Engineering of Strain Producing C-Terminal Fragment of Clostridium Perfringens Alpha-Toxin

Abstract

An Esсherichia coli 42cpa-c strain synthesizing C-terminal fragment of Clostridium perfringens alpha-toxin was engineered. The strain is distinguished by productive capacity 1.2 mg of target protein per 1 L of cultural liquid. The protein contains octahistidine peptide at C-end of the molecule, enabling to carry out its one-stage purification by metal-affine chromatography on Ni2+-NTA resin. The resulting CPA-C protein preparation is potentially suitable for formulation of the derived vaccine.