Engineering resistance against geminiviruses: A review of suppressed natural defenses and the use of RNAi and the CRISPR/Cas system

Loriato, Virgílio A. P.; Martins, Laura Gonçalves Costa; Euclydes, Nívea C.; Reis, Pedro A. B.; Duarte, Christiane Eliza Motta; Fontes, Elizabeth P. B.

doi:10.1016/j.plantsci.2020.110410

Cited by 26 publications

(20 citation statements)

References 103 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This has sparked a push to develop treatments and vaccines 55 . Present antiviral strategies, both viral protein interfering and gene silencing approaches, are either only minimally effective or are eventually subverted by conferred resistance from a rapidly evolving viral genome 51,[56][57][58][59][60] . However, development of antivirals specifically targeting the ssDNA binding of Reps could more effectively retain long-term resistance.…”

Section: Discussionmentioning

confidence: 99%

Molecular underpinnings of ssDNA specificity by Rep HUH-endonucleases and implications for HUH-tag multiplexing and engineering

Tompkins

Houtti

Litzau

et al. 2020

Preprint

View full text Add to dashboard Cite

Replication initiator proteins (Reps) from the HUH-endonuclease superfamily process specific single-stranded DNA (ssDNA) sequences to initiate rolling circle/hairpin replication in viruses, such as crop ravaging geminiviruses and human disease causing parvoviruses. In biotechnology contexts, Reps are the basis for HUH-tag bioconjugation and a critical adeno-associated virus genome integration tool. We solved the first co-crystal structures of Reps complexed to ssDNA, revealing a key motif for conferring sequence specificity and anchoring a bent DNA architecture. In combination, we developed a deep sequencing cleavage assay termed HUH-seq to interrogate subtleties in Rep specificity, and demonstrate how differences can be exploited for multiplexed HUH-tagging. Together, our insights allowed us to engineer a Rep chimera to predictably alter sequence specificity. These results have important implications for modulating viral infections, developing Rep-based genomic integration tools, and enabling massively parallel HUH-tag barcoding and bioconjugation applications.

show abstract

Section: Discussionmentioning

confidence: 99%

Molecular underpinnings of ssDNA specificity by Rep HUH-endonucleases and implications for HUH-tag multiplexing and engineering

Tompkins

Houtti

Litzau

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…They can interfere with both PTGS and TGS in all three steps of the processes (Figure 2). They also act downstream of TGS, directly or indirectly affecting DNA methylation [13]. Mechanistically, geminiviral VSRs either suppress the activity or repress the accumulation (expression) of RNA-silencing machinery components.…”

Section: Geminiviral Suppressors Of Ptgs and Tgsmentioning

confidence: 99%

“…Like any other plant virus, geminiviruses both activate and suppress RNA-silencingmediated antiviral immunity. Infected hosts accumulate geminivirus-derived small interfering RNA (siRNA) of 21-24 bp, and almost all geminivirus proteins have been shown to suppress critical steps in the RNA-silencing mechanism [13]. Evidence that viral PTI functions against geminiviruses includes the finding that PTI upstream receptors are virulence targets of geminivirus proteins, which can also suppress downstream PTI-like responses, fulfilling the concept that PTI must be inhibited for successful infection [14,15].…”

Section: Introductionmentioning

confidence: 99%

Geminiviral Triggers and Suppressors of Plant Antiviral Immunity

et al. 2021

View full text Add to dashboard Cite

Geminiviruses are circular single-stranded DNA plant viruses encapsidated into geminate virion particles, which infect many crops and vegetables and, hence, represent significant agricultural constraints worldwide. To maintain their broad-range host spectrum and establish productive infection, the geminiviruses must circumvent a potent plant antiviral immune system, which consists of a multilayered perception system represented by RNA interference sensors and effectors, pattern recognition receptors (PRR), and resistance (R) proteins. This recognition system leads to the activation of conserved defense responses that protect plants against different co-existing viral and nonviral pathogens in nature. Furthermore, a specific antiviral cell surface receptor signaling is activated at the onset of geminivirus infection to suppress global translation. This review highlighted these layers of virus perception and host defenses and the mechanisms developed by geminiviruses to overcome the plant antiviral immunity mechanisms.

show abstract

“…Figure 1 puts these mechanisms in context with geminiviral life cycle. For a more detailed look at these approaches, see Loriato et al (2020).…”

Section: Engineered Resistancementioning

confidence: 99%

Geminivirus Resistance: A Minireview

Beam

Ascencio-Ibáñez

2020

Front. Plant Sci.

View full text Add to dashboard Cite

A continuing challenge to crop production worldwide is the spectrum of diseases caused by geminiviruses, a large family of small circular single-stranded DNA viruses. These viruses are quite diverse, some containing mono-or bi-partite genomes, and infecting a multitude of monocot and dicot plants. There are currently many efforts directed at controlling these diseases. While some of the methods include controlling the insect vector using pesticides or genetic insect resistance (Rodrí guez-Ló pez et al., 2011), this review will focus on the generation of plants that are resistant to geminiviruses themselves. Genetic resistance was traditionally found by surveying the wild relatives of modern crops for resistance loci; this method is still widely used and successful. However, the quick rate of virus evolution demands a rapid turnover of resistance genes. With better information about virus-host interactions, scientists are now able to target early stages of geminivirus infection in the host, preventing symptom development and viral DNA accumulation.

show abstract

Engineering resistance against geminiviruses: A review of suppressed natural defenses and the use of RNAi and the CRISPR/Cas system

Cited by 26 publications

References 103 publications

Molecular underpinnings of ssDNA specificity by Rep HUH-endonucleases and implications for HUH-tag multiplexing and engineering

Molecular underpinnings of ssDNA specificity by Rep HUH-endonucleases and implications for HUH-tag multiplexing and engineering

Geminiviral Triggers and Suppressors of Plant Antiviral Immunity

Geminivirus Resistance: A Minireview

Contact Info

Product

Resources

About