Engineering Synthetic Microbial Communities through Selective Biofilm Cultivation Device for the Production of Fermented Beverages

Ly, Sarann; Kakahi, Fatemeh Bajoul; Phat, C; Mith, Hasika; Fifani, Barbara; Kenne, Thierry; Fauconnier, Marie‐Laure; Delvigne, Frank

doi:10.20944/preprints201906.0272.v1

Cited by 4 publications

(1 citation statement)

References 33 publications

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“…Improved strains of filamentous fungi are widely used in the biotechnology industry for recycling of secondary raw materials [1][2][3] as biosorbents [4], in fermentation of cheese [5], wine [6,7], and other food products [8,9], as well as for the production of enzymes [10][11][12][13], organic acids [14,15], secondary metabolites (SMs) [16,17], or for steroid transformation [18,19]. There are four main tools for fungal strain improvement: (1) sexual crossing [20,21], (2) somatic crossing (including parasexual recombination [22]), (3) random mutagenesis by physical or/and chemical mutagens and screening [23,24], and (4) genetic engineering [25][26][27].…”

Section: Introductionmentioning

confidence: 99%

Random Mutagenesis of Filamentous Fungi Strains for High-Yield Production of Secondary Metabolites: The Role of Polyamines

Жгун¹

2021

Genotoxicity and Mutagenicity - Mechanisms and Test Methods

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A filamentous fungus (also called molds or moldy fungus) is a taxonomically diverse organism from phylum Zygomycota and Ascomycota with filamentous hyphae and has the ability to produce airborne spores or conidia. Currently, more than 70,000 molds are known, and some of them contain unique and unusual biochemical pathways. A number of products from such pathways, especially, the secondary metabolite (SM) pathways are used as important pharmaceuticals, including antibiotics, statins, and immunodepresants. Under different conditions, the individual species can produce more than 100 SM. The strain improvement programs lead to high yielding in target SM and significant reduction of spin-off products. The main tool for the strain improvement of filamentous fungi is random mutagenesis and screening. The majority of industrial overproducing SM strains were developed with the help of such technique over the past 50–70 years; the yield of the target SM increased by 100- to 1000-fold or more. Moreover, most of the strains have reached their technological limit of improvement. A new round of mutagenesis has not increased overproduction. Recently, it was shown that that the addition of exogenous polyamines may increase the production of such improved strains of filamentous fungi. The possible molecular mechanism of this phenomenon and its biotechnological applications are discussed.

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Section: Introductionmentioning

confidence: 99%

Random Mutagenesis of Filamentous Fungi Strains for High-Yield Production of Secondary Metabolites: The Role of Polyamines

Жгун¹

2021

Genotoxicity and Mutagenicity - Mechanisms and Test Methods

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Sherry Wines: Worldwide Production, Chemical Composition and Screening Conception for Flor Yeasts

Avdanina

Zhgun

2022

Fermentation

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The manufacturing of sherry wines is a unique, carefully regulated process, from harvesting to quality control of the finished product, involving dynamic biological aging in a “criadera-solera” system or some other techniques. Specialized “flor” strains of the yeast Saccharomyces cerevisiae play the central role in the sherry manufacturing process. As a result, sherry wines have a characteristic and unique chemical composition that determines their organoleptic properties (such as color, odor, and taste) and distinguishes them from all other types of wine. The use of modern methods of genetics and biotechnology contributes to a deep understanding of the microbiology of sherry production and allows us to define a new methodology for breeding valuable flor strains. This review discusses the main sherry-producing regions and the chemical composition of sherry wines, as well as genetic, oenological, and other selective markers for flor strains that can be used for screening novel candidates that are promising for sherry production among environmental isolates.

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Biofilm Mode of Cultivation Leads to an Improvement of the Entomotoxic Patterns of Two Aspergillus Species

et al. 2020

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Two fungi, i.e., Aspergillus flavus Link and Aspergillus oryzae (Ahlb.) E. Cohn, were cultivated according to two methodologies, namely submerged and biofilm cultures with the primary aim to use their secondary metabolites the supernatant CL50, and CL90 varied between 1.3% (v/v) to 12.7% (v/v) for incubation times from 24 to 72 h. While the A. flavus supernatant entomotoxicity was higher than this of A. oryzae, the biofilm culture application increased the efficiency of the former. Proteomic analysis of the supernatants revealed discrepancies among the two species and modes of cultivation. Furthermore, the secondary metabolite profiles of both Aspergillus cultures were verified. Aspergillic acid, beta-cyclopiazonic acid, cyclopiazonic acid, ferrineospergillin, flavacol, and spermadin A were most predominant. Generally, these secondary metabolites were present in higher concentrations in the supernatants of A. flavus and biofilm cultures. These molecular identifications correlated positively with entomotoxic activity. Noteworthy, the absence of carcinogenic aflatoxins was remarkable, and it will allow further valorization to produce A. flavus to develop potential biopesticides.

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Engineering Synthetic Microbial Communities through Selective Biofilm Cultivation Device for the Production of Fermented Beverages

Cited by 4 publications

References 33 publications

Random Mutagenesis of Filamentous Fungi Strains for High-Yield Production of Secondary Metabolites: The Role of Polyamines

Random Mutagenesis of Filamentous Fungi Strains for High-Yield Production of Secondary Metabolites: The Role of Polyamines

Sherry Wines: Worldwide Production, Chemical Composition and Screening Conception for Flor Yeasts

Biofilm Mode of Cultivation Leads to an Improvement of the Entomotoxic Patterns of Two Aspergillus Species

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