Engineering the bacterial endophyte Burkholderia pyrrocinia JK-SH007 for the control of lepidoptera larvae by introducing the cry218 genes of Bacillus thuringiensis

Yang, Li; Wu, Choufei; Zhong, Xing; Gao, Bingli; Zhang, Liqin

doi:10.1080/13102818.2017.1379361

Cited by 24 publications

(14 citation statements)

References 17 publications

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“…cynodontis , which colonizes the xylem of several plant species, was transgenically modified to express the Bacillus thuringiensis gene encoding endotoxin for control of insects . In another example, an endophytic Burkholderia pyrrocinia JK‐SH007 was transformed with the Bt endotoxin gene to express the insecticidal protein against the second stage of Bombyx mori instar silkworms . Further, an endophytic Pseudomonas putida WCS358r was modified with an antifungal gene and introduced into wheat with a resultant reduction in fungal populations in soil, including pathogenic Fusarium spp .…”

Section: Applications Of Endophytes In Agriculturementioning

confidence: 99%

“…53 Further, an endophytic Pseudomonas putida WCS358r was modified with an antifungal gene and introduced into wheat with a resultant reduction in fungal populations in soil, including pathogenic Fusarium spp. 53 It seems likely that future efforts to manage crops could involve the exploitation of transgenically modified endophytes. However, due to the mobility of endophytic microbes containment of the microbes to specific plants may be difficult or impossible.…”

Section: Transgenic Endophytesmentioning

confidence: 99%

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Review: Endophytic microbes and their potential applications in crop management

White

Kingsley

Zhang

et al. 2019

Pest Management Science

293

200

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Endophytes are microbes (mostly bacteria and fungi) present asymptomatically in plants. Endophytic microbes are often functional in that they may carry nutrients from the soil into plants, modulate plant development, increase stress tolerance of plants, suppress virulence in pathogens, increase disease resistance in plants, and suppress development of competitor plant species. Endophytic microbes have been shown to: (i) obtain nutrients in soils and transfer nutrients to plants in the rhizophagy cycle and other nutrient‐transfer symbioses; (ii) increase plant growth and development; (iii) reduce oxidative stress of hosts; (iv) protect plants from disease; (v) deter feeding by herbivores; and (vi) suppress growth of competitor plant species. Because of the effective functions of endophytic microbes, we suggest that endophytic microbes may significantly reduce use of agrochemicals (fertilizers, fungicides, insecticides, and herbicides) in the cultivation of crop plants. The loss of endophytic microbes from crop plants during domestication and long‐term cultivation could be remedied by transfer of endophytes from wild relatives of crops to crop species. Increasing atmospheric carbon dioxide levels could reduce the efficiency of the rhizophagy cycle due to repression of reactive oxygen used to extract nutrients from microbes in roots. © 2019 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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Section: Applications Of Endophytes In Agriculturementioning

confidence: 99%

Section: Transgenic Endophytesmentioning

confidence: 99%

Review: Endophytic microbes and their potential applications in crop management

White

Kingsley

Zhang

et al. 2019

Pest Management Science

293

200

View full text Add to dashboard Cite

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“…Currently, some strains of B. megaterium, P. fluorescens, E. coli, and B. subtilis [ 27 ] have been genetically modified via insertion of Cry toxin genes. Importantly, insertion of the Btcry218 gene of Bacillus thuringiensis into the genome of the Burkholderia pyrrocinia JK-SH007 strain promotes 80% mortality of silkworms Bombyx mori larvae 24 h after eating mulberry leaves immersed in B. pyrrocinia JK-SH007 suspension [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

Endophytic Strain Bacillus subtilis 26DCryChS Producing Cry1Ia Toxin from Bacillus thuringiensis Promotes Multifaceted Potato Defense against Phytophthora infestans (Mont.) de Bary and Pest Leptinotarsa decemlineata Say

Sorokan

Benkovskaya

Бурханова

et al. 2020

Plants

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Novel properties of a previously obtained Bacillus subtilis 26DCryChS strain are described. The B. subtilis 26DCryChS strain is able to produce Cry1Ia δ-endotoxin from B. thuringiensis B-5351 and to exist in internal plant tissues of potato plants in the same manner as the endophytic B. subtilis 26D source strain (487 ± 53 and 420 ± 63 CFU*103/g, respectively). B. subtilis 26DCryChS, as much as the original B. subtilis 26D strain, inhibited mycelium growth of oomycete Phytophthora infestans (Mont.) de Bary and reduced late blight symptoms development on plants by 35% compared with non-treated ones, as well as showed insecticidal activity against Leptinotarsa decemlineata. Production of the fluorescent GFP protein in the B. subtilis 26D genome allowed visualizing the endophytes around damaged sites on beetle intestines. Bacillus strains under investigation induced systemic resistance to P. infestans and L. decemlineata through the activation of the transcription of PR genes in potato plants. Thus, the B. subtilis 26DCryChS strain was able to induce transcription of jasmonate-dependent genes and acquired the ability to promote transcription of a salicylate-dependent gene (PR1) in plants infected with the late blight agent and damaged by Colorado potato beetle larvae. The B. subtilis 26DCryChS strain could be put forward as a modern approach for biocontrol agents design.

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“…Twenty larvae were incubated in each culture tube for each replicate at 25±1°C [14] . All insects were checked for viability after 4 days.…”

Section: Fisher Scienti C Usa)mentioning

confidence: 99%

Production of the 13C/15N single-labeled insecticidal protein Cry1Ab/Ac for the assessment of metabolic fate using recombinant Escherichia coli

Wang

Sun

et al. 2020

Preprint

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Stable isotope-labeled Cry1Ab/Ac protein is necessary for the metabolic study of exogenous insecticidal protein in soil using the stable isotope labeling technique, but no recombinant expression protocols for this protein have been reported. The artificially synthesized gene Cry1Ab/Ac of Bt rice Huahui No. 1, which obtained the safety certificate in China, was subcloned into pUC57 in this study, and the expression vector pET-28a-CryAb/Ac was constructed and transformed into Escherichia coli BL21 (DE3) competent cells. Next, 0.2 mM IPTG was added to these cells and cultured at 37°C for 4 h to induce the expressed protein to form inclusion bodies in the presence of M9 medium containing either [U-13C] glucose (5% 13C-enriched) or [15N] ammonium chloride (5% 15N-enriched). Then Cry inclusion bodies were dissolved in urea and purified by Ni column affinity chromatography under denaturing conditions, renatured by dialysis, and further detected by SDS-PAGE and Western blot. The purities of 13C/15N-labeled Cry proteins were each 99%, the amounts of which were 12.6 mg/L and 8.8 mg/L. The δ 13C and δ 15N values of 13C-labeled Cry protein and 15N-labeled Cry protein were 3268.68‰ and 2854.28‰, respectively. An insecticidal test showed that the prokaryotic expression of Cry1Ab/Ac protein had strong insecticidal activity. The stable isotope-labeled insecticidal Cry proteins produced for the first time in this study will provide an experimental basis for future metabolic studies of Cry protein in soil and the characteristics of nitrogen (N) and carbon (C) transformation. The findings will also provide a reference and basis for elucidating the environmental behaviors and ecological effects of Cry plants and expressed products.

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Engineering the bacterial endophyte Burkholderia pyrrocinia JK-SH007 for the control of lepidoptera larvae by introducing the cry218 genes of Bacillus thuringiensis

Cited by 24 publications

References 17 publications

Review: Endophytic microbes and their potential applications in crop management

Review: Endophytic microbes and their potential applications in crop management

Endophytic Strain Bacillus subtilis 26DCryChS Producing Cry1Ia Toxin from Bacillus thuringiensis Promotes Multifaceted Potato Defense against Phytophthora infestans (Mont.) de Bary and Pest Leptinotarsa decemlineata Say

Production of the 13C/15N single-labeled insecticidal protein Cry1Ab/Ac for the assessment of metabolic fate using recombinant Escherichia coli

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