Engineering Strategies for Peripheral Nerve Repair

Hudson, Terry W.; Evans, Gregory R. D.; Schmidt, Christine E.

doi:10.1016/s0030-5898(05)70166-8

Cited by 175 publications

(174 citation statements)

References 72 publications

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“…Proposals to overcome these difficulties involve the use of systems composed of biocompatible structures acting as a provisory extracellular matrix, exogenous or endogenous healthy cells transplanted within scaffolds, and different cytokines or molecular cues to provide a regenerative guide [8]. A number of studies aiming at the repair of nerve or brain tissue have been conducted with promising results, especially those which used cylindrical or tubular structures to mimic nervous tracts [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Schwann-cell cylinders grown inside hyaluronic-acid tubular scaffolds with gradient porosity

et al. 2016

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Cell transplantation therapies in the nervous system are frequently hampered by glial scarring and cell drain from the damaged site, among others. To improve this situation, new biomaterials may be of help. Here, novel single-channel tubular conduits based on hyaluronic acid (HA) with and without poly-L-lactide acid fibers in their lumen were fabricated. Rat Schwann cells were seeded within the conduits and cultured for 10 days.The conduits possessed a three-layered porous structure that impeded the leakage of the cells seeded in their interior and made them impervious to cell invasion from the exterior, while allowing free transport of nutrients and other molecules needed for cell survival. The channel's surface acted as a template for the formation of a cylindrical sheath-like tapestry of Schwann cells continuously spanning the whole length of the lumen. Schwann-cell tubes having a diameter of around 0.5 mm and variable lengths can thus be generated. This structure is not found in nature and represents a truly engineered tissue, the outcome of the specific cell-material interactions. The conduits might be useful to sustain and protect cells for transplantation, and the biohybrids here described, together with neuronal precursors, might be of help in building bridges across significant distances in the central and peripheral nervous system.

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Section: Introductionmentioning

confidence: 99%

Schwann-cell cylinders grown inside hyaluronic-acid tubular scaffolds with gradient porosity

et al. 2016

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“…A variety of natural materials have been used such as vein, laminin, fibronectin, and collagen (Wang et al, 1994). Although natural materials are ideal, there are some inherent difficulties and these include undesirable immune responses, the potential use of immunosuppressive drugs, and batch to batch variation (Hudson et al, 1999). In an attempt to use natural material, nerve grafts have been stored for prolonged periods of time (Evans et al, 1995).…”

Section: Scaffoldmentioning

confidence: 99%

Peripheral nerve injury: A review and approach to tissue engineered constructs

2001

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Eleven thousand Americans each year are affected by paralysis, a devastating injury that possesses associated annual costs of $7 billion (American Paralysis Association, 1997). Currently, there is no effective treatment for damage to the central nervous system (CNS), and acute spinal cord injury has been extraordinarily resistant to treatment. Compared to spinal cord injury, damage to peripheral nerves is considerably more common. In 1995, there were in excess of 50,000 peripheral nerve repair procedures performed. (National Center for Health Statistics based on Classification of Diseases, 9th Revision, Clinical Modification for the following categories: ICD-9 CM Code: 04.3, 04.5, 04.6, 04.7). These data, however, probably underestimate the number of nerve injuries appreciated, as not all surgical or traumatic lesions can be repaired. Further, intraabodominal procedures may add to the number of neurologic injuries by damage to the autonomic system through tumor resection. For example, studies assessing the outcome of impotency following radical prostatectomy demonstrated 212 of 503 previously potent men (42%) suffered impotency when partial or complete resection of one or both cavernosal nerve(s). This impotency rate decreased to 24% when the nerves were left intact (Quinlan et al., J.

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“…A large gap in the peripheral nerve will not permit effective regeneration unless a grafting conduit is used to bridge the defect (2). The current gold standard for the clinical treatment of severe peripheral nerve damage involves using an autologous nerve to bridge the defect in injured nerve (3). This method has been shown to be effective, but has the several disadvantages, including an extra incision for removal of a healthy sensory nerve ultimately resulting in a sensory deficit at the donor site (4)(5), also neuroma and scar tissue are common (6).…”

Section: Introductionmentioning

confidence: 99%

“…This method has been shown to be effective, but has the several disadvantages, including an extra incision for removal of a healthy sensory nerve ultimately resulting in a sensory deficit at the donor site (4)(5), also neuroma and scar tissue are common (6). In an effort to overcome these limitations (7), the use of nerve guidance channels (NGCs) to bridge the gap between severed nerve ends is being extensively explored (3). Currently a vast amount of research was being pursued to engineer the ideal NGCs that can promote both sensory and motor functions (8).…”

Section: Introductionmentioning

confidence: 99%

Comparison Outcome of Nerve Regeneration across an Eggshell Membrane Guidance Channel and Autograft

Farjah

Saberi

2016

Caspian.J.Neurol.Sci

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Background: Peripheral nerves may be damaged during an injury and its current standard treatment is using an autologous nerve. Objectives: The purpose of this experimental study is to evaluate and compare the histological results of nerve regeneration after using the eggshell membrane (ESM) guidance channel with autograft. Materials and Methods: Thirty adult male rats were divided into three experimental groups: ESM guidance channel, autograft, and sham surgery. The decalcifying membrane of egg rotated over the Teflon mandrel and dried at 37°C. A 10 mm nerve segment of left sciatic nerve was cut and removed. In ESM group, the ends of the sciatic nerve were telescoped into the nerve guides. In autograft group, the nerve segment was reversed and used as an autologous nerve graft. At 90 days after surgery, all animals were evaluated by histological and immunohistochemical assessment. Results: The diameters of regenerated myelinated fibers were 5.24±2.14 µm for the ESM group, and 5.89±2.99 µm for the autograft group. The number of myelinated axons regenerated in the ESM group (9824±218 nerve fibers) was significantly greater than autograft group (7865±314 nerve fibers) (p<0.05).Conclusion: These findings demonstrate that ESM effectively enhances nerve regeneration in injured rat sciatic nerve.

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Engineering Strategies for Peripheral Nerve Repair

Cited by 175 publications

References 72 publications

Schwann-cell cylinders grown inside hyaluronic-acid tubular scaffolds with gradient porosity

Schwann-cell cylinders grown inside hyaluronic-acid tubular scaffolds with gradient porosity

Peripheral nerve injury: A review and approach to tissue engineered constructs

Comparison Outcome of Nerve Regeneration across an Eggshell Membrane Guidance Channel and Autograft

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