Engineering S. oneidensis for Performance Improvement of Microbial Fuel Cell—a Mini Review

Leung, Dexter Hoi Long; Lim, Yin Sze; Uma, Kasimayan; Pan, Guan‐Ting; Lin, Ja-Hon; Chong, Siewhui; Yang, Thomas C.-K.

doi:10.1007/s12010-020-03469-6

Cited by 32 publications

(12 citation statements)

References 76 publications

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“…44 DcgZ (YdeH), a two-guanylate cyclase in E. coli, catalyzes the biosynthesis of c-di-GMP. 32,43 Thus, the overproduction of DcgZ in S. oneidensis strain increased the biofilm formation (Figure 5). However, CsgD overproduction together with the production of BPA degrading enzymes did not increase the biofilm formation.…”

Section: Discussionmentioning

confidence: 93%

“…Increasing the biosynthesis of c-di-GMP, an intracellular signaling molecule, improves biofilm formation, because c-di-GMP promotes the expression of adherent matrix components . DcgZ (YdeH), a two-guanylate cyclase in E. coli , catalyzes the biosynthesis of c-di-GMP. , Thus, the overproduction of DcgZ in S. oneidensis strain increased the biofilm formation (Figure ). However, CsgD overproduction together with the production of BPA degrading enzymes did not increase the biofilm formation.…”

Section: Discussionmentioning

confidence: 99%

“…However, S. oneidensis exhibits excellent EET ability. In S. oneidensis MR1, electrons derived from the oxidation of carbon sources and the electron donor flow from NADH through intracellular menaquinol pools and a metal-reducing (Mtr) pathway. ,, The electrons subsequently transfer to extracellular electron acceptors in the contact-based EET pathway through c-type cytochromes or soluble electron shuttle-mediated EET pathways using flavins as electron shuttles and bound cofactors for OmcA and MtrC. , P450 bisd is a member of the cytochrome P450s, a superfamily of enzymes that contain heme as a cofactor and function as monooxygenases by using ferredoxin and a ferredoxin reductase to transfer electrons . Fd bisd , P450 bisd , ferredoxin reductase, and NADH are involved in the BPA degradation by S. bisphenolicum AO1 (Figure ).…”

Section: Discussionmentioning

confidence: 99%

“…Compared with S. bisphenolicum AO1 and E. coli , Shewanella oneidensis has excellent EET properties and promising value in pollutant removal and energy generation applications such as microbial fuel cells and microbial electrolysis in water treatment. , Moreover, the ability to reduce and absorb heavy metals makes S. oneidensis a candidate for use in wastewater treatment. − Biofilm formation by S. oneidensis can enhance its performance in biochemical water treatment, because engineered biofilms can be applied to membrane bioreactors for efficient pollutant degradation and to microbial fuel cells for improved electricity generation. , Curli biogenesis (Csg clusters) is strongly associated with S. oneidensis biofilm formation . CsgD, a master regulator of biofilm formation, controls curli and cellulose production .…”

Section: Introductionmentioning

confidence: 99%

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Establishing Efficient Bisphenol A Degradation by Engineering Shewanella oneidensis

Zhou

Hong

2021

Ind. Eng. Chem. Res.

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Bisphenol A (BPA) has been widely used as a plasticizer in the production of synthetic polymers, such as those used in food storage containers and bottles. However, BPA interferes with endocrine systems, causing carcinogenicity, immunotoxicity, and embryotoxicity. Biological water treatment processes scarcely remove BPA, owing to the poor BPA degradation ability and efficiency of the applied microorganisms. Shewanella oneidensis has been studied and used for the biodegradation process in wastewater treatment because of its excellent extracellular electron transfer properties. In this work, we engineered S. oneidensis MR1 to enable BPA degradation by producing ferredoxin (Fdbisd) and cytochrome P450 (P450bisd) originating from Sphingomonas bisphenolicum AO1. The engineered S. oneidensis exhibited a higher BPA degradation efficiency than Escherichia coli producing the same enzymes. The endogenous ferredoxin and ferredoxin reductase of S. oneidensis participated in BPA degradation, and overexpression of mtrC, omcA, and So0521, which encode S. oneidensis cytochromes, decreased BPA. We developed BPA-degrading S. oneidensis biofilms. This study provides insights into biocatalyst utilization for the biological degradation of toxic organic compounds.

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Establishing Efficient Bisphenol A Degradation by Engineering Shewanella oneidensis

Zhou

Hong

2021

Ind. Eng. Chem. Res.

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“…There are many cell types, although in general the cell is connected to an external circuit; the composition of the cell consists of an anodic and cathodic chamber separated by a proton exchange membrane. The anodic chamber is customarily in anaerobiosis, and the cathodic chamber is in contact with oxygen [13][14][15]. In this sense, Cecconet et al (2018) used agroindustrial waste in their cells, which were made with graphite electrodes, and managed to generate voltage peaks of 637 mV with an external resistance of 20.4 Ω on day 51.…”

Section: Introductionmentioning

confidence: 99%

Use of Onion Waste as Fuel for the Generation of Bioelectricity

et al. 2022

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The enormous environmental problems that arise from organic waste have increased due to the significant population increase worldwide. Microbial fuel cells provide a novel solution for the use of waste as fuel for electricity generation. In this investigation, onion waste was used, and managed to generate maximum peaks of 4.459 ± 0.0608 mA and 0.991 ± 0.02 V of current and voltage, respectively. The conductivity values increased rapidly to 179,987 ± 2859 mS/cm, while the optimal pH in which the most significant current was generated was 6968 ± 0.286, and the ° Brix values decreased rapidly due to the degradation of organic matter. The microbial fuel cells showed a low internal resistance (154,389 ± 5228 Ω), with a power density of 595.69 ± 15.05 mW/cm2 at a current density of 6.02 A/cm2; these values are higher than those reported by other authors in the literature. The diffractogram spectra of the onion debris from FTIR show a decrease in the most intense peaks, compared to the initial ones with the final ones. It was possible to identify the species Pseudomona eruginosa, Acinetobacter bereziniae, Stenotrophomonas maltophilia, and Yarrowia lipolytica adhered to the anode electrode at the end of the monitoring using the molecular technique.

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