Engineering protein modules for diagnostic applications

Blackstock, Daniel; Park, Miso; Sun, Qing; Tsai, Shen‐Long; Chen, Wilfred

doi:10.1016/j.coche.2013.08.001

Cited by 3 publications

(2 citation statements)

References 58 publications

(46 reference statements)

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“…Consequently, there exists a continuous growing interest in applications of process system engineering techniques to biomedical problems 3 . In particular, there is a great interest in the analysis of the protein in relation to biological events, such as ligand binding, enzymatic modification and protease cleavage 4 .…”

Section: Introductionmentioning

confidence: 99%

Molecular refrigerators: a new approach in anti-cancer therapy

Lucia¹

2013

OA Medical Hypothesis

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Introduction The temperature difference between normal and cancer cells was proven to be one of the fundamental quantities in the cancer growth. The allosteric transition is a conformational change of a protein conditioned to bind at a specific site; in an enzyme, two different parts can oscillate around their equilibrium state; if the temperature increases, these parts increase their oscillating amplitudes, decreasing in their functionality. This paper discusses a new method for anti-cancer therapy. Hypothesis The entropy generation approach has been used in order to evaluate the stationary conditions of tumoural cells in relation to the transport processes. The tumoural systems can assume all the values of volume, temperature, chemical reaction rate and the time of chemical reaction inside its stationary range of variation; outside of this range, the cancer cannot develop and cell dies. Evaluation of Hypothesis The control over these physicalchemical processes can be obtained by using molecular refrigerators, which help control the temperature of the cells. The aim is to maintain the temperature of a normal cell and not to allow the cell to increase its temperature up to the cancer state. This brings about conformation changes in a protein by supplying free energy on one site of the molecule, required for driving the cooling process. This thermalisation is suggested as anti-cancer therapy. The temperature variation is evaluated by using the entropy generation approach. Conclusion The results obtained represent a new approach to the study of the cancer and for the development of new anticancer therapy based on molecular refrigeration.

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Section: Introductionmentioning

confidence: 99%

Molecular refrigerators: a new approach in anti-cancer therapy

Lucia¹

2013

OA Medical Hypothesis

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“…Furthermore, IgG antibodies persisted in O. viverrini -infected hosts after antihelmintic treatment [ 23 ], and patients with a light infection excreting fewer than 50 eggs/g feces, which were undetectable by FECT, showed positive reactivity with the recombinant protein [ 24 ]. The detection of IgG4 antibodies [ 25 ] or the engineering of recombinant protein targets using rational design and directed evolution can improve the affinity and specificity of protein target binding to specific antibodies, thereby increasing the sensitivity and specificity of diagnosis [ 26 , 27 ]. Moreover, other immunoglobulin isotypes or specific antibodies should be detected in alternative samples, such as saliva and urine.…”

Section: Discussionmentioning

confidence: 99%

Multi-epitope protein production and its application in the diagnosis of opisthorchiasis

Sripa,

Chaiwong

2024

Parasites Vectors

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Background Opisthorchiasis and cholangiocarcinoma (CCA) continue to be public health concerns in many Southeast Asian countries. Although the prevalence of opisthorchiasis is declining, reported cases tend to have a light-intensity infection. Therefore, early detection by using sensitive methods is necessary. Several sensitive methods have been developed to detect opisthorchiasis. The immunological detection of antigenic proteins has been proposed as a sensitive method for examining opisthorchiasis. Methods The Opisthorchis viverrini antigenic proteins, including cathepsin B (OvCB), asparaginyl endopeptidase (OvAEP), and cathepsin F (OvCF), were used to construct multi-antigenic proteins. The protein sequences of OvCB, OvAEP, and OvCF, with a high probability of B cell epitopes, were selected using BepiPred 1.0 and the IEDB Analysis Resource. These protein fragments were combined to form OvCB_OvAEP_OvCF recombinant DNA, which was then used to produce a recombinant protein in Escherichia coli strain BL21(DE3). The potency of the recombinant protein as a diagnostic target for opisthorchiasis was assessed using immunoblotting and compared with that of the gold standard method, the modified formalin-ether concentration technique. Results The recombinant OvCB_OvAEP_OvCF protein showed strong reactivity with total immunoglobulin G (IgG) antibodies against light-intensity O. viverrini infections in the endemic areas. Consequently, a high sensitivity (100%) for diagnosing opisthorchiasis was reported. However, cross-reactivity with sera from other helminth and protozoan infections (including taeniasis, strongyloidiasis, giardiasis, E. coli infection, enterobiasis, and mixed infection of Echinostome spp. and Taenia spp.) and no reactivity with sera from patients with non-parasitic infections led to a reduced specificity of 78.4%. In addition, the false negative rate (FNR), false positive rate (FPR), positive predictive value (PPV), negative predictive value (NPV), and diagnostic accuracy were 0%, 21.6%, 81.4%, 100%, and 88.9%, respectively. Conclusions The high sensitivity of the recombinant OvCB_OvAEP_OvCF protein in detecting opisthorchiasis demonstrates its potential as an opisthorchiasis screening target. Nonetheless, research on reducing cross-reactivity should be undertaken by detecting other antibodies in other sample types, such as saliva, urine, and feces. Graphical Abstract

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Nanoscale materials and approaches for optical glucose assays

Xianyu

Jiang

2014

Current Opinion in Chemical Engineering

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Engineering protein modules for diagnostic applications

Cited by 3 publications

References 58 publications

Molecular refrigerators: a new approach in anti-cancer therapy

Molecular refrigerators: a new approach in anti-cancer therapy

Multi-epitope protein production and its application in the diagnosis of opisthorchiasis

Nanoscale materials and approaches for optical glucose assays

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