Engineering, on‐demand manufacturing, and scaling‐up of polymeric nanocapsules

Abstract

Polymeric nanocapsules are versatile delivery systems with the capacity to load lipophilic drugs in their oily nucleus and hydrophilic drugs in their polymeric shell. The objective of this work was to expand the technological possibilities to prepare customized nanocapsules. First, we adapted the solvent displacement technique to modulate the particle size of the resulting nanocapsules in the 50–500 nm range. We also produced nanosystems with a shell made of one or multiple polymer layers i.e. chitosan, dextran sulphate, hyaluronate, chondroitin sulphate, and alginate. In addition, we identified the conditions to translate the process into a miniaturized high‐throughput tailor‐made fabrication that enables massive screening of formulations. Finally, the production of the nanocapsules was scaled‐up both in a batch production, and also using microfluidics. The versatility of the properties of these nanocapsules and their fabrication technologies is expected to propel their advance from bench to clinic.

Nanocarrier‐Mediated Cytosolic Delivery of Biopharmaceuticals

“…Drug delivery using nanocarriers is a promising strategy to improve therapeutic efficacy. [ 6 ] So far, over 65 nanomedicines in the form of liposomes, [ 7 ] nanocrystals, [ 8 ] inorganic nanoparticles, [ 7 ] polymeric nanoparticles, [ 9 ] nanocapsules, [ 10 ] and protein nanoparticles [ 7 ] are approved for clinical use ( Table 2 and Figure ). Various approaches have been used to engineer biopharmaceuticals into micro‐ or nanoscale structures to enhance their therapeutic potency, including physical fabrication, [ 11 ] covalent molecular modification, [ 12 ] encapsulation by biodegradable hydrogels based on natural and synthetic polymers, [ 13 ] and nanostructuring processes.…”

Hyaluronic Acid Nanocapsules as a Platform for Needle-Free Vaccination

“…Hyaluronic acid (HA) was chosen as a corona for various reasons: its biosafety, biocompatibility, and because of its use as a material in biomedical applications [26]; its low molecular weight fragmentation products could activate immune cells through activation or regulation of Toll-like receptors 4 and 2 and by stimulating the secretion of different cytokines [27,28]; it is a product that has been frequently used in the cosmetic and nutricosmetic industry [29]. The use of polymeric nanocapsules as a vehicle in transdermal antigen delivery is due to their structure (nucleus-corona) which allows the incorporation of oils and hydrophobic molecules with immunoadjuvant properties in the nucleus and of hydrophilic active molecules (such as an antigen or a drug) that are embedded in the polymeric shell [30].…”

Design of Polymeric Nanocapsules for Intranasal Vaccination against Mycobacterium Tuberculosis: Influence of the Polymeric Shell and Antigen Positioning

“…The NCs were synthetized by the solvent-displacement method, as described previously [16,17]. For CS NCs, the organic phase was prepared with 125 µL of a mixture of linoleic acid an Miglyol ® 812 (9.5:3, v/v ratio) and 1 mg of IMQ, 20 mg of the PEGylated phosphoethanolamine 18:0 PE-PEG1000 and 25 µL of an aqueous solution of 200 mg/mL sodium cholate in 5 mL of ethanol.…”