Engineering Nanoporous Bioactive Smart Coatings Containing Microorganisms: Fundamentals and Emerging Applications

Flickinger, Michael C.; Fidaleo, Marcello; Gosse, Laurent; Polzin, Kayla M.; Charaniya, Salim; Solheid, C.; Lyngberg, Olav; Laudon, M.; Ge, Hong You; Schottel, Janet L.; Bond, Daniel R.; Aksan, Alptekin; Scriven, L. E.

doi:10.1021/bk-2009-1002.ch003

Cited by 6 publications

(18 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In [81], nonporous adhesive latex coatings and inkjet deposited latex microstructures, containing concentrated viable but non-growing microorganisms were presented. Such nontoxic (low biocide or biocide-free) latex emulsions with carbohydrate porogens which were used for generating nanopores can be used for smart coatings.…”

Section: Smart Emulsionsmentioning

confidence: 99%

Smart and green interfaces: From single bubbles/drops to industrial environmental and biomedical applications

Dutschk

Karapantsios

Liggieri

et al. 2014

Advances in Colloid and Interface Science

View full text Add to dashboard Cite

Interfaces can be called Smart and Green (S&G) when tailored such that the required technologies can be implemented with high efficiency, adaptability and selectivity. At the same time they have also to be eco-friendly, i.e. products must be biodegradable, reusable or simply more durable. Bubble and drop interfaces are in many of these smart technologies the fundamental entities and help develop smart products of the everyday life.Significant improvements of these processes and products can be achieved by implementing and manipulating specific properties of these interfaces in a simple and smart way, in order to accomplish specific tasks. The severe environmental issues require in addition attributing ecofriendly features to these interfaces, by incorporating innovative , or, sometime, recycle materials and conceiving new production processes which minimize the use of natural resources and energy. Such concept can be extended to include important societal challenges related to support a sustainable development and a healthy population.The achievement of such ambitious targets requires the technology research to be supported by a robust development of theoretical and experimental tools, needed to understand in more details the behavior of complex interfaces. A wide but not exhaustive review of recent work concerned with green and smart interfaces is presented, addressing different scientific and technological fields. The presented approaches reveal a huge potential in relation to various technological fields, such as nanotechnologies, biotechnologies, medical diagnostics, new or improved materials.

show abstract

Section: Smart Emulsionsmentioning

confidence: 99%

Smart and green interfaces: From single bubbles/drops to industrial environmental and biomedical applications

Dutschk

Karapantsios

Liggieri

et al. 2014

Advances in Colloid and Interface Science

View full text Add to dashboard Cite

show abstract

“…Cantwell first reported the use of polymer blends of hard and soft polymer particles for microbial entrapment, but did not fabricate thin coatings—only flocculates, 1–2 mm aggregates, and 2 mm diameter fibrils [21,48]. Martens and Hall reported methylmethacrylate and butyl acrylate polymer coatings of photosynthetic Synechococcus on a carbon electrode, creating a functional biomimetic device that showed cell viability of “nearly 100%” with photoreactivity after rehydration and exposure to light [21,49].…”

Section: Early Cellular and Particle Array Composite Coating Systemsmentioning

confidence: 99%

Engineering Cellular Photocomposite Materials Using Convective Assembly

2013

View full text Add to dashboard Cite

Fabricating industrial-scale photoreactive composite materials containing living cells, requires a deposition strategy that unifies colloid science and cell biology. Convective assembly can rapidly deposit suspended particles, including whole cells and waterborne latex polymer particles into thin (<10 µm thick), organized films with engineered adhesion, composition, thickness, and particle packing. These highly ordered composites can stabilize the diverse functions of photosynthetic cells for use as biophotoabsorbers, as artificial leaves for hydrogen or oxygen evolution, carbon dioxide assimilation, and add self-cleaning capabilities for releasing or digesting surface contaminants. This paper reviews the non-biological convective assembly literature, with an emphasis on how the method can be modified to deposit living cells starting from a batch process to its current state as a continuous process capable of fabricating larger multi-layer biocomposite coatings from diverse particle suspensions. Further development of this method will help solve the challenges of engineering multi-layered cellular photocomposite materials with high reactivity, stability, and robustness by clarifying how process, substrate, and particle parameters affect coating microstructure. We also describe how these methods can be used to selectively immobilize photosynthetic cells to create biomimetic leaves and compare these biocomposite coatings to other cellular encapsulation systems.

show abstract

“…This requires engineering nontoxic adhesive polymers capable of permanently entrapping and stabilizing a high density of any viable cell to any surface in a thin (<100 μm thick) nanoporous coating with minimal diffusion resistance. These thin composite polymer coatings, known as biocatalytic coatings and microbial inks, could revolutionize the use of microbes as biocatalysts (1, 2).…”

Section: 0 Introductionmentioning

confidence: 99%

“…The development of waterborne latex coating technologies over the past 2 decades with lower volatile organic content (VOC) in order to comply with environmental regulations has produced emulsions with low solvent toxicity (3). The polymer chemistry and film‐forming properties of these waterborne emulsions can be modified to generate adhesive nanoporous latex coatings for permanent entrapment of living cells (1).…”

Section: 0 Introductionmentioning

confidence: 99%

Painting and Printing Living Bacteria: Engineering Nanoporous Biocatalytic Coatings to Preserve Microbial Viability and Intensify Reactivity

Flickinger

Schottel

Bond

et al. 2007

Biotechnology Progress

Self Cite

100

View full text Add to dashboard Cite

Latex biocatalytic coatings containing ∼50% by volume of microorganisms stabilize, concentrate and preserve cell viability on surfaces at ambient temperature. Coatings can be formed on a variety of surfaces, delaminated to generate stand-alone membranes or formulated as reactive inks for piezoelectric deposition of viable microbes. As the latex emulsion dries, cell preservation by partial desiccation occurs simultaneously with the formation of pores and adhesion to the substrate. The result is living cells permanently entrapped, surrounded by nanopores generated by partially coalesced polymer particles. Nanoporosity is essential for preserving microbial viability and coating reactivity. Cryo-SEM methods have been developed to visualize hydrated coating microstructure, confocal microscopy and dispersible coating methods have been developed to quantify the activity of the entrapped cells, and FTIR methods are being developed to determine the structure of vitrified biomolecules within and surrounding the cells in dry coatings. Coating microstructure, stability and reactivity are investigated using small patch or strip coatings where bacteria are concentrated 10 2 -to 10 3 -fold in 5-75 µm thick layers with pores formed by carbohydrate porogens. The carbohydrate porogens also function as osmoprotectants and are postulated to preserve microbial viability by formation of glasses inside the microbes during coat drying; however, the molecular mechanism of cell preservation by latex coatings is not known. Emerging applications include coatings for multistep oxidations, photoreactive coatings, stabilization of hyperthermophiles, environmental biosensors, microbial fuel cells, as reaction zones in microfluidic devices, or as very high intensity (>100 g‚L -1 coating volume‚h -1 ) industrial or environmental biocatalysts. We anticipate expanded use of nanoporous adhesive coatings for prokaryotic and eukaryotic cell preservation at ambient temperature and the design of highly reactive "living" paints and inks.

show abstract

Engineering Nanoporous Bioactive Smart Coatings Containing Microorganisms: Fundamentals and Emerging Applications

Cited by 6 publications

References 0 publications

Smart and green interfaces: From single bubbles/drops to industrial environmental and biomedical applications

Smart and green interfaces: From single bubbles/drops to industrial environmental and biomedical applications

Engineering Cellular Photocomposite Materials Using Convective Assembly

Painting and Printing Living Bacteria: Engineering Nanoporous Biocatalytic Coatings to Preserve Microbial Viability and Intensify Reactivity

Contact Info

Product

Resources

About