Engineering of the mRNA-interfering complementary RNA immune system against viral infection.

Hirashima, Akikazu; Sawaki, Saeko; Inokuchi, Yoshio; Inouye, Masayori

doi:10.1073/pnas.83.20.7726

Cited by 32 publications

(14 citation statements)

References 15 publications

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“…It has been shown that replication ofthe RNA coliphage SP can be inhibited by antisense RNA by expressing various antisense viral sequences in the host (9,10). The antisense RNA for the maturase gene was the most effective molecule in these replication inhibition assays, and antisense RNA complementary to sequences at the 3' end of the viral RNA had a lesser effect.…”

mentioning

confidence: 95%

Protection against tobacco mosaic virus in transgenic plants that express tobacco mosaic virus antisense RNA.

Powell

Stark

Sanders

et al. 1989

Proc. Natl. Acad. Sci. U.S.A.

111

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Transgenic tobacco plants that express RNA sequences complementary to the tobacco mosaic virus (TMV) coat protein (CP) coding sequence with or without the tRNAlike structure at the 3' end of the TMV RNA were produced. Progeny of self-pollinated plants were challenged with TMV to determine their resistance to infection. Plants that expressed RNA sequences complementary to the CP coding region and the 3' untranslated region, including the tRNA-like sequences, were protected from infection by TMV at low levels of inocu- (6) and the expression of the insertion element ISJO (7). In these examples the antisense RNA presumably anneals with its corresponding mRNA and prevents translation (7,8).It has been shown that replication ofthe RNA coliphage SP can be inhibited by antisense RNA by expressing various antisense viral sequences in the host (9, 10). The antisense RNA for the maturase gene was the most effective molecule in these replication inhibition assays, and antisense RNA complementary to sequences at the 3' end of the viral RNA had a lesser effect. Since the latter region contained only the 3' one-third ofthe replicase gene and the 3' noncoding region, the result indicates that antisense RNA may be effective at blocking replication in RNA viruses independent of its ability to block translation. (Fig. 1B)

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mentioning

confidence: 95%

Protection against tobacco mosaic virus in transgenic plants that express tobacco mosaic virus antisense RNA.

Powell

Stark

Sanders

et al. 1989

Proc. Natl. Acad. Sci. U.S.A.

111

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“…Furthermore, RNA phages paved the way for antisense-based gene therapy via the generation of the so-called ‘mRNA-interfering complementary RNA (micRNA) immune system' for the prevention of phage SP proliferation [39,40]. …”

Section: Family Of Rna Phagesmentioning

confidence: 99%

The True Story and Advantages of RNA Phage Capsids as Nanotools

et al. 2016

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RNA phages are often used as prototypes for modern recombinant virus-like particle (VLP) technologies. Icosahedral RNA phage VLPs can be formed from coat proteins (CPs) and are efficiently produced in bacteria and yeast. Both genetic fusion and chemical coupling have been successfully used for the production of numerous chimeras based on RNA phage VLPs. In this review, we describe advances in RNA phage VLP technology along with the history of the Leviviridae family, including its taxonomical organization, genomic structure, and important role in the development of molecular biology. Comparative 3D structures of different RNA phage VLPs are used to explain the level of VLP tolerance to foreign elements displayed on VLP surfaces. We also summarize data that demonstrate the ability of CPs to tolerate different organic (peptides, oligonucleotides, and carbohydrates) and inorganic (metal ions) compounds either chemically coupled or noncovalently added to the outer and/or inner surfaces of VLPs. Finally, we present lists of nanotechnological RNA phage VLP applications, such as experimental vaccines constructed by genetic fusion and chemical coupling methodologies, nanocontainers for targeted drug delivery, and bioimaging tools.

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“…Antisense approaches have been used previously in this system (38), but we believed more efficient ablation would be achieved using a ribozyme approach with catalytic efficiency. Considering that targeting the initiating AUG codon or adjacent sequences may increase the antisense effect of ribozymes (39,40), ribozyme-6 was chosen for transfection and expression in ROS 17/2.8 cells. Because the alternative splice that occurs in the osteoblast ␣ 1C mRNA is downstream in the coding region at transmembrane segments IVS3-IVS4 (11), ribozyme-6 was expected to cleave transcripts encoding both of these isoforms.…”

Section: Figmentioning

confidence: 99%

Ribozyme Ablation Demonstrates That the Cardiac Subtype of the Voltage-sensitive Calcium Channel Is the Molecular Transducer of 1,25-Dihydroxyvitamin D3-stimulated Calcium Influx in Osteoblastic Cells

Liu¹,

Li²,

Karin³

et al. 2000

Journal of Biological Chemistry

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Engineering of the mRNA-interfering complementary RNA immune system against viral infection.

Cited by 32 publications

References 15 publications

Protection against tobacco mosaic virus in transgenic plants that express tobacco mosaic virus antisense RNA.

Protection against tobacco mosaic virus in transgenic plants that express tobacco mosaic virus antisense RNA.

The True Story and Advantages of RNA Phage Capsids as Nanotools

Ribozyme Ablation Demonstrates That the Cardiac Subtype of the Voltage-sensitive Calcium Channel Is the Molecular Transducer of 1,25-Dihydroxyvitamin D3-stimulated Calcium Influx in Osteoblastic Cells

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