Engineering of bone using bone marrow stromal cells and a silicon-stabilized tricalcium phosphate bioceramic: Evidence for a coupling between bone formation and scaffold resorption

Mastrogiacomo, M.; Papadimitropoulos, Adam; Cedola, Alessia; Peyrin, Françoise; Giannoni, Paolo; Pearce, Simon G.; Alini, Mauro; Giannini, Cinzia; Guagliardi, Antonietta; Cancedda, Ranieri

doi:10.1016/j.biomaterials.2006.10.001

Cited by 132 publications

(83 citation statements)

References 30 publications

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“…The bone mineral density and bone strength in sheep is increased relative to human. Moreover, sheep models of critical size defects are extensively applied to evaluate cell-based therapeutic approaches using autologues, allogeneic or xenogeneic MPCs in combination with growth factors and different types of scaffolds to enhance bone regeneration showing significant advantages compared with cell-unloaded (empty) scaffolds [43,53,166,[169][170][171][172][173][174][175][176].…”

Section: Sheepmentioning

confidence: 99%

The rational use of animal models in the evaluation of novel bone regenerative therapies

Perić¹,

Dumić-Čule²,

Grčević³

et al. 2015

Bone

126

116

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Section: Sheepmentioning

confidence: 99%

The rational use of animal models in the evaluation of novel bone regenerative therapies

Perić¹,

Dumić-Čule²,

Grčević³

et al. 2015

Bone

126

116

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“…In this context the three-dimensional (3D) nondestructive X-ray computed microtomography (microCT) may contribute to a better understanding of the biodegradation processes and therefore, to a better fulfillment of the tissue engineering needs for the development of the future generation biomaterials (Komlev et al, 2006;. Several publications are dealing with studies by microCT of the biodegradation of the implanted porous scaffolds (Papadimitropoulos et al, 2007;Renghini et al, 2009). All these studies compared in different scaffolds the percentage of the total sample volume fracture or the thickness of the investigated biomaterials either before (control) or after implantation.…”

Section: Biodegradation Of Porous Calcium Phosphate Scaffolds In An Ementioning

confidence: 99%

“…In these investigations, undecalcified histological sections were stained for tartrate-resistant acid phosphatase activity to reveal the possible presence of cells with osteoclastic activity close to the calcium phosphate bone substitute materials. On the contrary, other authors reported that scaffold biodegradation occurred mainly as result of an osteoclastic activity (Schepers et al, 1991;Mastrogiacomo et al, 2007). The latter mechanism of biodegradation is to be preferred (Schenk, 1991;Rumpel et al, 2006), because mimicry of the physiological bone resorption process should create optimal surfaces for colonization by osteoblasts and vascular tissue.…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of porous calcium phosphate scaffolds in an ectopic bone formation model studied by X-ray computed microtomograph

Komlev

Mastrogiacomo²,

Pereira

et al. 2010

eCM

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Three types of ceramic scaffolds with different composition and structure [namely synthetic 100% hydroxyapatite (HA; Engipore), synthetic calcium phosphate multiphase biomaterial containing 67% silicon stabilized tricalcium phosphate (Si-TCP; Skelite™) and natural bone mineral derived scaffolds (Bio-oss®)] were seeded with mesenchymal stem cells (MSC) and ectopically implanted for 8 and 16 weeks in immunodeficient mice. X-ray synchrotron radiation microtomography was used to derive 3D structural information on the same scaffolds both before and after implantation. Meaningful images and morphometric parameters such as scaffold and bone volume fraction, mean thickness and thickness distribution of the different phases as a function of the implantation time, were obtained. The used imaging algorithms allowed a direct comparison and registration of the 3D structure before and after implantation of the same sub-volume of a given scaffold. In this way it was possible to directly monitor the tissue engineered bone growth and the complete or partial degradation of the scaffold. Further, the detailed kinetics studies on Skelite™ scaffolds implanted for different length of times from 3 days to 24 weeks, revealed in the X-ray absorption histograms two separate peaks associated to HA and TCP. It was therefore possible to observe that the progressive degradation of the Skelite™ scaffolds was mainly due to the resorption of TCP. The different saturation times in the tissue engineered bone growth and in the TCP resorption confirmed that the bone growth was not limited the scaffold regions that were resorbed but continued in the inward direction with respect to the pore surface.

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“…Although the latter presents a major potential for skeletal regeneration procedures, most in vivo studies are conducted using an ectopic approach and/or performed in small animal models, such as mice or rats (Kruyt et al, 2007;Livington et al, 2002;Mauney et al, 2005a;Mastrogiacomo et al, 2007;Mendes et al, 2003;Trojani et al, 2006). Although non-critical sized defects are usually evaluated in ectopic models, orthotopic location provides a more accurate idea of the influence or local effects of implanted cells or cell-scaffold constructs where they were initially designed to be functional.…”

Section: Introductionmentioning

confidence: 99%

Tissue-engineered constructs based on SPCL scaffolds cultured with goat marrow cells: functionality in femoral defects

Rodrigues

Gomes

Viegas

et al. 2010

J Tissue Eng Regen Med

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This study aims to assess the in vivo performance of cell-scaffold constructs composed of goat marrow stromal cells (GBMCs) and SPCL (a blend of starch with polycaprolactone) fibre mesh scaffolds at different stages of development, using an autologous model. GBMCs from iliac crests were seeded onto SPCL scaffolds and in vitro cultured for 1 and 7 days in osteogenic medium. After 1 and 7 days, the constructs were characterized for proliferation and initial osteoblastic expression by alkaline phosphatase (ALP) activity. Scanning electron microscopy analysis was performed to investigate cellular morphology and adhesion to SPCL scaffolds. Non-critical defects (diameter 6 mm, depth 3 mm) were drilled in the posterior femurs of four adult goats from which bone marrow and serum had been collected previously. Drill defects alone and defects filled with scaffolds without cells were used as controls. After implantation, intravital fluorescence markers, xylenol orange, calcein green and tetracycline, were injected subcutaneously after 2, 4 and 6 weeks, respectively, for bone formation and mineralization monitoring. Subsequently, samples were stained with Lévai-Laczkó for bone formation and histomorphometric analysis. GBMCs adhered and proliferated on SPCL scaffolds and an initial differentiation into pre-osteoblasts was detected by an increasing level of ALP activity with the culture time. In vivo experiments indicated that bone neoformation occurred in all femoral defects. The results obtained provided important information about the performance of SPCL-GBMC constructs in an orthotopic goat model that enabled future studies to be designed to investigate in vivo the functionality of SPCL-GBMC constructs in more complex models, viz. critical sized defects, and to evaluate the influence of in vitro cultured autologous cells in the healing and bone regenerative process.

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Engineering of bone using bone marrow stromal cells and a silicon-stabilized tricalcium phosphate bioceramic: Evidence for a coupling between bone formation and scaffold resorption

Cited by 132 publications

References 30 publications

The rational use of animal models in the evaluation of novel bone regenerative therapies

The rational use of animal models in the evaluation of novel bone regenerative therapies

Biodegradation of porous calcium phosphate scaffolds in an ectopic bone formation model studied by X-ray computed microtomograph

Tissue-engineered constructs based on SPCL scaffolds cultured with goat marrow cells: functionality in femoral defects

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