Engineering status of protein for improving microbial cell factories

Zhou, Pei; Gao, Cong; Song, Wei; Wei, Wanqing; Wu, Jing; Liu, Liming; Chen, Xiulai

doi:10.1016/j.biotechadv.2023.108282

Cited by 6 publications

(1 citation statement)

References 140 publications

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“…Although chemical synthesis is a viable strategy, its high cost makes it unsuitable for large-scale industrial production [ 6 ]. Techniques for producing recombinant proteins in appropriate cell factories have a long-established history, and biotechnological methods are regarded as “green” and safer than chemical synthesis [ 8 ]. Since 1999, researchers have attempted to express class IIa bacteriocins in a heterologous host.…”

Section: Introductionmentioning

confidence: 99%

The Progress of the Biotechnological Production of Class IIa Bacteriocins in Various Cell Factories and Its Future Challenges

Wang,

Shang,

Huang

et al. 2024

IJMS

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Class IIa bacteriocins produced in lactic acid bacteria are short cationic peptides with antimicrobial activity. In the search for new biopreservation agents, class IIa bacteriocins are considered to be the best potential candidates, not only due to their large abundance but also because of their high biological activity and excellent thermal stability. However, regulated by the biosynthetic regulatory system, the natural class IIa bacteriocin yield is low, and the extraction process is complicated. The biotechnological production of class IIa bacteriocins in various cell factories has been attempted to improve this situation. In this review, we focus on the application of biotechnological routes for class IIa bacteriocin production. The drawbacks and improvements in the production of class IIa bacteriocins in various cell factories are discussed. Furthermore, we present the main challenge of class IIa bacteriocins, focusing on increasing their production by constructing suitable cell factories. Recombinant bacteriocins have made considerable progress from inclusion body formation, dissolved form and low antibacterial activity to yield recovery. The development of prospective cell factories for the biotechnological production of bacteriocins is still required, which may facilitate the application of bacteriocins in the food industry.

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Section: Introductionmentioning

confidence: 99%