Engineering of cartilage in recombinant human type II collagen gel in nude mouse model in vivo

Abstract

These results show that rhCII-gel provides good expansion and mechanical support for the formation of cartilage neotissue. RhCII material may allow favorable conditions in the repair of chondral lesions.

Five percent oxygen tension is not beneficial for neocartilage formation in scaffold-free cell cultures

“…Then, the fixed tissues were dehydrated with a series of gradually increasing concentrations of ethanol and embedded in tissue-Tek III embedding paraffin. Histological sections (5 μm in thickness) were cut from the middle part of the tissue and stained with toluidine blue (Serva, Heidelberg, Germany) as described previously (Pulkkinen et al 2010). Immunohistochemical staining was performed with anti-type II collagen mouse monoclonal antibody (Holmdahl et al 1986) and anti-type I collagen rabbit polyclonal antibody (Pulkkinen et al 2010).…”

“…The extracted PGs were separated in 1.2% agarose gels, as in our previous study (Pulkkinen et al 2010) after a small modification. Briefly, 5 μg extracted PGs was dissolved in 10 μl sample buffer (1% SDS in 1× TRIS/acetic acid/EDTA buffer) and boiled for 5 min in tightly capped, seal-containing plastic tubes.…”

Biomaterials for Cartilage Tissue Engineering

“…In another study, isolated bovine chondrocytes seeded into rhCII gels were injected subcutaneously into the back of nude mouse model. It was observed that this complex offered the ability to promote cell proliferation and mechanical strength for the formation of cartilage [25]. In a recent study, Wang et al used core-shell nanofibrous scaffold fabricated by collagen and poly(L-lactic acid-co-epsilon-caprolactone) to encapsulate rhTGF-β3 and bovine serum albumin into the core of the nanofibers for tracheal cartilage regeneration.…”

Control of swelling properties of polyvinyl alcohol/hyaluronic acid hydrogels for the encapsulation of chondrocyte cells

“…22 Cell isolation was performed similarly twice from one animal (once per knee). Viability of the cells was determined before the following investigations.…”