Bisphenol A (BPA)
has been widely used as a plasticizer in the
production of synthetic polymers, such as those used in food storage
containers and bottles. However, BPA interferes with endocrine systems,
causing carcinogenicity, immunotoxicity, and embryotoxicity. Biological
water treatment processes scarcely remove BPA, owing to the poor BPA
degradation ability and efficiency of the applied microorganisms. Shewanella oneidensis has been studied and used for the
biodegradation process in wastewater treatment because of its excellent
extracellular electron transfer properties. In this work, we engineered S. oneidensis MR1 to enable BPA degradation by producing
ferredoxin (Fdbisd) and cytochrome P450 (P450bisd) originating from Sphingomonas bisphenolicum AO1.
The engineered S. oneidensis exhibited a higher BPA
degradation efficiency than Escherichia coli producing
the same enzymes. The endogenous ferredoxin and ferredoxin reductase
of S. oneidensis participated in BPA degradation,
and overexpression of mtrC, omcA, and So0521, which encode S. oneidensis cytochromes, decreased BPA. We developed BPA-degrading S.
oneidensis biofilms. This study provides insights into biocatalyst
utilization for the biological degradation of toxic organic compounds.