Engineering of a novel pluronic F127/graphene nanohybrid for pH responsive drug delivery

Abstract

Herein, a novel Pluronic F127/graphene nanosheet (PF127/GN) hybrid was prepared via an one-pot process including the simultaneous reduction of graphene oxide and assembly of PF127 and GN. The nanohybrid exhibits high water dispersibility and stability in physiological environment with the hydrophilic chains of PF127 extending to the solution while the hydrophobic segments anchoring at the surface of graphene via hydrophobic interaction. The PF127/GN nanohybrid is found to be capable of effectively encapsulating doxorubicin (DOX) with ultrahigh drug-loading efficiency (DLE; 289%, w/w) and exhibits a pH responsive drug release behavior. The superb DLE of the PF127/GN nanohybrid relies on the introduction of GN which is structurally compatible with DOX. Cellular toxicity assays performed on human breast cancer MCF-7 cells demonstrate that the PF127/GN nanohybrid displays no obvious cytotoxicity, whereas the PF127/GN-loaded DOX (PF127/GN/DOX) shows remarkable cytotoxicity to the MCF-7. Cell internalization study reveals that PF127/GN nanohybrid facilitates the transfer of DOX into MCF-7 cells, evidenced by the image of confocal laser scanning microscopy. The above results indicate the potential application of this novel nanocarrier in biomedicine.

Preparation of Fe3O4/Reduced Graphene Oxide Nanocomposites with Good Dispersibility for Delivery of Paclitaxel

“…After loading with PTX ( Figure 5(d)), Fe 3 O 4 nanoparticles were still firmly anchored on the surface of RGO. Figure 6(a), 40.4% mass loss before 200 ∘ C was due to desorption of H 2 O and pyrolysis of oxygen-containing group [24]. The pyrolysis of carbon skeleton was exhibited at 50.3% mass loss between 200 and 550 ∘ C. Figure 6(b) showed that 17.4% weight loss before 300 ∘ C was the removal of labile groups.…”

“…Secondly, PF127 (400 mg) was dropped to Fe 3 O 4 /f-GO solution (15 mL, 1.0 mg/mL) and hydrazine monohydrate (400 L) was then added to reduce the GO [24]. After reaction, Fe 3 O 4 /RGO nanocomposites were obtained by pressure filtration.…”

Synthesis, toxicity, biocompatibility, and biomedical applications of graphene and graphene-related materials

“…The toxic effects of graphene can be influenced by physicochemical properties such as size and distribution, surface charge, surface area, layer number, lateral dimensions, surface chemistry, purity, particulate state, surface functional groups, and shape. 31,146,147 The mechanisms by which GO and rGO nanowalls show toxicity to E. coli and Staphylococcus aureus bacteria were demonstrated by Akhavan and Ghaderi 28 and Hu et al 148 They demonstrated that both GO and rGO are effective as antibacterial agents. Subsequently, many research laboratories have investigated the potential toxicity of GO and rGO against several bacterial species.…”

Bioimpact of Carbon Nanomaterials

“…GO was widely investigated as a nanocarrier with numerous chemotherapy drugs: doxorubicin [203,204,154,205], camptothecin [206,207], ellagic acid [208], 1,3-bis(2-chloroethyl)-1-nitrosourea [209], and b-lapachone [210]. Sun et al [193] have synthesized a conjugate of PEGylated GO with an anti-CD20 antibody, rutixan and doxorubicin.…”