Engineering of Implantable Cartilaginous Structures from Bone Marrow–Derived Mesenchymal Stem Cells

Hannouche, Didier; Terai, Hidetomi; Fuchs, James A.; Terada, Shinichi; Zand, Sarvenaz; Nasseri, Boris A.; Petite, Hervé; Sedel, L.; Vacanti, Joseph P.

doi:10.1089/ten.2006.0067

Cited by 68 publications

(24 citation statements)

References 55 publications

(53 reference statements)

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“…of studiesReferencesCell SourceNo. of studies a ReferencesHuman73[48, 50, 52, 53, 168–236]Bone marrow62[48, 50–53, 164, 168, 170–173, 177–180, 182–185, 187, 188, 192, 195–197, 203, 206–210, 212, 216, 217, 219, 221, 223, 227, 230, 232–235, 237–255]Rabbit17[240–242, 246, 249, 252, 255–265]Adipose36[66, 169, 175, 176, 181, 186, 189, 193, 194, 199, 201, 202, 211, 214, 216, 218–220, 224, 228, 229, 231, 235, 242, 256, 257, 260–269]Bovine5[51, 164, 243, 245, 270]Synovium9[174, 191, 200…”

Section: Resultsmentioning

confidence: 99%

“…of studies (%)ReferencesGrowth factorNo. of studies (%)ReferencesTGF-β148 (44%)[50, 169–175, 189, 190, 192, 193, 195, 199, 202, 208, 210, 211, 213, 214, 216, 217, 220, 222–224, 228, 230–232, 234, 235, 244, 246, 249, 252–256, 258, 260–263, 266, 267, 270]SOX-51 (1%)[204]TGF-β332 (29%)[51, 162, 164, 168, 177, 181–184, 197, 200, 205–207, 218, 223–225, 227, 237, 239, 240, 245, 247, 248, 250, 251, 257, 259, 267, 268, 270]SOX-61 (1%)[204]BMP-213 (12%)[188, 202, 213, 219, 225–227, 229, 264, 265, 267, 270, 271]WNT3A1 (1%)…”

Section: Resultsmentioning

confidence: 99%

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The use of mesenchymal stem cells for cartilage repair and regeneration: a systematic review

et al. 2017

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BackgroundThe management of articular cartilage defects presents many clinical challenges due to its avascular, aneural and alymphatic nature. Bone marrow stimulation techniques, such as microfracture, are the most frequently used method in clinical practice however the resulting mixed fibrocartilage tissue which is inferior to native hyaline cartilage. Other methods have shown promise but are far from perfect. There is an unmet need and growing interest in regenerative medicine and tissue engineering to improve the outcome for patients requiring cartilage repair. Many published reviews on cartilage repair only list human clinical trials, underestimating the wealth of basic sciences and animal studies that are precursors to future research. We therefore set out to perform a systematic review of the literature to assess the translation of stem cell therapy to explore what research had been carried out at each of the stages of translation from bench-top (in vitro), animal (pre-clinical) and human studies (clinical) and assemble an evidence-based cascade for the responsible introduction of stem cell therapy for cartilage defects.Main body of abstractThis review was conducted in accordance to PRISMA guidelines using CINHAL, MEDLINE, EMBASE, Scopus and Web of Knowledge databases from 1st January 1900 to 30th June 2015. In total, there were 2880 studies identified of which 252 studies were included for analysis (100 articles for in vitro studies, 111 studies for animal studies; and 31 studies for human studies). There was a huge variance in cell source in pre-clinical studies both of terms of animal used, location of harvest (fat, marrow, blood or synovium) and allogeneicity. The use of scaffolds, growth factors, number of cell passages and number of cells used was hugely heterogeneous.Short conclusionsThis review offers a comprehensive assessment of the evidence behind the translation of basic science to the clinical practice of cartilage repair. It has revealed a lack of connectivity between the in vitro, pre-clinical and human data and a patchwork quilt of synergistic evidence. Drivers for progress in this space are largely driven by patient demand, surgeon inquisition and a regulatory framework that is learning at the same pace as new developments take place.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

The use of mesenchymal stem cells for cartilage repair and regeneration: a systematic review

et al. 2017

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“…Hydrogel–fiber composites are devised with the premise that the incorporation of a ductile fibrous network within a brittle hydrogel matrix increases the stiffness and fracture toughness of the hydrogel, enhancing energy absorption and preventing crack propagation [46, 47]. Fiber-reinforced hydrogels have been investigated in tissue engineering by incorporating a variety of polymeric fibers within hydrogel matrices [48, 49]. Structurally, fiber-reinforced hydrogel provides a discontinuous fibrous structure and proteoglycan matrix in native cartilage.…”

Section: Discussionmentioning

confidence: 99%

Silk microfiber-reinforced silk hydrogel composites for functional cartilage tissue repair

et al. 2015

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Cartilage tissue lacks an intrinsic capacity for self-regeneration due to slow matrix turnover, a limited supply of mature chondrocytes and insufficient vasculature. Although cartilage tissue engineering has achieved some success using agarose as a scaffolding material, major challenges of agarose-based cartilage repair, including non-degradability, poor tissue–scaffold integration and limited processing capability, have prompted the search for an alternative biomaterial. In this study, silk fiber–hydrogel composites (SF–silk hydrogels) made from silk microfibers and silk hydrogels were investigated for their potential use as a support material for engineered cartilage. We demonstrated the use of 100% silk-based fiber–hydrogel composite scaffolds for the development of cartilage constructs with properties comparable to those made with agarose. Cartilage constructs with an equilibrium modulus in the native tissue range were fabricated by mimicking the collagen fiber and proteoglycan composite architecture of native cartilage using biocompatible, biodegradable silk fibroin from Bombyx mori. Excellent chondrocyte response was observed on SF–silk hydrogels, and fiber reinforcement resulted in the development of more mechanically robust constructs after 42 days in culture compared to silk hydrogels alone. Thus, we demonstrate the versatility of silk fibroin as a composite scaffolding material for use in cartilage tissue repair to create functional cartilage constructs that overcome the limitations of agarose biomaterials, and provide a much-needed alternative to the agarose standard.

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“…In order to test this hypothesis, studies should be done to separately determine GAG synthesis and GAG deposition using radiolabelling techniques. It has recently been shown that alginate hydrogels within PGA scaffolds can aid GAG and collagen type II formation, probably due to retention of ECM molecules and chondrogenic growth factors within the construct (Hannouche et al 2007) or the maintenance of the appropriate cell shape, found in native cartilage.…”

Section: Discussionmentioning

confidence: 99%

Effects of chondrogenic and osteogenic regulatory factors on composite constructs grown using human mesenchymal stem cells, silk scaffolds and bioreactors

Augst

Marolt

Freed

et al. 2008

J. R. Soc. Interface.

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Human mesenchymal stem cells (hMSCs) isolated from bone marrow aspirates were cultured on silk scaffolds in rotating bioreactors for three weeks with either chondrogenic or osteogenic medium supplements to engineer cartilage-or bone-like tissue constructs. Osteochondral composites formed from these cartilage and bone constructs were cultured for an additional three weeks in culture medium that was supplemented with chondrogenic factors, supplemented with osteogenic factors or unsupplemented. Progression of cartilage and bone formation and the integration between the two regions were assessed by medical imaging (magnetic resonance imaging and micro-computerized tomography imaging), and by biochemical, histological and mechanical assays. During composite culture (three to six weeks), bone-like tissue formation progressed in all three media to a markedly larger extent than cartilage-like tissue formation. The integration of the constructs was most enhanced in composites cultured in chondrogenic medium. The results suggest that tissue composites with well-mineralized regions and substantially less developed cartilage regions can be generated in vitro by culturing hMSCs on silk scaffolds in bioreactors, that hMSCs have markedly higher capacity for producing engineered bone than engineered cartilage, and that chondrogenic factors play major roles at early stages of bone formation by hMSCs and in the integration of the two tissue constructs into a tissue composite.

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Engineering of Implantable Cartilaginous Structures from Bone Marrow–Derived Mesenchymal Stem Cells

Cited by 68 publications

References 55 publications

The use of mesenchymal stem cells for cartilage repair and regeneration: a systematic review

The use of mesenchymal stem cells for cartilage repair and regeneration: a systematic review

Silk microfiber-reinforced silk hydrogel composites for functional cartilage tissue repair

Effects of chondrogenic and osteogenic regulatory factors on composite constructs grown using human mesenchymal stem cells, silk scaffolds and bioreactors

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