Engineering plant family TPS into cyanobacterial host for terpenoids production

Rautela, Akhil; Kumar, Sanjay

doi:10.1007/s00299-022-02892-9

Cited by 16 publications

(19 citation statements)

References 106 publications

(153 reference statements)

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“…Moreover, cyanobacteria are viewed as promising cell factories for the production of chemicals (biofuels, biodegradable bioplastics, drugs, solvents, etc.) from highly abundant natural resources: solar energy, water (fresh/marine), CO 2 and minerals [ 74 , 75 , 81 ], thanks to their active and robust photoautotrophic metabolism [ 55 , 56 ] and the synthetic biology tools of model species [ 82 ], as shown in Figure 2 .…”

Section: Biological Importance and Biotechnological Interests Of Cyan...mentioning

confidence: 99%

The Glutathione System: A Journey from Cyanobacteria to Higher Eukaryotes

et al. 2023

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From bacteria to plants and humans, the glutathione system plays a pleiotropic role in cell defense against metabolic, oxidative and metal stresses. Glutathione (GSH), the γ-L-glutamyl-L-cysteinyl-glycine nucleophile tri-peptide, is the central player of this system that acts in redox homeostasis, detoxification and iron metabolism in most living organisms. GSH directly scavenges diverse reactive oxygen species (ROS), such as singlet oxygen, superoxide anion, hydrogen peroxide, hydroxyl radical, nitric oxide and carbon radicals. It also serves as a cofactor for various enzymes, such as glutaredoxins (Grxs), glutathione peroxidases (Gpxs), glutathione reductase (GR) and glutathione-S-transferases (GSTs), which play crucial roles in cell detoxication. This review summarizes what is known concerning the GSH-system (GSH, GSH-derived metabolites and GSH-dependent enzymes) in selected model organisms (Escherichia coli, Saccharomyces cerevisiae, Arabidopsis thaliana and human), emphasizing cyanobacteria for the following reasons. Cyanobacteria are environmentally crucial and biotechnologically important organisms that are regarded as having evolved photosynthesis and the GSH system to protect themselves against the ROS produced by their active photoautotrophic metabolism. Furthermore, cyanobacteria synthesize the GSH-derived metabolites, ergothioneine and phytochelatin, that play crucial roles in cell detoxication in humans and plants, respectively. Cyanobacteria also synthesize the thiol-less GSH homologs ophthalmate and norophthalmate that serve as biomarkers of various diseases in humans. Hence, cyanobacteria are well-suited to thoroughly analyze the role/specificity/redundancy of the players of the GSH-system using a genetic approach (deletion/overproduction) that is hardly feasible with other model organisms (E. coli and S. cerevisiae do not synthesize ergothioneine, while plants and humans acquire it from their soil and their diet, respectively).

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Section: Biological Importance and Biotechnological Interests Of Cyan...mentioning

confidence: 99%

The Glutathione System: A Journey from Cyanobacteria to Higher Eukaryotes

et al. 2023

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“…Terpenes constitute a large family of chemicals often produced by plants (attraction of pollinators and/or protection against pathogens) in low quantities and at high prices. They are used for the production of fragrances, pharmaceuticals, pesticides, solvents and potentially biofuels [1][2][3]. All terpenes (general formula (C 5 H 8 ) n ) derive from the five-carbon (C5) building blocks dimethylallyl pyrophosphate (DMAPP) and isopentenyl pyrophosphate (IPP).…”

Section: Introductionmentioning

confidence: 99%

Impact of Carbon Fixation, Distribution and Storage on the Production of Farnesene and Limonene in Synechocystis PCC 6803 and Synechococcus PCC 7002

Vincent,

Blanc-Garin,

Chenebault

et al. 2024

IJMS

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Terpenes are high-value chemicals which can be produced by engineered cyanobacteria from sustainable resources, solar energy, water and CO2. We previously reported that the euryhaline unicellular cyanobacteria Synechocystis sp. PCC 6803 (S.6803) and Synechococcus sp. PCC 7002 (S.7002) produce farnesene and limonene, respectively, more efficiently than other terpenes. In the present study, we attempted to enhance farnesene production in S.6803 and limonene production in S.7002. Practically, we tested the influence of key cyanobacterial enzymes acting in carbon fixation (RubisCO, PRK, CcmK3 and CcmK4), utilization (CrtE, CrtR and CruF) and storage (PhaA and PhaB) on terpene production in S.6803, and we compared some of the findings with the data obtained in S.7002. We report that the overproduction of RubisCO from S.7002 and PRK from Cyanothece sp. PCC 7425 increased farnesene production in S.6803, but not limonene production in S.7002. The overexpression of the crtE genes (synthesis of terpene precursors) from S.6803 or S.7002 did not increase farnesene production in S.6803. In contrast, the overexpression of the crtE gene from S.6803, but not S.7002, increased farnesene production in S.7002, emphasizing the physiological difference between these two model cyanobacteria. Furthermore, the deletion of the crtR and cruF genes (carotenoid synthesis) and phaAB genes (carbon storage) did not increase the production of farnesene in S.6803. Finally, as a containment strategy of genetically modified strains of S.6803, we report that the deletion of the ccmK3K4 genes (carboxysome for CO2 fixation) did not affect the production of limonene, but decreased the production of farnesene in S.6803.

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“…Terpenes are a large family of chemicals with the formula (C 5 H 8 ) n that are often produced by plants where they act in their photoautotrophic metabolism, protection against pathogens, and/or attraction of pollinators. Many high-value terpenes are exploited by cosmetic, food, pharmaceutical, and/or biofuel industries, including bisabolene, farnesene, limonene, and pinene, the focus of the present study [ 1 , 2 ].…”

Section: Introductionmentioning

confidence: 99%

“…Cyanobacteria possess the methylerythritol-4-phosphate (MEP) pathway that produces GPP and FPP ( Figure 1 ), which can be transformed into terpenes. For this purpose, synthetic genes encoding terpene synthases need to be adapted to the cyanobacterial codon usage and subsequently introduced and expressed in cyanobacteria [ 1 , 2 ].…”

Section: Introductionmentioning

confidence: 99%

Exploring the Potential of the Model Cyanobacteria Synechococcus PCC 7002 and PCC 7942 for the Photoproduction of High-Value Terpenes: A Comparison with Synechocystis PCC 6803

et al. 2023

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We have performed the first comparative analysis of the potential of two physiologically-diverse model cyanobacteria, Synechococcus PCC 7002 (S.7002) and Synechococcus elongatus PCC 7942 (S.7942), for the photosynthetic production of four chemically-different high-value terpenes: two monoterpenes limonene and pinene, and two sesquiterpenes bisabolene and farnesene. We showed, for the first time, that S.7002 and S.7942 can produce farnesene and bisabolene, respectively. Both cyanobacteria produced farnesene (S.7942 produced more efficiently than S.7002) more efficiently than the other tested terpenes (especially pinene, the weakest produced terpene). S.7002 produced limonene more efficiently than bisabolene, whereas S.7942 produced bisabolene more efficiently than limonene. These findings suggest that S.7942 is better suited to produce sesquiterpenes than monoterpenes. Interestingly, higher levels of terpenes were produced by S.7942 and S.7002 expressing a terpene-synthase gene from both an RSF1010-derived replicating plasmid and a neutral chromosomal site, as compared to either the plasmid alone or the chromosome alone. These results suggest that in both cyanobacteria, the production of terpenes is more limited by the activity of terpene synthases than the abundance of terpene precursors. Finally, higher levels of terpenes were produced by S.7002 growing on urea (a frequent pollutant) as compared to nitrate or ammonium, the standard nitrogen sources for cyanobacteria.

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Engineering plant family TPS into cyanobacterial host for terpenoids production

Cited by 16 publications

References 106 publications

The Glutathione System: A Journey from Cyanobacteria to Higher Eukaryotes

The Glutathione System: A Journey from Cyanobacteria to Higher Eukaryotes

Impact of Carbon Fixation, Distribution and Storage on the Production of Farnesene and Limonene in Synechocystis PCC 6803 and Synechococcus PCC 7002

Exploring the Potential of the Model Cyanobacteria Synechococcus PCC 7002 and PCC 7942 for the Photoproduction of High-Value Terpenes: A Comparison with Synechocystis PCC 6803

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