Engineering of Saccharomyces cerevisiae as a consolidated bioprocessing host to produce cellulosic ethanol: Recent advancements and current challenges

Sharma, Juhi; Kumar, Vinod; Prasad, Rajendra; Gaur, Naseem A.

doi:10.1016/j.biotechadv.2022.107925

Cited by 54 publications

(32 citation statements)

References 150 publications

(137 reference statements)

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“…LCB is the most abundant raw material on the earth, with an annual output of about 200 billion tons. It is often composed of various plant residues, most of which are agricultural wastes, accumulating in the form of LCB every year ( Sharma et al, 2022 ). Among them, cellulose is directly converted to resources for degradation, saccharification and other processing.…”

Section: Resultsmentioning

confidence: 99%

Screening of cellulose-degrading yeast and evaluation of its potential for degradation of coconut oil cake

Liu

Zhong

et al. 2022

Front. Microbiol.

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Coconut oil cake (COC), a byproduct of oil extraction, contains high levels of cellulose. The aim of this study was to isolate a cellulose-degrading yeast from rotten dahlia that can effectively use COC as the only carbon source for cellulase secretion. Based on screening, Meyerozyma guillermondii CBS 2030 (M. guillermondii) was identified as a potential candidate, with the highest cellulolytic activity among the yeast strains isolated, with the carboxymethyl cellulase (CMCase) activity reaching 102.96 U/mL on day 5. The cellulose in COC samples was evaluated before and after degradation by M. guillermondii. Analysis based on field emission scanning electron microscopy (FESEM) revealed that the COC structure was changed significantly during the treatment, indicating effective hydrolysis. Fourier transform infrared spectroscopy (FTIR) of the modified functional groups indicated successful depolymerization of coconut cake. X-ray diffraction (XRD) and analysis of color differences established effective degradation of COC by M. guillermondii. The results demonstrate that M. guillermondii effectively secretes CMCase and degrades cellulose, which has important practical significance in COC degradation.

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Section: Resultsmentioning

confidence: 99%

Screening of cellulose-degrading yeast and evaluation of its potential for degradation of coconut oil cake

Liu

Zhong

et al. 2022

Front. Microbiol.

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“…Although the quantification of the type of phenolics was not conducted in this study, it is imperative to assess the TPCs formation probability, i.e., their presence in the hydrolysates, as this influences fermentation outcomes, especially when using the popular ethanol-producing strain Saccharomyces cerevisiae (S. cerevisiae). Although different classes of by-products might be present in the hydrolysates of pretreated agro-waste, TPCs were the focus of this study, for which 4-Hydroxy-3-methoxycinnamaldehyde, coniferyl alcohol, and p-coumaric acid are prevalent, among others [44]. It was determined that 4-Hydroxy-3-methoxycinnamaldehyde (coniferyl aldehyde) inhibited S. cerevisiae at 0.32 g/L [45], a very low threshold.…”

Section: Analysis Of Reducible Sugar Production and Residual Phenolic...mentioning

confidence: 99%

Nepenthes mirabilis Fractionated Pitcher Fluid Use for Mixed Agro-Waste Pretreatment: Advocacy for Non-Chemical Use in Biorefineries

et al. 2022

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This study determined whether it is feasible to pretreat mixed agro-waste of different particle sizes using the pitcher fluid of Nepenthes mirabilis (N. mirabilis), which is known to digest leaf litter due to the enzyme cocktail contained in the fluid. This is due to the need for the holocellulolysis (a source of fermentable sugars) of mixed agro-waste to produce fermentable hydrolysates. The pitcher fluid was fractionated (<3 kDa, ˃3 kDa, <10 kDa, ˃10 kDa) and slurrified with the mixed agro-waste, i.e., 25% (w/w) for each waste—orange peels, apple peels, maize cobs, grape pomace, and oak plant leaf litter of various particle sizes, i.e., >75 µm x < 106 µm and >106 µm. The process of producing a high concentration of total reducible sugars (TRSs) with the lowest production of total phenolic compounds (TPCs) was determined to be a particle size of >106 µm, pretreatment for 72 h, and an enzyme fraction of <10 kDa, whereby 97 g/L of TRSs were produced with a significantly lower TPCs load (1 g/L). Furthermore, the <10 kDa showed preferable physico-chemical properties, with the highest reduction-oxidation potential including acidity. Several enzymes, i.e., β-1,3-Glucanase, Putative peroxidase 27, Thaumatin-like protein, among others, were identified in the <10 kDa fraction, i.e., enzymes known to perform various functions in plant-based waste. Therefore, there is a need for the renewable energy industry to consider solely using pitcher fluids to pretreat mixed agro-waste for fermentable hydrolysates’ production, which can be used as liquid feedstock for the bioenergy and/or biorefinery industries for environmental pollution reduction.

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“…Researchers cultured this yeast to learn more about the biology of the eukaryotic cell and, ultimately, human biology [ 15 ]. Yeasts have recently been used to produce ethanol for the biofuel industry and to generate electricity in microbial fuel cells [ 13 , 16 , 17 ].…”

Section: Yeasts and Their Genomic Sequencesmentioning

confidence: 99%

“…The complex sugars are then broken down into simple sugars by glucoamylase. Because S. cerevisiae spontaneously produces ethanol by converting one mole of glucose (180 g) into two moles of ethanol (92 g) plus two moles of carbon-dioxide (88 g) and energy (26.4 Kcal), yeast is added to convert the sugars to ethanol, which is then distilled to produce ethanol with up to 96 percent purity [ 16 , 40 ] Saccharomyces spp. have been genetically engineered to ferment xylose, a major fermentable sugar found in cellulosic biomasses like agricultural residues, paper waste, and wood chips [ 41 , 42 ].…”

Section: Biofuel Productionmentioning

confidence: 99%

Yeast Genomics and Its Applications in Biotechnological Processes: What Is Our Present and Near Future?

Tullio

2022

JoF

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Since molecular biology and advanced genetic techniques have become important tools in a variety of fields of interest, including taxonomy, identification, classification, possible production of substances and proteins, applications in pharmacology, medicine, and the food industry, there has been significant progress in studying the yeast genome and its potential applications. Because of this potential, as well as their manageability, safety, ease of cultivation, and reproduction, yeasts are now being extensively researched in order to evaluate a growing number of natural and sustainable applications to provide many benefits to humans. This review will describe what yeasts are, how they are classified, and attempt to provide a rapid overview of the many current and future applications of yeasts. The review will then discuss how yeasts—including those molecularly modified—are used to produce biofuels, proteins such as insulin, vaccines, probiotics, beverage preparations, and food additives and how yeasts could be used in environmental bioremediation and biocontrol for plant infections. This review does not delve into the issues raised during studies and research, but rather presents the positive outcomes that have enabled several industrial, clinical, and agricultural applications in the past and future, including the most recent on cow-free milk.

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Engineering of Saccharomyces cerevisiae as a consolidated bioprocessing host to produce cellulosic ethanol: Recent advancements and current challenges

Cited by 54 publications

References 150 publications

Screening of cellulose-degrading yeast and evaluation of its potential for degradation of coconut oil cake

Screening of cellulose-degrading yeast and evaluation of its potential for degradation of coconut oil cake

Nepenthes mirabilis Fractionated Pitcher Fluid Use for Mixed Agro-Waste Pretreatment: Advocacy for Non-Chemical Use in Biorefineries

Yeast Genomics and Its Applications in Biotechnological Processes: What Is Our Present and Near Future?

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