the production of fuels and chemicals [ 10 ]. In this review, we comprehensively discuss the potential and limitations of various native and synthetic methylotrophs for the production of fuels and chemicals.…”

Section: Introductionmentioning

confidence: 99%

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Methanol-based biomanufacturing of fuels and chemicals using native and synthetic methylotrophs

Sarwar

Lee

2023

Synthetic and Systems Biotechnology

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Section: Discussion and Future Perspectivesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Methanol-based biomanufacturing of fuels and chemicals using native and synthetic methylotrophs

Sarwar

Lee

2023

Synthetic and Systems Biotechnology

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“…Regarding methanol, its assimilation in microorganisms typically employs two approaches: integration of methanol into the WL pathway via methanol methyltransferase, and oxidization of methanol to formaldehyde and formate, which are further assimilated through downstream pathways (Bae et al 2022 ; Litty et al 2022 ). The former approach has been identified in a representative acetogenic bacterium, Acetobacterium woodii (Litty et al 2022 ; Wang et al 2023 ). Whether these two approaches both function in the methanol metabolism in M. thermoacetica remains unclear.…”

Section: Broad Carbon Substrate Range Of M Thermoaceticamentioning

confidence: 99%

Thermophilic Moorella thermoacetica as a platform microorganism for C1 gas utilization: physiology, engineering, and applications

Jia,

Deng,

et al. 2023

Bioresour. Bioprocess.

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In the context of the rapid development of low-carbon economy, there has been increasing interest in utilizing naturally abundant and cost-effective one-carbon (C1) substrates for sustainable production of chemicals and fuels. Moorella thermoacetica, a model acetogenic bacterium, has attracted significant attention due to its ability to utilize carbon dioxide (CO2) and carbon monoxide (CO) via the Wood–Ljungdahl (WL) pathway, thereby showing great potential for the utilization of C1 gases. However, natural strains of M. thermoacetica are not yet fully suitable for industrial applications due to their limitations in carbon assimilation and conversion efficiency as well as limited product range. Over the past decade, progresses have been made in the development of genetic tools for M. thermoacetica, accelerating the understanding and modification of this acetogen. Here, we summarize the physiological and metabolic characteristics of M. thermoacetica and review the recent advances in engineering this bacterium. Finally, we propose the future directions for exploring the real potential of M. thermoacetica in industrial applications.

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“…Expanding these rates to produce target compounds is an insistent need. While the limited availability of genetic tools poses a challenge, the strides made in synthetic biology now enable the development of these tools to engineer native methylotrophs [168]. For example, B. methanolicus was modified to generate L-lysine by implementing the CRISPRi system [169].…”

Section: Methanolmentioning

confidence: 99%

Perspectives for Using CO2 as a Feedstock for Biomanufacturing of Fuels and Chemicals

Kurt,

Qin,

Williams

et al. 2023

Bioengineering

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Microbial cell factories offer an eco-friendly alternative for transforming raw materials into commercially valuable products because of their reduced carbon impact compared to conventional industrial procedures. These systems often depend on lignocellulosic feedstocks, mainly pentose and hexose sugars. One major hurdle when utilizing these sugars, especially glucose, is balancing carbon allocation to satisfy energy, cofactor, and other essential component needs for cellular proliferation while maintaining a robust yield. Nearly half or more of this carbon is inevitably lost as CO2 during the biosynthesis of regular metabolic necessities. This loss lowers the production yield and compromises the benefit of reducing greenhouse gas emissions—a fundamental advantage of biomanufacturing. This review paper posits the perspectives of using CO2 from the atmosphere, industrial wastes, or the exhausted gases generated in microbial fermentation as a feedstock for biomanufacturing. Achieving the carbon-neutral or -negative goals is addressed under two main strategies. The one-step strategy uses novel metabolic pathway design and engineering approaches to directly fix the CO2 toward the synthesis of the desired products. Due to the limitation of the yield and efficiency in one-step fixation, the two-step strategy aims to integrate firstly the electrochemical conversion of the exhausted CO2 into C1/C2 products such as formate, methanol, acetate, and ethanol, and a second fermentation process to utilize the CO2-derived C1/C2 chemicals or co-utilize C5/C6 sugars and C1/C2 chemicals for product formation. The potential and challenges of using CO2 as a feedstock for future biomanufacturing of fuels and chemicals are also discussed.

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Engineering the native methylotrophs for the bioconversion of methanol to value-added chemicals: current status and future perspectives

Cited by 10 publications

References 156 publications

Methanol-based biomanufacturing of fuels and chemicals using native and synthetic methylotrophs

Methanol-based biomanufacturing of fuels and chemicals using native and synthetic methylotrophs

Thermophilic Moorella thermoacetica as a platform microorganism for C1 gas utilization: physiology, engineering, and applications

Perspectives for Using CO2 as a Feedstock for Biomanufacturing of Fuels and Chemicals

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