Engineering Nanoparticles for Targeted Delivery of Nucleic Acid Therapeutics in Tumor

Xiao, Yao; Shi, Kun; Qu, Ying; Chu, Bingyang; Zhang, Qian

doi:10.1016/j.omtm.2018.09.002

Cited by 109 publications

(68 citation statements)

References 202 publications

(222 reference statements)

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“…Al-Attar and collaborators evaluated a combination of drug delivery devices composed of holo-transferrin conjugated liposomes for siRNA (targeting BCR-ABL) delivery, and electrospun polycaprolactone-gelatin microfibers for resveratrol release [215]. For additional applications on liposomes for gene therapy see [50,209,[216][217][218][219].…”

Section: Lipid Basedmentioning

confidence: 99%

Gene Therapy in Cancer Treatment: Why Go Nano?

et al. 2020

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The proposal of gene therapy to tackle cancer development has been instrumental for the development of novel approaches and strategies to fight this disease, but the efficacy of the proposed strategies has still fallen short of delivering the full potential of gene therapy in the clinic. Despite the plethora of gene modulation approaches, e.g., gene silencing, antisense therapy, RNA interference, gene and genome editing, finding a way to efficiently deliver these effectors to the desired cell and tissue has been a challenge. Nanomedicine has put forward several innovative platforms to overcome this obstacle. Most of these platforms rely on the application of nanoscale structures, with particular focus on nanoparticles. Herein, we review the current trends on the use of nanoparticles designed for cancer gene therapy, including inorganic, organic, or biological (e.g., exosomes) variants, in clinical development and their progress towards clinical applications.

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Section: Lipid Basedmentioning

confidence: 99%

Gene Therapy in Cancer Treatment: Why Go Nano?

et al. 2020

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“…For DNA vaccines as well as general nucleic acid delivery applications, lipid-based NPs are another prominent class of material used as non-viral vectors [67]. Liposomes have been a widely studied lipid-based delivery system for nucleic acid delivery due to their high transfection and encapsulation efficiency, as well as their easily tunable surface properties [68]. However, there are several challenges that hinder the transition of liposomes to clinical studies which include toxicity, nonspecific immunogenicity, instability in circulation, and rapid clearance from the body.…”

Section: Lipid Nanoparticlesmentioning

confidence: 99%

Engineered Nanodelivery Systems to Improve DNA Vaccine Technologies

et al. 2020

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DNA vaccines offer a flexible and versatile platform to treat innumerable diseases due to the ease of manipulating vaccine targets simply by altering the gene sequences encoded in the plasmid DNA delivered. The DNA vaccines elicit potent humoral and cell-mediated responses and provide a promising method for treating rapidly mutating and evasive diseases such as cancer and human immunodeficiency viruses. Although this vaccine technology has been available for decades, there is no DNA vaccine that has been used in bed-side application to date. The main challenge that hinders the progress of DNA vaccines and limits their clinical application is the delivery hurdles to targeted immune cells, which obstructs the stimulation of robust antigen-specific immune responses in humans. In this updated review, we discuss various nanodelivery systems that improve DNA vaccine technologies to enhance the immunological response against target diseases. We also provide possible perspectives on how we can bring this exciting vaccine technology to bedside applications.

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“…Therapeutic applications of nanoparticles (NPs) are very diverse. One of them is the treatment of cancer (Li and Pu 2019;Mottaghitalab et al 2019;Xiao et al 2019). Some chemotherapeutic drugs have already been successfully attached to the surface of nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

Modification of fluorescent nanocrystals with 6-thioguanine: monitoring of drug delivery

et al. 2019

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The purpose of the presented work was to modify the surface of core/shell-type CdSe x S 1-x /ZnS quantum dots (QDs) with an anticancer drug to form bifunctional nanoconjugate for imaging and drug delivery. 6-Thioguanine (6-TG) which is used to treat acute myeloid and lymphoblastic leukemia was applied for this aim. The modification of the nanocrystals was carried out using a biphasic method, based on transferring of QDs from the organic phase to the aqueous one with the simultaneous exchange of hydrophobic ligand to the hydrophilic one. To enable a complex evaluation of 6-TG bioactivity, the surface of quantum dots was also modified using mercaptoacetic acid (MAA). Mercaptoacetic acid was chosen as a biologically neutral ligand; so, it was possible to evaluate the effect of nanocrystals toxicity without the participation of the drug (6-TG ligand versus neutral ligand). Moreover, the effect of 6-TG itself on cells was also studied. The studies were carried out on three cell lines: K562 (human myeloid leukemia), A549 (human lung cancer cells) and MRC-5 (normal human lung cells). Evaluation of the cytotoxicity of nanostructures, at various concentrations, was carried out after 24-and 48-h incubation of the cell culture with nanoparticles/drug solutions. MTT and the Alamar Blue assays were used to determine cell viability. Based on the cytotoxicity measurements, it was found that quantum dots modified with 6-TG are more toxic than the drug itself or QDs modified with 3-mercaptoacetic acid. Images taken using fluorescence and confocal microscopy allowed to define the location of examined QDs inside cells.

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Engineering Nanoparticles for Targeted Delivery of Nucleic Acid Therapeutics in Tumor

Cited by 109 publications

References 202 publications

Gene Therapy in Cancer Treatment: Why Go Nano?

Gene Therapy in Cancer Treatment: Why Go Nano?

Engineered Nanodelivery Systems to Improve DNA Vaccine Technologies

Modification of fluorescent nanocrystals with 6-thioguanine: monitoring of drug delivery

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